Detailed information NTG1019996

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R65_RS12295
• Genome accession: NZ_CP160225
• Coordinates: 2544527..2545141 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ1284
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2539527..2550141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R65_RS12265 (AB0R65_12265)	rpsT	2539830..2540096 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R65_RS12270 (AB0R65_12270)	holA	2540113..2541154 (-)	1042	Protein_2369	DNA polymerase III subunit delta	-
AB0R65_RS12275 (AB0R65_12275)	-	2541194..2541345 (-)	152	Protein_2370	hypothetical protein	-
AB0R65_RS12280 (AB0R65_12280)	-	2541386..2541520 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R65_RS12285 (AB0R65_12285)	comEC	2541539..2543890 (-)	2352	WP_167340757.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R65_RS12290 (AB0R65_12290)	-	2543891..2544460 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R65_RS12295 (AB0R65_12295)	comEA	2544527..2545141 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R65_RS12300 (AB0R65_12300)	comER	2545200..2546021 (+)	822	WP_015240246.1	late competence protein ComER	-
AB0R65_RS12305 (AB0R65_12305)	-	2546090..2546827 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
AB0R65_RS12310 (AB0R65_12310)	rsfS	2546824..2547180 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R65_RS12315 (AB0R65_12315)	yqeK	2547198..2547758 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R65_RS12320 (AB0R65_12320)	-	2547748..2548317 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R65_RS12325 (AB0R65_12325)	yhbY	2548328..2548618 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
AB0R65_RS12330 (AB0R65_12330)	aroE	2548612..2549454 (-)	843	WP_015240248.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=1019996 AB0R65_RS12295 WP_015240245.1 2544527..2545141(-) (comEA) [Bacillus velezensis strain JJ1284]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019996 AB0R65_RS12295 WP_015240245.1 2544527..2545141(-) (comEA) [Bacillus velezensis strain JJ1284]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363