Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   P303_RS03370 Genome accession   NZ_CP006740
Coordinates   743758..745458 (+) Length   566 a.a.
NCBI ID   WP_020852927.1    Uniprot ID   -
Organism   Xylella fastidiosa MUL0034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738758..750458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P303_RS13910 (P303_03590) - 738875..739393 (+) 519 WP_228304192.1 hypothetical protein -
  P303_RS13915 (P303_03595) - 739427..739786 (+) 360 WP_004084115.1 AEC family transporter -
  P303_RS03365 (P303_03600) - 740451..743465 (+) 3015 WP_040123129.1 YadA-like family protein -
  P303_RS03370 (P303_03605) pilF 743758..745458 (+) 1701 WP_020852927.1 type II secretion system ATPase GspE Machinery gene
  P303_RS03375 (P303_03610) - 745494..746711 (+) 1218 WP_038229123.1 type II secretion system F family protein -
  P303_RS03380 (P303_03620) gspG 746929..747363 (+) 435 WP_004088955.1 type II secretion system major pseudopilin GspG -
  P303_RS03385 (P303_03625) - 747369..747875 (+) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  P303_RS03390 (P303_03630) - 747872..748270 (+) 399 WP_004088953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P303_RS03395 (P303_03635) - 748267..748893 (+) 627 WP_011097763.1 type II secretion system protein J -
  P303_RS03400 (P303_03640) - 748893..749738 (+) 846 WP_040123130.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62683.50 Da        Isoelectric Point: 5.0845

>NTDB_id=100996 P303_RS03370 WP_020852927.1 743758..745458(+) (pilF) [Xylella fastidiosa MUL0034]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPMHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=100996 P303_RS03370 WP_020852927.1 743758..745458(+) (pilF) [Xylella fastidiosa MUL0034]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGCATCAATCAGATCCAGGCGAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCGGCGCTGACAGGTCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTATGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATCGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGCTTGCGCCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAATTTCTGATTATGGATGATGCGCTGCGTCGCGCTGTGATCAGCCGGACCGGCATAGGGGAGTTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.761

97.527

0.398
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

40.074

95.23

0.382
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369

Multiple sequence alignment