Detailed information NTG1019402

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R74_RS12250
• Genome accession: NZ_CP160216
• Coordinates: 2527864..2528478 (-)
• Length: 204 a.a.
• NCBI ID: WP_020956097.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ1043
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2522864..2533478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R74_RS12220 (AB0R74_12220)	rpsT	2523167..2523433 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R74_RS12225 (AB0R74_12225)	holA	2523450..2524491 (-)	1042	Protein_2360	DNA polymerase III subunit delta	-
AB0R74_RS12230 (AB0R74_12230)	-	2524531..2524682 (-)	152	Protein_2361	hypothetical protein	-
AB0R74_RS12235 (AB0R74_12235)	-	2524723..2524857 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R74_RS12240 (AB0R74_12240)	comEC	2524876..2527227 (-)	2352	WP_119026862.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R74_RS12245 (AB0R74_12245)	-	2527228..2527797 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R74_RS12250 (AB0R74_12250)	comEA	2527864..2528478 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R74_RS12255 (AB0R74_12255)	comER	2528537..2529358 (+)	822	WP_061046607.1	late competence protein ComER	-
AB0R74_RS12260 (AB0R74_12260)	-	2529427..2530164 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
AB0R74_RS12265 (AB0R74_12265)	rsfS	2530161..2530517 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R74_RS12270 (AB0R74_12270)	yqeK	2530535..2531095 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R74_RS12275 (AB0R74_12275)	-	2531085..2531654 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AB0R74_RS12280 (AB0R74_12280)	yhbY	2531665..2531955 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AB0R74_RS12285 (AB0R74_12285)	aroE	2531949..2532791 (-)	843	WP_366560961.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22251.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=1019402 AB0R74_RS12250 WP_020956097.1 2527864..2528478(-) (comEA) [Bacillus velezensis strain JJ1043]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019402 AB0R74_RS12250 WP_020956097.1 2527864..2528478(-) (comEA) [Bacillus velezensis strain JJ1043]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363