Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AB0R75_RS05125 Genome accession   NZ_CP160232
Coordinates   1054673..1058182 (+) Length   1169 a.a.
NCBI ID   WP_335348915.1    Uniprot ID   -
Organism   Bacillus pumilus strain JJ1622     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1049673..1063182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R75_RS05095 (AB0R75_05095) lepB 1050183..1050695 (+) 513 WP_041815362.1 signal peptidase I -
  AB0R75_RS05100 (AB0R75_05100) - 1050731..1051876 (-) 1146 WP_144522837.1 AbrB family transcriptional regulator -
  AB0R75_RS05105 (AB0R75_05105) - 1052143..1052565 (+) 423 WP_003210872.1 BlaI/MecI/CopY family transcriptional regulator -
  AB0R75_RS05110 (AB0R75_05110) - 1052558..1053397 (+) 840 WP_144522811.1 M56 family metallopeptidase -
  AB0R75_RS05115 (AB0R75_05115) - 1053454..1053864 (+) 411 WP_335348916.1 DoxX family protein -
  AB0R75_RS05120 (AB0R75_05120) - 1054062..1054508 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  AB0R75_RS05125 (AB0R75_05125) addB 1054673..1058182 (+) 3510 WP_335348915.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB0R75_RS05130 (AB0R75_05130) addA 1058160..1061864 (+) 3705 WP_335348914.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB0R75_RS05135 (AB0R75_05135) sbcD 1061891..1063066 (+) 1176 WP_144522815.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134720.66 Da        Isoelectric Point: 5.1636

>NTDB_id=1020248 AB0R75_RS05125 WP_335348915.1 1054673..1058182(+) (addB) [Bacillus pumilus strain JJ1622]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISVESMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLAQQIPAAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKVTAAFTVDQS
YHDRQPSELDLFRMTGKTYFQLYQLAKECGADISENILERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSASK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKKLKKAQKVREMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSPLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLEPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCITYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGIEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQIIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1020248 AB0R75_RS05125 WP_335348915.1 1054673..1058182(+) (addB) [Bacillus pumilus strain JJ1622]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGACTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTAGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCTGAGGATATTGAGAAAATTTCAGTGGAGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAAATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTACTTGCCCAGCAAATTCCTGCAGCTGAAGAAATAAAAGGAGCACATATTTATATCGATGGTTTTTATCAATTTA
CACCGCAACAGCTTCTTGTCATCGAACAGCTCTTGCTGCATGCAGCAAAAGTAACTGCGGCTTTTACAGTAGATCAATCC
TATCATGATAGGCAGCCAAGTGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCGAA
AGAGTGCGGAGCTGACATTTCTGAAAATATTCTTGAAAGAAATCATCGACACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTGCAACCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAAGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTTAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATCGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTAAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCACGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTACGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTAAAAGAATGG
ATCGCGCCTCCGCTTTACCAGTTGCAGAAAAAGCTTAAAAAAGCACAAAAGGTAAGGGAGATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGACTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTCATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTAATTCCGCCATCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCTTCTGATGAAAGTGTATTATCTGAAGATGACCGAGAATGGTTAAAGCGAGCAGGAGCAGAGTTGGCA
GAGACAGGAAAGGAACGACTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTATATTT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGCCACTTATCAAGTATTGCCAGGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCTTATACCGTCTCACAGCTCCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAATATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CATTAGAGCCTAATGTCACAAAAGAGCTTTACGGTGATCATATTCAAGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGGCAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTATCACCTATTCGAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGTTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTAATTCGAGTTTCTTCTATATTAAGTGAACATGCCAAGGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGACCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTAGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATTGTC
GATTACAAATCTAGTGAGAAAGGTCTTGACCTAGCAGAGGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTCACCATTACGTATTCGAAGGAGTGGCTAGGCATAGAAGCAACGCCTGCTGGTATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGCTTA
TTGCTTGAAGATGTGGAAGCAGTCAAGCTAATGGATCAAACGCTTGATTCAGGTCGATCACAAATCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCTGCGGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGTGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGTCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.408

99.316

0.66

Multiple sequence alignment