Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE09_RS06130 Genome accession   NZ_AP027361
Coordinates   1353107..1354150 (+) Length   347 a.a.
NCBI ID   WP_286235319.1    Uniprot ID   -
Organism   Thalassotalea sediminis strain KCTC 42588     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1348107..1359150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE09_RS06110 - 1350379..1350816 (-) 438 WP_286235315.1 DUF4426 domain-containing protein -
  QUE09_RS06115 - 1350890..1351438 (-) 549 WP_286235316.1 YggT family protein -
  QUE09_RS06120 proC 1351534..1352349 (-) 816 WP_286235317.1 pyrroline-5-carboxylate reductase -
  QUE09_RS06125 - 1352365..1353039 (-) 675 WP_286235318.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE09_RS06130 pilT 1353107..1354150 (+) 1044 WP_286235319.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE09_RS06135 pilU 1354160..1355287 (+) 1128 WP_286235320.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE09_RS06140 - 1355357..1355722 (-) 366 WP_286235321.1 hypothetical protein -
  QUE09_RS06145 - 1355753..1356397 (-) 645 WP_286235322.1 oxidoreductase -
  QUE09_RS06150 ruvX 1356400..1356834 (-) 435 WP_286235323.1 Holliday junction resolvase RuvX -
  QUE09_RS06155 - 1356834..1357391 (-) 558 WP_286235324.1 YqgE/AlgH family protein -
  QUE09_RS06160 gshB 1357409..1358362 (-) 954 WP_286235325.1 glutathione synthase -
  QUE09_RS06165 rsmE 1358380..1359126 (-) 747 WP_286235326.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38748.47 Da        Isoelectric Point: 5.8496

>NTDB_id=100790 QUE09_RS06130 WP_286235319.1 1353107..1354150(+) (pilT) [Thalassotalea sediminis strain KCTC 42588]
MDITELLAFSVENNASDLHLSTGVPPLIRVDGDVRKLNIPAFEAKDVNALVYDIMNDRQRKEYEENMEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKVLSLDDLGCPDIFRTISENPRGLVLVTGPTGSGKSTTLAAMIDYINNMKHDHILTIED
PIEFVHENKMCLINQREVHRDTLSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLVKKVGGGRVAAHEIMIGVPAIRNLIREDKVAQMYSAIQTGMQHGMQTMDQCL
QNLVNRGLITRQDAMEKAADKKQFQGF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=100790 QUE09_RS06130 WP_286235319.1 1353107..1354150(+) (pilT) [Thalassotalea sediminis strain KCTC 42588]
ATGGATATTACCGAATTACTCGCATTTAGCGTAGAAAATAATGCTTCGGATTTACATCTTTCTACTGGTGTTCCACCTTT
AATTCGCGTGGATGGCGATGTACGTAAACTTAACATACCTGCGTTTGAAGCCAAAGACGTAAATGCACTTGTTTATGACA
TTATGAATGATCGCCAACGAAAAGAGTACGAAGAAAACATGGAAGTCGACTTTTCCTTTGAAGTGCCAAACTTAGCGCGC
TTCAGGGTAAACGCTTTTAATCAAAATCGCGGTCCAGCTGCTGTATTTCGTACCATTCCTAGCAAAGTACTTTCATTAGA
TGACCTTGGCTGTCCAGATATCTTTAGAACAATTTCTGAAAATCCTCGTGGCTTAGTGCTAGTTACCGGGCCAACAGGTT
CTGGTAAGTCAACGACACTAGCAGCAATGATAGATTACATCAACAATATGAAGCATGATCATATTTTAACGATTGAAGAT
CCTATCGAATTTGTTCACGAAAACAAAATGTGTCTGATCAACCAACGTGAAGTGCATCGCGATACTTTGAGTTTTAACGC
CGCATTACGTTCTGCATTGCGCGAAGATCCTGATGTTATTCTTGTTGGTGAGATGCGTGATTTAGAAACGATTCGTCTTG
CGATGACAGCAGCTGAAACCGGTCATTTAGTGTTTGGTACCTTACATACTACATCTGCGCCTAAAACGATTGACCGTATT
ATTGATGTATTCCCTGCTGAAGAAAAATCTATGGTGCGCTCTATGTTGTCAGAGTCATTACGTGCTGTTATTTCACAAAC
ACTTGTTAAAAAGGTCGGTGGAGGGCGTGTTGCGGCACATGAGATCATGATCGGTGTGCCAGCGATAAGAAACCTTATTC
GTGAAGATAAGGTGGCACAAATGTATTCTGCGATTCAAACGGGGATGCAACATGGTATGCAGACCATGGATCAATGCTTA
CAGAACTTGGTGAATAGAGGTTTAATTACCCGTCAAGATGCGATGGAAAAAGCGGCCGATAAAAAACAATTTCAAGGGTT
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.135

0.772
  pilT Acinetobacter nosocomialis M2

77.616

99.135

0.769
  pilT Acinetobacter baumannii D1279779

77.616

99.135

0.769
  pilT Acinetobacter baumannii strain A118

77.616

99.135

0.769
  pilT Pseudomonas aeruginosa PAK

77.035

99.135

0.764
  pilT Acinetobacter baylyi ADP1

76.744

99.135

0.761
  pilT Legionella pneumophila strain Lp02

74.128

99.135

0.735
  pilT Legionella pneumophila strain ERS1305867

74.128

99.135

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.118

0.692
  pilT Vibrio cholerae strain A1552

71.217

97.118

0.692
  pilT Neisseria meningitidis 8013

68.805

98.847

0.68
  pilT Neisseria gonorrhoeae MS11

68.513

98.847

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

99.424

0.536
  pilU Pseudomonas stutzeri DSM 10701

42.183

97.695

0.412
  pilU Vibrio cholerae strain A1552

41.888

97.695

0.409
  pilU Acinetobacter baylyi ADP1

40

97.983

0.392

Multiple sequence alignment