Detailed information NTG0100791

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QUE09_RS06135
• Genome accession: NZ_AP027361
• Coordinates: 1354160..1355287 (+)
• Length: 375 a.a.
• NCBI ID: WP_286235320.1
• Uniprot ID: -
• Organism: Thalassotalea sediminis strain KCTC 42588
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1349160..1360287

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUE09_RS06110	-	1350379..1350816 (-)	438	WP_286235315.1	DUF4426 domain-containing protein	-
QUE09_RS06115	-	1350890..1351438 (-)	549	WP_286235316.1	YggT family protein	-
QUE09_RS06120	proC	1351534..1352349 (-)	816	WP_286235317.1	pyrroline-5-carboxylate reductase	-
QUE09_RS06125	-	1352365..1353039 (-)	675	WP_286235318.1	YggS family pyridoxal phosphate-dependent enzyme	-
QUE09_RS06130	pilT	1353107..1354150 (+)	1044	WP_286235319.1	type IV pilus twitching motility protein PilT	Machinery gene
QUE09_RS06135	pilU	1354160..1355287 (+)	1128	WP_286235320.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QUE09_RS06140	-	1355357..1355722 (-)	366	WP_286235321.1	hypothetical protein	-
QUE09_RS06145	-	1355753..1356397 (-)	645	WP_286235322.1	oxidoreductase	-
QUE09_RS06150	ruvX	1356400..1356834 (-)	435	WP_286235323.1	Holliday junction resolvase RuvX	-
QUE09_RS06155	-	1356834..1357391 (-)	558	WP_286235324.1	YqgE/AlgH family protein	-
QUE09_RS06160	gshB	1357409..1358362 (-)	954	WP_286235325.1	glutathione synthase	-
QUE09_RS06165	rsmE	1358380..1359126 (-)	747	WP_286235326.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 41260.15 Da
• Isoelectric Point: 5.5648

>NTDB_id=100791 QUE09_RS06135 WP_286235320.1 1354160..1355287(+) (pilU) [Thalassotalea sediminis strain KCTC 42588]
MLSFHNYLEKMVQHDASDMFVTAKLPVSAKVNGELTPIDEAVLTPEQALELVHNAMNDKQKKQFDQEKECNFAISIDGIG
RFRVSAFWQRDMAGMVIRRIVTDIPDADDLGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNKNSRGHILTIE
DPVEFVHEHGKSMVTQREVGLDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALAFAETGHLCIATLHANNANQAIDR
IMHLVPAEQHGKLLFDLALNLRGIVAQQLIPTRDGNGRVAAIEILLNSPYIAELIKKGEVGDIKEVMEKSTEQGMQTFDQ
ALFNLYQRGLINYADAIHHADSPNDLRLMIKLRSNDQGGSGSLSGVTIDGLDPKD

Nucleotide

• Length: 1128 bp

>NTDB_id=100791 QUE09_RS06135 WP_286235320.1 1354160..1355287(+) (pilU) [Thalassotalea sediminis strain KCTC 42588]
ATGCTAAGTTTTCATAATTACCTCGAAAAAATGGTGCAGCATGATGCTTCTGATATGTTTGTTACCGCTAAACTGCCTGT
CAGTGCGAAGGTCAACGGCGAACTTACGCCTATTGATGAAGCCGTATTAACGCCAGAACAAGCTTTAGAGCTTGTACATA
ATGCAATGAATGACAAGCAGAAAAAGCAATTTGACCAAGAAAAAGAATGTAACTTTGCTATTTCAATTGATGGTATTGGC
CGTTTTCGTGTTTCCGCTTTTTGGCAGCGCGACATGGCTGGTATGGTTATTCGCCGTATTGTTACTGATATACCAGATGC
AGACGATTTAGGACTCCCTTCGGTACTTAAAGACGTTGTTATGTCAAAGCGTGGTTTAGTATTATTCGTTGGTGGTACAG
GTACGGGTAAGTCGACGTCAATGGCGGCTTTAATTGGTTATCGCAATAAAAACTCGAGAGGCCATATCCTTACGATTGAA
GATCCTGTGGAATTTGTCCATGAACATGGAAAATCAATGGTAACGCAACGCGAGGTTGGCCTAGATACGGAAAGTTTTGA
TGCCGCGTTGAAGAGTTCGTTGCGTCAAGCGCCAGATGTTATTCTTATTGGTGAGATCAGAAGCCAAGAAATTATGGAGC
ATGCGTTGGCGTTTGCTGAAACAGGGCACTTATGTATTGCTACATTACATGCCAATAATGCTAACCAAGCGATTGATCGT
ATCATGCACTTAGTACCCGCAGAACAACATGGAAAATTGTTATTTGATTTAGCATTGAACCTAAGAGGTATTGTTGCACA
GCAATTGATACCAACGCGAGATGGTAATGGTCGTGTTGCCGCTATCGAAATTTTACTTAATTCACCTTACATCGCTGAAC
TTATTAAGAAAGGCGAAGTAGGCGATATTAAAGAAGTAATGGAAAAATCTACCGAGCAAGGCATGCAAACCTTTGACCAA
GCACTGTTTAATTTATATCAGCGAGGTTTAATTAATTATGCGGATGCGATACACCATGCTGATTCACCAAATGATCTACG
CTTAATGATTAAACTACGCAGTAATGATCAAGGTGGTAGTGGTTCATTGTCGGGCGTAACAATAGACGGTTTAGATCCAA
AAGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (106-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.905	95.2	0.589
pilU	Vibrio cholerae strain A1552	57.065	98.133	0.56
pilU	Acinetobacter baylyi ADP1	57.386	93.867	0.539
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.739	92	0.384
pilT	Pseudomonas aeruginosa PAK	41.617	89.067	0.371