Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABV300_RS00615 Genome accession   NZ_CP159307
Coordinates   120789..122192 (+) Length   467 a.a.
NCBI ID   WP_353714647.1    Uniprot ID   -
Organism   Dehalogenimonas sp. 4OHTPN     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 115789..127192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV300_RS00580 (ABV300_00580) ispF 116355..116825 (-) 471 WP_353715395.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ABV300_RS00585 (ABV300_00585) ispD 116833..117525 (-) 693 WP_353714641.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ABV300_RS00590 (ABV300_00590) ltaE 117659..118684 (+) 1026 WP_353714642.1 low-specificity L-threonine aldolase -
  ABV300_RS00595 (ABV300_00595) - 118681..119301 (+) 621 WP_353714643.1 hypothetical protein -
  ABV300_RS00600 (ABV300_00600) - 119298..119546 (-) 249 WP_353714644.1 zinc ribbon domain-containing protein -
  ABV300_RS00605 (ABV300_00605) - 119562..119963 (-) 402 WP_353714645.1 NifB/NifX family molybdenum-iron cluster-binding protein -
  ABV300_RS00610 (ABV300_00610) - 120093..120605 (-) 513 WP_353714646.1 protease inhibitor I42 family protein -
  ABV300_RS00615 (ABV300_00615) radA 120789..122192 (+) 1404 WP_353714647.1 DNA repair protein RadA Machinery gene
  ABV300_RS00620 (ABV300_00620) - 122195..122542 (+) 348 WP_353714648.1 hypothetical protein -
  ABV300_RS00625 (ABV300_00625) - 122552..122734 (+) 183 WP_353714649.1 hypothetical protein -
  ABV300_RS00630 (ABV300_00630) - 122879..125407 (+) 2529 WP_353714650.1 ATP-dependent Clp protease ATP-binding subunit -
  ABV300_RS00635 (ABV300_00635) - 125666..127081 (+) 1416 WP_353714651.1 reductive dehalogenase -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 50072.62 Da        Isoelectric Point: 7.2261

>NTDB_id=1013125 ABV300_RS00615 WP_353714647.1 120789..122192(+) (radA) [Dehalogenimonas sp. 4OHTPN]
MPKSRVVFVCSGCGQESPKWQGKCPGCGEWNTLFEQTVAVVKATSLRRALTNAPQALSEVASTGHERIPLSMGEVSRVLG
GGLVPGSLILFGGEPGIGKSTLLLQVAAQTAESSKSPVLYVSGEETRPQIKMRAKRLGIEGGGLYLLNETDLDQIIAEMD
KLSPALVIIDSIQTVYTSDIEMAPGGVSQVRECAARLVTWAKASQTPVLIAGHVTKDGAIAGPRLLEHIVDTVLYLEGEP
FSSYRILRSVKNRYGSVNEVGIFEMKEDGLRQVDNPSEVFLSRDRQNTVGSAVVPVLEGSRPLLVEIQALTNMTSFGQPR
RTANGLDFGRLLIITAVLSRRAYLKLGTQDVIASVTGGLHVAEPAADLAAALAIASSYKNAPVDPQLVAIGEVGLSGEIR
NVPQIERRLAEADRLGFRKALVPVNARIKPPSTDFKLIPVRDIRQALREGLTAAREEPEAETGQCLL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=1013125 ABV300_RS00615 WP_353714647.1 120789..122192(+) (radA) [Dehalogenimonas sp. 4OHTPN]
ATGCCTAAGTCCCGCGTCGTCTTCGTCTGCTCCGGCTGCGGCCAGGAGTCCCCAAAATGGCAGGGCAAGTGTCCCGGCTG
CGGCGAGTGGAATACGCTCTTCGAGCAGACCGTCGCCGTCGTGAAGGCGACATCCCTCCGCCGCGCCCTCACTAACGCGC
CGCAGGCTCTGTCCGAAGTCGCTTCGACCGGCCACGAGCGTATCCCCCTTTCAATGGGCGAGGTCAGCCGGGTACTGGGC
GGCGGCCTGGTACCGGGATCTTTGATCCTTTTCGGCGGCGAGCCGGGCATCGGCAAGTCAACGCTGCTCCTTCAGGTGGC
GGCGCAGACCGCCGAATCGTCCAAATCGCCCGTGCTGTACGTTTCCGGCGAGGAAACGCGGCCGCAGATCAAGATGAGGG
CCAAGAGATTGGGCATCGAGGGCGGCGGGCTTTACCTCCTCAACGAGACCGACCTCGACCAGATCATCGCCGAGATGGAC
AAGCTCTCACCGGCCCTGGTCATCATCGATTCCATCCAGACGGTCTACACTTCTGACATCGAGATGGCCCCCGGCGGCGT
GTCCCAGGTGCGGGAGTGCGCCGCGCGCCTGGTGACCTGGGCCAAGGCGTCTCAGACGCCGGTACTAATCGCCGGGCACG
TCACCAAGGACGGCGCCATCGCCGGGCCGCGACTCCTGGAACACATCGTGGACACCGTTCTCTACCTGGAGGGCGAACCG
TTCTCCAGCTATCGCATCCTGCGGTCGGTCAAGAATCGCTACGGCTCGGTCAACGAGGTCGGCATCTTCGAGATGAAAGA
AGACGGCCTGCGGCAGGTGGACAACCCCTCCGAGGTCTTCTTATCGCGCGACCGCCAGAACACTGTCGGTTCGGCCGTGG
TGCCGGTGCTGGAGGGCTCCCGCCCTCTCCTCGTCGAAATCCAGGCCCTGACCAACATGACCAGCTTCGGCCAGCCGCGG
CGGACGGCCAACGGCCTCGACTTCGGGCGTCTGCTTATCATCACCGCCGTGCTGTCGCGGCGGGCTTACTTGAAACTGGG
GACGCAGGATGTCATCGCCTCGGTCACCGGCGGCCTCCACGTGGCGGAACCTGCCGCCGACCTGGCCGCCGCCCTGGCAA
TAGCATCGAGCTACAAAAACGCGCCGGTCGATCCGCAGCTTGTCGCCATCGGCGAAGTGGGTCTCTCCGGCGAGATCAGG
AACGTGCCGCAGATCGAACGCCGGCTGGCCGAGGCCGACCGGCTGGGTTTCAGGAAGGCGCTCGTCCCGGTCAATGCCAG
GATCAAACCGCCGTCAACGGATTTCAAGCTGATCCCCGTCCGCGACATCCGCCAGGCGCTGCGGGAGGGCTTGACCGCCG
CCCGGGAAGAACCCGAGGCCGAAACCGGGCAGTGTCTTCTCTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-424)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-33)

AAA domain (AAA_25)

(75-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.976

98.715

0.503
  radA Streptococcus mitis SK321

50.22

97.216

0.488
  radA Streptococcus mitis NCTC 12261

50

97.216

0.486
  radA Streptococcus pneumoniae Rx1

50

97.216

0.486
  radA Streptococcus pneumoniae D39

50

97.216

0.486
  radA Streptococcus pneumoniae R6

50

97.216

0.486
  radA Streptococcus pneumoniae TIGR4

50

97.216

0.486

Multiple sequence alignment