Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUD79_RS05750 Genome accession   NZ_AP027362
Coordinates   1327826..1328869 (+) Length   347 a.a.
NCBI ID   WP_184425714.1    Uniprot ID   A0A7X0NJG8
Organism   Thalassotalea piscium strain JCM 18590     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1322826..1333869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD79_RS05715 - 1323363..1323977 (-) 615 WP_246455009.1 XTP/dITP diphosphatase -
  QUD79_RS05720 - 1324085..1324696 (+) 612 WP_246455010.1 EamA family transporter -
  QUD79_RS05725 - 1324753..1324989 (+) 237 WP_286290776.1 EamA family transporter -
  QUD79_RS05730 - 1325018..1325458 (-) 441 WP_184425704.1 DUF4426 domain-containing protein -
  QUD79_RS05735 - 1325520..1326068 (-) 549 WP_184425706.1 YggT family protein -
  QUD79_RS05740 proC 1326105..1326920 (-) 816 WP_184425708.1 pyrroline-5-carboxylate reductase -
  QUD79_RS05745 - 1327095..1327766 (-) 672 WP_184425711.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUD79_RS05750 pilT 1327826..1328869 (+) 1044 WP_184425714.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD79_RS05755 pilU 1329088..1330212 (+) 1125 WP_184425716.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUD79_RS05760 - 1330290..1331975 (-) 1686 WP_184425718.1 pseudouridine synthase -
  QUD79_RS05765 - 1331980..1332939 (-) 960 WP_184425720.1 AEC family transporter -
  QUD79_RS05770 ruvX 1333001..1333447 (-) 447 WP_184425722.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38774.30 Da        Isoelectric Point: 6.0548

>NTDB_id=100809 QUD79_RS05750 WP_184425714.1 1327826..1328869(+) (pilT) [Thalassotalea piscium strain JCM 18590]
MDITELLAFSVENNASDLHLSTGIPPSIRVDGDVRKLNIPAFDAKDVNGLVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPSAVFRTIPSKVLSLEELGCPDIFRDISDTPRGLVLVTGPTGSGKSTTLAAMVDYINKNKYHHILTIED
PIEFVHENKSCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLVKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLVNRGMISRQDAMEKAADKNQFKTY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=100809 QUD79_RS05750 WP_184425714.1 1327826..1328869(+) (pilT) [Thalassotalea piscium strain JCM 18590]
ATGGATATTACCGAATTACTCGCGTTTAGCGTAGAAAATAATGCATCTGATTTACATCTTTCTACCGGCATTCCTCCTTC
AATTCGCGTTGATGGTGATGTTAGAAAATTAAATATTCCAGCGTTTGACGCTAAAGATGTTAATGGTCTTGTTTATGACA
TTATGAATGATAGACAGCGAAAAGAGTACGAAGAAAACTTAGAGGTCGATTTTTCATTCGAAGTACCAAATTTAGCACGT
TTTAGGGTGAATGCGTTTAATCAAAACCGTGGGCCGTCGGCAGTATTTCGTACCATTCCTAGTAAAGTGCTTTCGCTTGA
AGAGCTTGGTTGCCCTGATATTTTTAGAGATATTTCTGACACCCCTCGTGGTTTAGTGCTTGTTACAGGCCCTACTGGTT
CGGGTAAATCAACTACGCTAGCGGCTATGGTTGACTATATTAACAAAAATAAATATCACCATATTTTAACCATTGAAGAC
CCGATAGAATTTGTTCATGAAAATAAATCGTGTTTAATTAACCAACGGGAAGTACACCGTGATACATTAAGTTTTAATAA
TGCGCTACGTTCTGCGCTTCGTGAAGACCCTGATGTTATTCTTGTGGGTGAAATGCGAGATTTAGAAACCATTCGTTTAG
CAATGACAGCAGCAGAAACGGGCCATTTAGTCTTTGGTACCTTGCATACAACTTCAGCACCAAAAACAATAGACCGTATT
ATTGATGTGTTTCCAGCCGAAGAAAAGTCTATGGTGCGTTCAATGTTATCTGAATCATTACGTGCGGTAATTTCACAAAC
GCTAGTTAAAAAAGTTGGCGGCGGACGCGTAGCTGCGCATGAAATTATGATAGGTGTTCCAGCTATTCGTAACTTAATTC
GTGAAGACAAAATTGCACAAATGTATTCAGCTATTCAAACGGGTATGTCGCATGGTATGCAAACAATGGATCAATGTTTA
CAAAACTTGGTTAATCGCGGCATGATTTCGCGCCAAGATGCGATGGAAAAAGCGGCAGATAAAAATCAATTTAAAACATA
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0NJG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.135

0.781
  pilT Acinetobacter nosocomialis M2

78.488

99.135

0.778
  pilT Acinetobacter baumannii D1279779

78.488

99.135

0.778
  pilT Acinetobacter baumannii strain A118

78.488

99.135

0.778
  pilT Acinetobacter baylyi ADP1

78.198

99.135

0.775
  pilT Pseudomonas aeruginosa PAK

77.907

99.135

0.772
  pilT Legionella pneumophila strain Lp02

73.837

99.135

0.732
  pilT Legionella pneumophila strain ERS1305867

73.837

99.135

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.118

0.7
  pilT Vibrio cholerae strain A1552

72.107

97.118

0.7
  pilT Neisseria meningitidis 8013

69.795

98.271

0.686
  pilT Neisseria gonorrhoeae MS11

69.501

98.271

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

99.424

0.53
  pilU Pseudomonas stutzeri DSM 10701

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

41.618

99.712

0.415
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395

Multiple sequence alignment