Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABN702_RS04835 Genome accession   NZ_CP158164
Coordinates   998901..999968 (+) Length   355 a.a.
NCBI ID   WP_417899627.1    Uniprot ID   -
Organism   Bacillus sp. CSS-39     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 993901..1004968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABN702_RS04805 (ABN702_04785) - 994319..994591 (+) 273 WP_417899622.1 hypothetical protein -
  ABN702_RS04810 (ABN702_04790) - 994750..994833 (+) 84 WP_078379727.1 YjcZ family sporulation protein -
  ABN702_RS04815 (ABN702_04795) - 994897..995058 (-) 162 WP_417899623.1 hypothetical protein -
  ABN702_RS04820 (ABN702_04800) - 995366..996634 (+) 1269 WP_417899624.1 ATP-binding protein -
  ABN702_RS04825 (ABN702_04805) - 996852..997955 (+) 1104 WP_417899625.1 lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein -
  ABN702_RS04830 (ABN702_04810) - 998231..998746 (+) 516 WP_417899626.1 type 1 glutamine amidotransferase domain-containing protein -
  ABN702_RS04835 (ABN702_04815) comGA 998901..999968 (+) 1068 WP_417899627.1 competence type IV pilus ATPase ComGA Machinery gene
  ABN702_RS04840 (ABN702_04820) comGB 999952..1000989 (+) 1038 WP_417899628.1 competence type IV pilus assembly protein ComGB -
  ABN702_RS04845 (ABN702_04825) comGC 1000991..1001335 (+) 345 WP_417899629.1 competence type IV pilus major pilin ComGC -
  ABN702_RS04850 (ABN702_04830) comGD 1001316..1001774 (+) 459 WP_417900830.1 competence type IV pilus minor pilin ComGD -
  ABN702_RS04855 (ABN702_04835) - 1001758..1002099 (+) 342 WP_417899630.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABN702_RS04860 (ABN702_04840) comGF 1002083..1002523 (+) 441 WP_417899631.1 competence type IV pilus minor pilin ComGF -
  ABN702_RS04865 (ABN702_04845) comGG 1002516..1002893 (+) 378 WP_417899632.1 competence type IV pilus minor pilin ComGG -
  ABN702_RS04870 (ABN702_04850) - 1002969..1003193 (+) 225 WP_417899633.1 YuzF family protein -
  ABN702_RS04875 (ABN702_04855) - 1003309..1003824 (+) 516 WP_417899634.1 shikimate kinase -
  ABN702_RS04880 (ABN702_04860) - 1003857..1004039 (+) 183 WP_204415635.1 YqzE family protein -
  ABN702_RS04885 (ABN702_04865) - 1004489..1004755 (+) 267 WP_204415634.1 phosphocarrier protein HPr -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40475.05 Da        Isoelectric Point: 7.7267

>NTDB_id=1010024 ABN702_RS04835 WP_417899627.1 998901..999968(+) (comGA) [Bacillus sp. CSS-39]
MNIEQKCEDLVRQALKLRVSDIHLKPLESSATLMFRVDHYLYEQEEISLEIYERMVSHLKFQASMDIGETRIPQNGALTL
TIDTKQINLRLSTLPTLYNESVVIRILPQDSHQPLKYMTLFPNSTTKLLSLMKYSHGLIIFTGPTGSGKTTTLYSLIEET
KSLLQRNIITLEDPVERKSKNVLQVQVNEKAGITYTTGLKAILRHDPDIIMVGEIRDEETAKIAIRASMTGHLVLSTLHT
RNAKGAVYRLLEFGVTPQELEQTLIAISAQRLVELKCPFCLEECTAFCRKYRTHRLASVHELLYGKELGKVMKECNGAEV
DLTYPTLREVIKKGIALGFIKQSEYERWVFDGMEK

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=1010024 ABN702_RS04835 WP_417899627.1 998901..999968(+) (comGA) [Bacillus sp. CSS-39]
ATGAATATCGAACAAAAGTGTGAAGACCTCGTTCGCCAAGCGCTAAAGCTTCGCGTTTCAGACATCCACTTGAAGCCTTT
GGAATCTTCCGCAACCCTCATGTTCAGGGTAGACCATTACCTTTATGAGCAGGAGGAAATTTCACTCGAGATCTATGAAA
GAATGGTCTCCCATCTTAAATTCCAAGCCTCCATGGACATAGGAGAAACGAGGATACCGCAAAATGGCGCATTAACGCTC
ACCATTGATACCAAACAAATCAACCTCAGACTCTCGACCTTACCTACCCTTTATAACGAAAGTGTAGTCATTCGCATCCT
CCCACAGGATTCACATCAACCATTGAAATACATGACCCTATTTCCCAACTCCACCACCAAGCTACTTTCCTTAATGAAAT
ACTCCCATGGATTGATCATTTTTACGGGTCCTACTGGTTCGGGCAAGACCACCACCCTATACTCCTTAATTGAAGAAACG
AAATCCCTTTTACAGCGGAATATCATTACCCTTGAAGATCCTGTGGAACGAAAAAGTAAGAATGTTTTGCAGGTTCAGGT
GAATGAAAAGGCCGGAATTACGTATACCACCGGATTAAAAGCGATACTCAGACATGACCCCGATATTATAATGGTCGGGG
AAATCCGGGACGAGGAAACGGCAAAGATTGCAATAAGGGCCTCCATGACCGGACATCTTGTATTGAGCACCTTGCATACG
AGAAATGCAAAAGGGGCGGTATATCGGCTATTGGAATTCGGCGTCACCCCACAAGAGCTGGAACAGACGCTGATTGCCAT
TTCTGCGCAGAGGCTGGTAGAACTGAAGTGTCCATTTTGTCTAGAGGAATGCACAGCATTCTGCAGAAAATATCGCACCC
ACCGTCTCGCAAGTGTTCATGAGCTGCTCTATGGGAAAGAGCTTGGAAAGGTCATGAAGGAATGTAATGGGGCGGAAGTG
GATTTGACGTACCCGACGCTTAGAGAAGTGATAAAAAAAGGGATAGCTCTTGGGTTTATCAAACAAAGCGAGTATGAGCG
GTGGGTCTTTGATGGAATGGAGAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56

98.592

0.552
  ctsE Campylobacter jejuni subsp. jejuni 81-176

37.26

100

0.383
  pilB Glaesserella parasuis strain SC1401

36.975

100

0.372

Multiple sequence alignment