Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AB0R75_RS05130 Genome accession   NZ_CP160232
Coordinates   1058160..1061864 (+) Length   1234 a.a.
NCBI ID   WP_335348914.1    Uniprot ID   -
Organism   Bacillus pumilus strain JJ1622     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1053160..1066864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R75_RS05115 (AB0R75_05115) - 1053454..1053864 (+) 411 WP_335348916.1 DoxX family protein -
  AB0R75_RS05120 (AB0R75_05120) - 1054062..1054508 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  AB0R75_RS05125 (AB0R75_05125) addB 1054673..1058182 (+) 3510 WP_335348915.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB0R75_RS05130 (AB0R75_05130) addA 1058160..1061864 (+) 3705 WP_335348914.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB0R75_RS05135 (AB0R75_05135) sbcD 1061891..1063066 (+) 1176 WP_144522815.1 exonuclease subunit SbcD -
  AB0R75_RS05140 (AB0R75_05140) sbcC 1063063..1066458 (+) 3396 WP_335348913.1 exonuclease subunit SbcC -
  AB0R75_RS05145 (AB0R75_05145) - 1066478..1066777 (+) 300 WP_144522817.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142237.34 Da        Isoelectric Point: 5.2144

>NTDB_id=1020249 AB0R75_RS05130 WP_335348914.1 1058160..1061864(+) (addA) [Bacillus pumilus strain JJ1622]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPKQWMRAFLSLYDVNTQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPIVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSSEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEKGEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLKQRDLLTDEQVQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYSGGIDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNVEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1020249 AB0R75_RS05130 WP_335348914.1 1058160..1061864(+) (addA) [Bacillus pumilus strain JJ1622]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
AGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACACC
CAGTTGATGTGGATCGACTGCTCGTTGTGACTTTCACAAACGCATCTGCAGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTATCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTACGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGGAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACATCTGACCGTCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCAAGATCTCATCCTTCTCCGAAGCAATGGATGAGGGCGTTCCTTTCACTTTATGATGTAAATACTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCCTATATCAAAGAAGATCTTTCGCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAGAAAGCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCGATTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGCTTCGAAAGAGCGAAGCAGGAAAAGTCTATTGTCGACTTTTCCGATTTAGAGCACT
ATTGCTTGCGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACAATTTTAAAACTTGTATCTAAAGGAGA
ACATTCTTCAGAGGGTAATCTGTTTATGGTTGGTGATGTCAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGT
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGGGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAGCAGGAAGCAGAAACGGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCCTTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGGAACTTGCAGTATCGAGACAT
TGTCATTTTACTTCGCTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATCCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAGTTGGCACTGATTCGGACGAGTGA
TAAAAAAGGTACGTATTATGATGCTGTAAAGGCCTTTATGAGTGTGACTCATTCTGATCATCCTACATGCAAAAAACTAG
AACGATTTTTCCAATTACTGCGCAAATGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGCGATACACAGTATCTTGATTACGTTGGGGGAATGCCTGGAGGTAAGCAGCGGCAAGCGAATCTTCGCGCCCTTTATGA
CCGAGCCAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTACGTTTTATTGAAAGAATGCAGGAACGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATTCATAGTAGTAAAGGA
TTAGAATTCCCAGTCGTCTTTACCGCAGGACTTGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAGCTAAGGGTTTTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCAGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGAAAGGCGAGATTGTACAAGCATTGATGGATGGTCTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCCATGCATACGGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAGTCACGTATAGGACACTTGCTCGAATCCTTAAAGCAGC
GAGACTTACTAACAGATGAGCAGGTTCAGTCAATTAATCAAGAGGGAATCGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACGGAGGGAGAGCCTGTTCTAATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTATTAG
ATTATAAAACTGATAGAGTTCAAGGCAGATATTCAGGTGGAATTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAA
ACCCAAATCGCGTTATATGCAAAAGCGGTAGAGCGTTTGACAAACAGAACTTTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATGTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.343

100

0.665

Multiple sequence alignment