Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D934_RS09780 Genome accession   NZ_CP006696
Coordinates   2092089..2093789 (-) Length   566 a.a.
NCBI ID   WP_020852927.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. sandyi Ann-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2087089..2098789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D934_RS09750 (D934_10450) - 2087675..2088520 (-) 846 WP_020852923.1 type II secretion system protein GspK -
  D934_RS09755 (D934_10455) - 2088520..2089146 (-) 627 WP_020852924.1 type II secretion system protein J -
  D934_RS09760 (D934_10460) - 2089143..2089541 (-) 399 WP_020852925.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D934_RS09765 (D934_10465) - 2089538..2090044 (-) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  D934_RS09770 (D934_10470) gspG 2090050..2090484 (-) 435 WP_004088955.1 type II secretion system major pseudopilin GspG -
  D934_RS09775 (D934_10485) - 2090836..2092053 (-) 1218 WP_020852926.1 type II secretion system F family protein -
  D934_RS09780 (D934_10490) pilF 2092089..2093789 (-) 1701 WP_020852927.1 type II secretion system ATPase GspE Machinery gene
  D934_RS09785 (D934_10495) - 2094084..2097095 (-) 3012 WP_024748763.1 YadA-like family protein -
  D934_RS09790 (D934_10500) - 2097769..2098680 (-) 912 WP_024748764.1 AEC family transporter -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62683.50 Da        Isoelectric Point: 5.0845

>NTDB_id=100957 D934_RS09780 WP_020852927.1 2092089..2093789(-) (pilF) [Xylella fastidiosa subsp. sandyi Ann-1]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPMHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=100957 D934_RS09780 WP_020852927.1 2092089..2093789(-) (pilF) [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGTGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGCATCAATCAGATCCAGGCGAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACGGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTATGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATCGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGTTGGCACCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAATTTCTGATTATGGATGATGCGCTGCGCCGCGCTGTGATCAGCCGGACCGGCATAGGGGAGCTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)

Type II/IV secretion system protein (T2SSE)

(189-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.761

97.527

0.398
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

40.074

95.23

0.382
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369