Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUD79_RS05755 Genome accession   NZ_AP027362
Coordinates   1329088..1330212 (+) Length   374 a.a.
NCBI ID   WP_184425716.1    Uniprot ID   A0A7X0NJC4
Organism   Thalassotalea piscium strain JCM 18590     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1324088..1335212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD79_RS05725 - 1324753..1324989 (+) 237 WP_286290776.1 EamA family transporter -
  QUD79_RS05730 - 1325018..1325458 (-) 441 WP_184425704.1 DUF4426 domain-containing protein -
  QUD79_RS05735 - 1325520..1326068 (-) 549 WP_184425706.1 YggT family protein -
  QUD79_RS05740 proC 1326105..1326920 (-) 816 WP_184425708.1 pyrroline-5-carboxylate reductase -
  QUD79_RS05745 - 1327095..1327766 (-) 672 WP_184425711.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUD79_RS05750 pilT 1327826..1328869 (+) 1044 WP_184425714.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD79_RS05755 pilU 1329088..1330212 (+) 1125 WP_184425716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUD79_RS05760 - 1330290..1331975 (-) 1686 WP_184425718.1 pseudouridine synthase -
  QUD79_RS05765 - 1331980..1332939 (-) 960 WP_184425720.1 AEC family transporter -
  QUD79_RS05770 ruvX 1333001..1333447 (-) 447 WP_184425722.1 Holliday junction resolvase RuvX -
  QUD79_RS05775 - 1333447..1334001 (-) 555 WP_184425724.1 YqgE/AlgH family protein -
  QUD79_RS05780 gshB 1334060..1335010 (-) 951 WP_184425726.1 glutathione synthase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41136.09 Da        Isoelectric Point: 5.5852

>NTDB_id=100810 QUD79_RS05755 WP_184425716.1 1329088..1330212(+) (pilU) [Thalassotalea piscium strain JCM 18590]
MRFHNLLVKMVQEDASDMFVTAKLPVSAKINGELQPIDSQVLTADEALGLVHDAMNEKQKKQFDEEKECNFAISIDDIGR
FRVSAFWQRDMAGMVVRRIVTEIPSADDLGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNKNSRGHILTIED
PVEFVHEHGKSMITQREVGLDTESFDAALQSSLRQAPDVILIGEIRSKEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPSDQHGKLLFDLALNLRGIIAQQLIPTRDGNGRVAAIEILLNSPFIGELIKKGDISSIKEVMEKSTEQGMQTFDQA
LFTLYQRGLINYADALHHADSPNDLRLMIKLRSNDQGGSGSLAGVTLDGLEPKE

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=100810 QUD79_RS05755 WP_184425716.1 1329088..1330212(+) (pilU) [Thalassotalea piscium strain JCM 18590]
ATGAGATTTCATAATTTACTAGTTAAAATGGTGCAAGAAGATGCATCCGACATGTTTGTAACAGCTAAATTACCTGTTAG
CGCAAAAATAAATGGCGAGTTACAACCCATCGATTCACAAGTGCTAACCGCAGATGAAGCGTTAGGTTTAGTGCATGATG
CAATGAATGAAAAGCAAAAGAAGCAATTCGACGAAGAGAAAGAGTGTAACTTTGCAATTTCTATTGATGACATTGGTCGA
TTTCGTGTATCCGCATTTTGGCAAAGAGATATGGCTGGCATGGTTGTACGTCGTATTGTTACCGAAATACCAAGTGCCGA
CGATTTAGGGCTACCCTCAGTTCTAAAAGACGTTGTAATGTCTAAACGTGGTTTAGTGCTATTTGTAGGTGGTACCGGCA
CCGGTAAATCAACCTCGATGGCAGCGTTAATTGGTTATCGAAATAAAAACTCTCGTGGGCATATTCTTACTATTGAAGAC
CCAGTTGAATTTGTACATGAACATGGTAAGTCGATGATTACCCAACGCGAAGTTGGGCTAGATACCGAATCGTTTGATGC
CGCATTACAAAGCTCGTTACGACAAGCACCTGATGTTATTTTAATTGGAGAGATCCGTTCAAAAGAAATAATGGAACATG
CATTAAGTTTCGCGGAAACAGGGCATTTGTGTATTGCTACCTTGCATGCCAATAACGCAAACCAAGCGATTGACCGGATA
ATGCACTTAGTGCCTTCAGATCAACATGGTAAATTGTTATTTGATTTAGCATTAAACTTACGCGGCATTATTGCTCAACA
ACTTATTCCTACCCGCGATGGCAATGGCCGAGTTGCAGCAATTGAGATTTTATTAAACTCGCCGTTTATTGGTGAATTAA
TTAAAAAAGGTGATATTAGTAGCATTAAAGAAGTTATGGAAAAGTCTACTGAACAAGGAATGCAAACCTTTGATCAGGCT
TTATTTACTTTATACCAACGCGGTTTAATTAACTATGCAGATGCATTGCATCATGCTGATTCACCTAACGATTTACGCTT
AATGATTAAATTGCGCAGTAATGACCAAGGCGGCTCTGGCTCGCTAGCGGGTGTAACCTTAGATGGGTTAGAGCCTAAAG
AATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0NJC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.664

95.455

0.57
  pilU Acinetobacter baylyi ADP1

58.523

94.118

0.551
  pilU Vibrio cholerae strain A1552

54.076

98.396

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.458

95.722

0.406
  pilT Pseudomonas aeruginosa PAK

39.71

92.246

0.366
  pilT Acinetobacter baylyi ADP1

40.058

91.444

0.366
  pilT Acinetobacter nosocomialis M2

39.766

91.444

0.364
  pilT Acinetobacter baumannii D1279779

39.766

91.444

0.364
  pilT Acinetobacter baumannii strain A118

39.766

91.444

0.364

Multiple sequence alignment