Detailed information NTG1015434

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: DQM96_RS23375
• Genome accession: NZ_LS483296
• Coordinates: 4470924..4473122 (+)
• Length: 732 a.a.
• NCBI ID: WP_073544816.1
• Uniprot ID: -
• Organism: Escherichia coli strain NCTC9966
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4465924..4478122

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM96_RS23355 (NCTC9966_04459)	metB	4467902..4469062 (-)	1161	WP_001295694.1	cystathionine gamma-synthase	-
DQM96_RS23360 (NCTC9966_04460)	metJ	4469339..4469656 (+)	318	WP_000852812.1	met regulon transcriptional regulator MetJ	-
DQM96_RS23365 (NCTC9966_04461)	yiiX	4469840..4470448 (+)	609	WP_000797353.1	YiiX family permuted papain-like enzyme	-
DQM96_RS23370 (NCTC9966_04462)	rpmE	4470509..4470721 (-)	213	WP_000710769.1	50S ribosomal protein L31	-
DQM96_RS23375 (NCTC9966_04463)	priA	4470924..4473122 (+)	2199	WP_073544816.1	primosomal protein N'	Machinery gene
DQM96_RS23380 (NCTC9966_04464)	cytR	4473278..4474303 (+)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
DQM96_RS23385 (NCTC9966_04465)	ftsN	4474395..4475354 (+)	960	WP_000068828.1	cell division protein FtsN	-
DQM96_RS23390 (NCTC9966_04466)	hslV	4475447..4475977 (+)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
DQM96_RS23395 (NCTC9966_04467)	hslU	4475987..4477318 (+)	1332	WP_001293341.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81769.06 Da
• Isoelectric Point: 8.9081

>NTDB_id=1015434 DQM96_RS23375 WP_073544816.1 4470924..4473122(+) (priA) [Escherichia coli strain NCTC9966]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLELINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=1015434 DQM96_RS23375 WP_073544816.1 4470924..4473122(+) (priA) [Escherichia coli strain NCTC9966]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTGGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCCCCCAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTACGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATACCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCAGGGAATGCGCGTCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACTCGTATGCGCCAGCATTTACAGGCTGATAACCAGGTCATTCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCACTGCTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAGCACCATCTGCGCTGCCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTCCCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTGTTCCCCGGCGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCAGAAGTACATCGCGGCGGCGCGC
GGATTTTGATTGGTACACAAATGCTGGCGAAAGGTCACCATTTCCCGGATGTGACGCTGGTTGCATTACTGGACGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTTGCTCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGGCGAATGATGCAGCTACCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCACTGGCAGA
CGAGAAACTGTGGGTTCTCGGTCCGGTTCCGGCTCTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAACGGTACGCTGGAGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• DEAD/DEAH box helicase (DEAD) (203-362)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	44.505	100	0.448