Detailed information NTG1012377

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ABU996_RS14780
• Genome accession: NZ_CP159123
• Coordinates: 2814579..2815379 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola strain 10034763-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2809579..2820379

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABU996_RS14745 (ABU996_14745)	gspH	2809732..2810247 (+)	516	WP_080835748.1	type II secretion system minor pseudopilin GspH	-
ABU996_RS14750 (ABU996_14750)	gspI	2810244..2810609 (+)	366	WP_080835746.1	type II secretion system minor pseudopilin GspI	-
ABU996_RS14755 (ABU996_14755)	gspJ	2810606..2811202 (+)	597	WP_080835743.1	type II secretion system minor pseudopilin GspJ	-
ABU996_RS14760 (ABU996_14760)	gspK	2811199..2812179 (+)	981	WP_080835740.1	type II secretion system minor pseudopilin GspK	-
ABU996_RS14765 (ABU996_14765)	gspL	2812172..2813368 (+)	1197	WP_080835738.1	type II secretion system protein GspL	-
ABU996_RS14770 (ABU996_14770)	-	2813361..2813846 (+)	486	WP_012968921.1	type II secretion system protein M	-
ABU996_RS14775 (ABU996_14775)	-	2813836..2814582 (+)	747	WP_023322077.1	type II secretion system protein N	-
ABU996_RS14780 (ABU996_14780)	pilD	2814579..2815379 (+)	801	WP_023322076.1	A24 family peptidase	Machinery gene
ABU996_RS14785 (ABU996_14785)	hrpB	2815426..2817855 (-)	2430	WP_080835736.1	ATP-dependent helicase HrpB	-
ABU996_RS14790 (ABU996_14790)	thpR	2817928..2818464 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
ABU996_RS14795 (ABU996_14795)	sfsA	2818464..2819180 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
ABU996_RS14800 (ABU996_14800)	dksA	2819343..2819798 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=1012377 ABU996_RS14780 WP_023322076.1 2814579..2815379(+) (pilD) [Klebsiella variicola strain 10034763-2]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1012377 ABU996_RS14780 WP_023322076.1 2814579..2815379(+) (pilD) [Klebsiella variicola strain 10034763-2]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425