Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QRP18_RS14285 Genome accession   NZ_CP160382
Coordinates   3082156..3083172 (-) Length   338 a.a.
NCBI ID   WP_140062950.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain ST110-8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3077156..3088172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRP18_RS14260 (QRP18_14260) aroK 3078016..3078534 (-) 519 WP_005381319.1 shikimate kinase AroK -
  QRP18_RS14265 (QRP18_14265) pilQ 3078723..3080471 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  QRP18_RS14270 (QRP18_14270) pilP 3080503..3081018 (-) 516 WP_025554074.1 pilus assembly protein PilP Machinery gene
  QRP18_RS14275 (QRP18_14275) pilO 3081008..3081601 (-) 594 WP_110178237.1 type 4a pilus biogenesis protein PilO Machinery gene
  QRP18_RS14280 (QRP18_14280) pilN 3081594..3082172 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  QRP18_RS14285 (QRP18_14285) pilM 3082156..3083172 (-) 1017 WP_140062950.1 type IV pilus biogenesis protein PilM Machinery gene
  QRP18_RS14290 (QRP18_14290) - 3083315..3085885 (+) 2571 WP_140062951.1 penicillin-binding protein 1A -
  QRP18_RS14295 (QRP18_14295) oxyR 3085941..3086849 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  QRP18_RS14300 (QRP18_14300) - 3087020..3087748 (+) 729 WP_140062952.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37771.29 Da        Isoelectric Point: 6.8850

>NTDB_id=1020564 QRP18_RS14285 WP_140062950.1 3082156..3083172(-) (pilM) [Vibrio parahaemolyticus strain ST110-8]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RHVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1020564 QRP18_RS14285 WP_140062950.1 3082156..3083172(-) (pilM) [Vibrio parahaemolyticus strain ST110-8]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCAAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCACGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment