Detailed information    

experimental Experimentally validated

Overview

Name   comC   Type   Machinery gene
Locus tag   ACIAD_RS15010 Genome accession   NC_005966
Coordinates   3220340..3224692 (-) Length   1450 a.a.
NCBI ID   WP_004923840.1    Uniprot ID   A0A7G2SAG8
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

ComC is localized at the cell periphery, probably at the tip of the DNA-transporting structure, and involved in binding and transport of DNA


Genomic Context

Location: 3215340..3229692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS14975 (ACIAD3309) - 3216014..3216982 (+) 969 WP_004923853.1 esterase/lipase family protein -
  ACIAD_RS14980 (ACIAD3310) rplS 3217052..3217423 (-) 372 WP_004923849.1 50S ribosomal protein L19 -
  ACIAD_RS14985 (ACIAD3311) trmD 3217565..3218314 (-) 750 WP_004923847.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACIAD_RS14990 (ACIAD3312) rimM 3218362..3218910 (-) 549 WP_004923846.1 ribosome maturation factor RimM -
  ACIAD_RS14995 (ACIAD3313) rpsP 3218930..3219187 (-) 258 WP_004923845.1 30S ribosomal protein S16 -
  ACIAD_RS15000 (ACIAD3314) comF 3219335..3219769 (-) 435 WP_004923844.1 type IV pilin protein Machinery gene
  ACIAD_RS15005 (ACIAD3315) comE 3219769..3220278 (-) 510 WP_004923843.1 type IV pilin protein Machinery gene
  ACIAD_RS15010 (ACIAD3316) comC 3220340..3224692 (-) 4353 WP_004923840.1 PilC/PilY family type IV pilus protein Machinery gene
  ACIAD_RS15015 (ACIAD3317) pilX 3224709..3225377 (-) 669 WP_004923837.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACIAD_RS15020 (ACIAD3318) comB 3225374..3226342 (-) 969 WP_004923834.1 PilW family protein Machinery gene
  ACIAD_RS15025 (ACIAD3319) pilV 3226343..3226879 (-) 537 WP_004923832.1 type IV pilus modification protein PilV Machinery gene
  ACIAD_RS15030 (ACIAD3321) - 3226876..3227322 (-) 447 WP_004923829.1 GspH/FimT family pseudopilin -
  ACIAD_RS15035 (ACIAD3322) ispH 3227449..3228399 (-) 951 WP_004923824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACIAD_RS15040 (ACIAD3324) gmk 3228534..3229157 (+) 624 WP_004923822.1 guanylate kinase -
  ACIAD_RS15045 (ACIAD3325) rpoZ 3229226..3229507 (+) 282 WP_004923811.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 1450 a.a.        Molecular weight: 157837.56 Da        Isoelectric Point: 6.3258

>NTDB_id=1008 ACIAD_RS15010 WP_004923840.1 3220340..3224692(-) (comC) [Acinetobacter baylyi ADP1]
MKKSIYTQYLNSLYLVLQHKFSIFSISVFSCMLIMTSVVQASDVEVYQQGAQFDKRLMLMVDQSRSMGGAGALDLLKEYP
ICVGKGVSNVLGSGGGLGILGDKDALELVTDTVGAVDQVVLQGLLGGSLDPLLKITTESPSSKYNYARNYCTVVTTDLVV
KTLDGLLKPLLGATGLDAKSYIENTCDFQANIQLLNSVSVVGVYRCYDKLSRVKNALTDVLLGNDKTGLKPLPDNVSVGL
SAMPVDIMTKDRAGRILAPACKLSTTKASDTSNQCFEKGYAGSATTYRGYLVEKVANGIQSKGEFNLQNILLIVPKLVAA
LVGDVFNKLIGILTNPIRGLTDLLEFENTANVINDLLKTLGLSGNIPTASTYAETGAYLLGTSTKGTGARQIAMRIIPVK
ILFIPLLGWYDYKCDKYGKDSWQADGVTCKDDAWQYKLGGISTTNLIQVNDDLVSSLISDGVVGGLLNGVVGNILGIKVS
EKHLYYGYDSQADIEYSGFNYRDNSVPTRNNNQFYQAPANQPQCNASGIMVITGGVPNITPTTTDVLLQPSGKEGLGTKN
AIERLMGRSLDTQATDLSRLDLQSVFQCDESAGLKSTTLSSRDYATWSCIGNYSKKLLDKNVSTGVIGVGREFITIPSTK
DSAQLEASMSNLNNSLLGQTVPNLVKDTLNVLLGGPLVNGLTNLLGNLFPTDAEDVKNLARWGVLGKGGWYNSASSENIA
NSVYSFYSDLGVTKRETFLGAPVIPTDPLTPYNLNNYVFQNMFVPDDKQTWFGNVKKYMTDTGETIQTKTFQDVWSNQNI
DQTNILSGGVIDKLPIASGNNASTRQLYINRTCDTKAKKYDVSNAISAVGNNYYTQLCAGQTTDPRRNDLMNLLGYQIQS
VNNQETLVAKPEYRKIGMVLHSTPIKITQSATVKDDGSLTRDDYLVFGSAEGLLHVVNADTGVEKLAFLPNEMLENSKQR
KAFTGNGLEGHDSFSNMQYGVDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMGGRSYYAL
DLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQGKRKLVMFVGGGYDAGGTDGNSNNGGY
ELANYNQTNKRGAGVYMFDAENGDLLWWANNLATTSNDVSNSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLW
RVDINNRVDAKQFASAALLLDLQSNRPTDLKDTNVRFYEAPVFSIYGYGSESLAVLSIASSNRSLPISDKSAGAIFNIFD
KDVTQVSFTTRDTYTDNKNLVAYSQLPKWGALTNENKPQYGWYVKLIDQQKVMDETAVINKSLYVSIYDPIAQDGRVADC
SIGIQGLSSIRRYCLPYGVCEKQTDLSGMLKLGKGILPVTIGSGSTDNKSTRQLIGGFSKDERTNAANVLGQNTLRRQIV
PLKWYEQSTP

Nucleotide


Download         Length: 4353 bp        

>NTDB_id=1008 ACIAD_RS15010 WP_004923840.1 3220340..3224692(-) (comC) [Acinetobacter baylyi ADP1]
ATGAAAAAATCGATATACACTCAGTATCTCAACTCTCTTTATTTGGTCTTGCAGCATAAGTTCAGTATTTTCAGTATTTC
AGTATTTAGCTGTATGCTAATCATGACTTCTGTTGTGCAGGCAAGCGATGTAGAGGTTTACCAGCAGGGAGCGCAGTTTG
ATAAACGACTGATGCTTATGGTCGATCAGTCGCGAAGCATGGGAGGAGCTGGAGCACTTGATCTATTAAAAGAATATCCA
ATTTGTGTGGGCAAAGGGGTATCCAATGTTTTGGGCTCTGGTGGCGGGTTAGGCATACTCGGCGACAAAGACGCGCTGGA
ATTGGTGACCGATACAGTAGGTGCGGTCGATCAGGTTGTTTTACAAGGCTTACTGGGTGGAAGTCTTGATCCGCTTTTAA
AAATCACTACTGAATCACCCAGTAGTAAATATAACTATGCACGTAATTACTGTACTGTTGTTACAACCGATTTGGTAGTG
AAAACATTAGATGGTTTATTAAAACCTTTACTTGGGGCAACAGGGTTAGATGCAAAAAGTTATATTGAAAATACTTGCGA
CTTTCAGGCGAATATCCAATTATTAAACAGTGTTTCTGTTGTAGGCGTTTATCGTTGCTATGATAAATTGTCCCGTGTAA
AAAATGCTTTGACAGATGTGTTGCTGGGGAATGATAAAACTGGCTTAAAACCTTTGCCAGATAATGTCAGTGTTGGATTA
TCAGCGATGCCTGTAGATATCATGACTAAAGATCGAGCAGGTCGGATTCTGGCACCAGCATGTAAGTTATCTACAACAAA
AGCCAGCGATACATCAAATCAATGTTTTGAAAAGGGATATGCGGGTTCGGCAACGACCTATCGTGGTTATTTAGTGGAAA
AAGTTGCTAATGGTATTCAATCTAAAGGTGAATTTAATCTTCAGAATATATTGTTGATTGTTCCCAAGCTAGTTGCTGCA
TTGGTAGGGGATGTATTCAACAAGCTAATAGGAATTCTTACCAATCCAATTCGAGGGTTAACAGATCTTCTTGAATTTGA
AAACACAGCAAATGTTATTAATGACTTGTTAAAAACACTGGGTTTATCTGGGAATATTCCAACAGCAAGTACCTACGCAG
AAACGGGGGCGTACCTGCTTGGAACATCGACCAAGGGTACAGGCGCTAGACAAATAGCAATGAGAATTATTCCAGTAAAA
ATTCTATTTATTCCGCTGTTAGGCTGGTACGATTATAAATGTGATAAATATGGAAAAGACAGCTGGCAAGCTGATGGTGT
GACGTGTAAGGATGATGCCTGGCAGTATAAGCTTGGTGGAATATCAACAACCAATCTTATACAGGTAAATGATGATCTGG
TCAGCTCCCTTATCAGTGATGGTGTGGTGGGGGGACTCTTAAATGGTGTGGTTGGTAACATCTTGGGAATCAAGGTCTCA
GAGAAACATCTTTACTATGGCTATGATTCACAAGCCGATATTGAATATAGTGGTTTTAATTATCGAGATAATTCTGTTCC
AACCAGAAATAATAATCAGTTTTATCAGGCACCAGCAAATCAGCCACAATGTAATGCCAGCGGAATTATGGTGATTACTG
GAGGTGTTCCAAATATTACCCCAACTACAACGGATGTTTTACTACAACCATCAGGTAAAGAAGGTTTGGGAACAAAAAAT
GCAATAGAGCGTTTAATGGGACGTTCATTGGATACCCAAGCAACAGATCTTTCCAGATTGGATCTGCAAAGTGTCTTTCA
ATGCGATGAGAGTGCTGGTTTAAAATCTACAACTCTCAGTAGTCGCGATTATGCTACATGGAGTTGTATTGGAAATTACA
GCAAAAAATTGCTGGATAAAAATGTGTCCACTGGCGTGATTGGAGTGGGGCGGGAGTTTATTACCATTCCGAGCACAAAA
GATTCTGCACAACTTGAAGCATCAATGAGTAACTTGAATAATTCACTATTAGGACAGACTGTTCCTAATCTAGTTAAAGA
TACTTTGAATGTGCTTTTGGGTGGACCATTGGTGAATGGATTGACTAACTTACTTGGAAATTTATTTCCAACAGATGCAG
AAGATGTTAAAAATTTGGCACGTTGGGGTGTGCTGGGCAAGGGTGGATGGTACAACTCTGCAAGTTCAGAGAATATTGCG
AATAGTGTTTATTCATTTTACTCAGATTTAGGGGTTACGAAACGAGAGACTTTCTTGGGAGCACCAGTAATTCCAACAGA
TCCTTTAACACCATACAATTTAAATAATTACGTTTTTCAAAATATGTTTGTTCCAGATGATAAACAAACATGGTTTGGTA
ACGTTAAAAAATATATGACAGATACTGGTGAAACAATCCAGACTAAAACTTTTCAAGATGTCTGGAGTAATCAGAATATT
GATCAGACTAATATTTTGAGTGGTGGCGTGATTGATAAATTACCTATTGCTTCTGGCAACAACGCATCTACGCGTCAGCT
TTATATCAATCGGACTTGTGATACAAAAGCCAAAAAATACGATGTCTCTAATGCGATTAGTGCAGTTGGTAATAATTACT
ATACTCAGCTCTGTGCAGGCCAGACTACGGATCCGCGCCGTAATGATCTGATGAATCTGTTGGGCTACCAGATTCAAAGT
GTCAATAATCAGGAAACTTTAGTTGCCAAGCCTGAGTACCGCAAGATTGGTATGGTTTTACATTCAACACCTATCAAAAT
CACACAGTCTGCCACAGTTAAGGATGATGGTTCTTTGACACGAGATGACTATCTTGTATTCGGTTCAGCAGAAGGTCTAT
TACACGTTGTAAATGCAGATACAGGCGTAGAAAAGCTAGCATTTTTACCAAATGAAATGCTGGAAAATTCTAAACAACGT
AAAGCATTTACAGGCAATGGTCTTGAAGGCCATGATAGTTTTAGTAACATGCAATATGGTGTAGATGGGCCATGGACTGC
CTATACCGAATATGTCTGGAATAGCAAAGACAATAAATTAACTGTAGGTAAATCTACTACTGATGCTGTTTGTATTAAAG
ATGGTGTATTTACAGGAGCATGTGGAAAACAATATCTTTATGGTGGTTTGCGTATGGGAGGGCGTAGTTATTATGCGCTT
GATCTGAATGATCTTGCTGCACCAAAACTCAAATTCTATATCGATCCAGCAAGTGGTCGGGTTTATTCCGATGCCTATCC
TGGTGGCAAAAGTTTTGATGCAATTAAAAATATGGGACAAAGTTGGTCAAAACCGACCATTGCTTGGATTAACTGGCAAG
GCAAACGTAAGTTGGTTATGTTTGTAGGTGGTGGCTACGACGCTGGCGGTACAGATGGCAATAGTAATAACGGTGGCTAT
GAACTTGCGAATTATAATCAGACCAATAAGCGTGGTGCAGGCGTTTACATGTTTGATGCCGAAAATGGTGATTTGCTTTG
GTGGGCAAATAATTTGGCAACGACATCAAATGACGTTTCGAATAGTGCCTTAAATACAAATATGCAATTCAGTGTTGTCG
GTAGAATCAATACAGTAGATCGAGATGGAGATGGTTTAATAGATCATCTTTACTTTGGTGATTTGGGGGGGCAGTTATGG
CGAGTCGATATTAATAATCGTGTAGATGCCAAGCAGTTTGCTTCGGCTGCGCTTCTTCTCGATTTACAATCTAACAGACC
TACAGATCTTAAAGATACAAATGTCCGTTTCTATGAAGCACCTGTATTTAGTATTTATGGTTATGGTAGTGAGTCGCTTG
CAGTTTTGAGCATTGCTTCAAGCAATCGTAGTTTGCCAATTAGTGATAAAAGTGCGGGTGCTATTTTTAATATCTTTGAC
AAGGATGTAACTCAAGTCAGTTTCACCACACGGGATACCTATACAGATAATAAAAATCTGGTTGCATATTCTCAATTACC
TAAATGGGGGGCTTTGACCAATGAAAATAAACCGCAATATGGTTGGTATGTGAAACTCATTGATCAGCAAAAAGTAATGG
ATGAAACAGCAGTAATCAATAAGAGTTTGTATGTCAGTATCTATGATCCGATTGCTCAGGATGGGCGTGTTGCAGATTGC
TCAATTGGTATACAAGGCTTAAGTAGCATCCGTCGTTATTGTTTACCTTATGGCGTATGTGAAAAACAGACCGATTTATC
AGGAATGCTAAAACTCGGAAAAGGGATTTTACCTGTAACAATTGGCTCAGGCTCAACGGATAATAAATCGACGAGACAAT
TGATCGGTGGATTTAGCAAAGACGAGCGTACCAACGCTGCCAACGTACTTGGGCAAAATACCCTACGCCGTCAGATCGTA
CCATTAAAATGGTATGAGCAAAGCACCCCTTAA

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(904-1209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]
[3] C Link et al. (1998) Identification and characterization of a novel competence gene, comC, required for DNA binding and uptake in Acinetobacter sp. strain BD413. Journal of Bacteriology 180(6):1592-5. [PMID: 9515934]