Detailed information NTG1021560

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V5214_RS04495
• Genome accession: NZ_CP160495
• Coordinates: 950201..951001 (-)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 50Gaza2023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 945201..956001

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5214_RS04475	dksA	945780..946235 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
V5214_RS04480	sfsA	946398..947114 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
V5214_RS04485	thpR	947114..947651 (-)	538	Protein_856	RNA 2',3'-cyclic phosphodiesterase	-
V5214_RS04490	hrpB	947724..950153 (+)	2430	WP_064472269.1	ATP-dependent helicase HrpB	-
V5214_RS04495	pilD	950201..951001 (-)	801	WP_004191960.1	prepilin peptidase	Machinery gene
V5214_RS04500	-	950998..951744 (-)	747	WP_004145897.1	type II secretion system protein N	-
V5214_RS04505	-	951734..952219 (-)	486	WP_004145896.1	type II secretion system protein M	-
V5214_RS04510	gspL	952212..953405 (-)	1194	WP_032416685.1	type II secretion system protein GspL	-
V5214_RS04515	gspK	953392..954378 (-)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
V5214_RS04520	gspJ	954375..954971 (-)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
V5214_RS04525	gspI	954968..955333 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
V5214_RS04530	gspH	955330..955845 (-)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=1021560 V5214_RS04495 WP_004191960.1 950201..951001(-) (pilD) [Klebsiella pneumoniae strain 50Gaza2023]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=1021560 V5214_RS04495 WP_004191960.1 950201..951001(-) (pilD) [Klebsiella pneumoniae strain 50Gaza2023]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421