Detailed information    

experimental Experimentally validated

Overview

Name   comB   Type   Machinery gene
Locus tag   ACIAD_RS15020 Genome accession   NC_005966
Coordinates   3225374..3226342 (-) Length   322 a.a.
NCBI ID   WP_004923834.1    Uniprot ID   Q9RBI7
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The seven competence proteins, ComB, ComP, PilV, PilX and FimT are absolutely essential for natural transformation. These proteins are hydrophilic and are similar to pilins, the structural subunits of type IV pili (T4P). The disruption of comB, comP and the deletion of pilV, pilX or fimT resulted in complete transformation deficiencies.


Genomic Context

Location: 3220374..3231342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS15015 (ACIAD3317) pilX 3224709..3225377 (-) 669 WP_004923837.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACIAD_RS15020 (ACIAD3318) comB 3225374..3226342 (-) 969 WP_004923834.1 PilW family protein Machinery gene
  ACIAD_RS15025 (ACIAD3319) pilV 3226343..3226879 (-) 537 WP_004923832.1 type IV pilus modification protein PilV Machinery gene
  ACIAD_RS15030 (ACIAD3321) - 3226876..3227322 (-) 447 WP_004923829.1 GspH/FimT family pseudopilin -
  ACIAD_RS15035 (ACIAD3322) ispH 3227449..3228399 (-) 951 WP_004923824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACIAD_RS15040 (ACIAD3324) gmk 3228534..3229157 (+) 624 WP_004923822.1 guanylate kinase -
  ACIAD_RS15045 (ACIAD3325) rpoZ 3229226..3229507 (+) 282 WP_004923811.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35168.79 Da        Isoelectric Point: 8.7450

>NTDB_id=1001 ACIAD_RS15020 WP_004923834.1 3225374..3226342(-) (comB) [Acinetobacter baylyi ADP1]
MKISRHSGFTLVELMVAITLGLLVTAAAIQLYITGITSYNVQKAMSSIQDSASFGVNYILDDVRRANLSAPSAYMNDQQP
NSGLILTSSNVSELKDNKPLLTLNDAYLTKSAVGDGNVSQKSDQLTIRYQAVEPGYDCTGKTLPANTYVVQRYFIGSDKA
LRCVSDQYQQTGNTITGMDNKTKTTLDLSGNGQIIIPNVDYMRVQLGYVKGALNNPTAMSYVSIGDYLDTNKTSAVKKNI
NGIQQSIKPNINVIQIGLLVQSNETAGNDSIVRNRNKQAFDVLGQSVTLNEKNQDTRLRQVVTQTISLRNAMGWVEEGYV
TP

Nucleotide


Download         Length: 969 bp        

>NTDB_id=1001 ACIAD_RS15020 WP_004923834.1 3225374..3226342(-) (comB) [Acinetobacter baylyi ADP1]
ATGAAAATCTCTCGCCATTCAGGATTTACGCTAGTTGAACTCATGGTGGCCATTACATTGGGTTTGCTTGTTACAGCCGC
TGCAATTCAGCTCTATATCACAGGGATCACCAGTTACAATGTGCAAAAAGCGATGTCTAGCATTCAGGACAGTGCAAGCT
TTGGAGTCAATTATATTTTAGATGATGTACGTAGAGCCAATTTAAGTGCACCGTCTGCATATATGAATGATCAACAGCCC
AATTCAGGATTAATCCTGACGAGCAGTAATGTAAGTGAATTAAAAGACAATAAACCCTTACTCACCTTAAATGATGCTTA
TCTTACTAAATCTGCTGTAGGTGATGGAAACGTGTCACAGAAAAGTGATCAATTAACTATTCGCTATCAGGCAGTTGAGC
CGGGCTATGATTGTACAGGTAAGACATTGCCTGCGAATACCTATGTAGTTCAACGGTATTTCATAGGAAGTGATAAAGCC
TTGCGATGTGTTTCAGATCAGTACCAACAGACTGGAAATACTATTACGGGAATGGATAATAAAACAAAAACGACATTGGA
TCTTTCGGGAAATGGTCAGATTATTATTCCAAATGTTGATTATATGCGCGTTCAACTTGGATATGTTAAAGGTGCATTGA
ACAATCCAACTGCCATGAGTTATGTCTCAATTGGTGATTATTTGGATACCAATAAAACATCAGCAGTCAAAAAAAATATT
AATGGAATTCAGCAATCTATTAAGCCAAATATTAATGTGATTCAGATTGGATTATTGGTGCAATCCAATGAAACTGCTGG
AAATGACAGCATTGTTCGGAATCGTAATAAACAGGCGTTCGATGTCTTGGGGCAATCTGTCACACTCAATGAAAAAAATC
AGGATACCCGATTGCGCCAAGTAGTTACGCAAACTATTTCCTTAAGAAATGCGATGGGATGGGTTGAAGAGGGGTATGTA
ACGCCATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Type IV Pilus-assembly protein W (PilW)

(153-310)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RBI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]
[3] C Herzberg et al. (2000) comB, a novel competence gene required for natural transformation of Acinetobacter sp. BD413: identification, characterization, and analysis of growth-phase-dependent regulation. Archives of Microbiology 173(3):220-8. [PMID: 10763755]