Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   V5233_RS00435 Genome accession   NZ_CP158667
Coordinates   90488..91837 (-) Length   449 a.a.
NCBI ID   WP_000815646.1    Uniprot ID   Q9XB96
Organism   Staphylococcus aureus strain BSN25     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 85488..96837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5233_RS00405 (V5233_0000405) - 85506..86591 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  V5233_RS00410 (V5233_0000410) - 86710..87027 (-) 318 WP_000659050.1 JAB domain-containing protein -
  V5233_RS00415 (V5233_0000415) - 87048..87554 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  V5233_RS00420 (V5233_0000420) - 87572..87883 (-) 312 WP_000700853.1 DUF960 family protein -
  V5233_RS00425 (V5233_0000425) - 87970..88320 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  V5233_RS00430 (V5233_0000430) ccrB 88838..90466 (-) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  V5233_RS00435 (V5233_0000435) ccrA 90488..91837 (-) 1350 WP_000815646.1 cassette chromosome recombinase CcrA Machinery gene
  V5233_RS00440 (V5233_0000440) cch1 92071..93864 (-) 1794 WP_000195105.1 cassette chromosome replicative helicase -
  V5233_RS00445 (V5233_0000445) ssb 93864..94160 (-) 297 WP_000273824.1 cassette chromosome ssDNA-binding protein -
  V5233_RS00450 (V5233_0000450) - 94353..95399 (-) 1047 WP_001060127.1 hypothetical protein -
  V5233_RS00455 (V5233_0000455) - 95650..96791 (-) 1142 Protein_88 IS3-like element ISSau4 family transposase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52607.20 Da        Isoelectric Point: 9.9585

>NTDB_id=1011843 V5233_RS00435 WP_000815646.1 90488..91837(-) (ccrA) [Staphylococcus aureus strain BSN25]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1011843 V5233_RS00435 WP_000815646.1 90488..91837(-) (ccrA) [Staphylococcus aureus strain BSN25]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterProScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

100

100

1
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment