Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABL848_RS14635 Genome accession   NZ_CP157611
Coordinates   3124286..3125896 (+) Length   536 a.a.
NCBI ID   WP_093203954.1    Uniprot ID   A0A1H7T4F4
Organism   Variovorax sp. NFACC28     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3119286..3130896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL848_RS14610 (ABL848_14590) argS 3119350..3121035 (+) 1686 WP_093301631.1 arginine--tRNA ligase -
  ABL848_RS14615 (ABL848_14595) - 3121047..3121817 (+) 771 WP_093301777.1 SPOR domain-containing protein -
  ABL848_RS14620 (ABL848_14600) - 3121974..3122621 (+) 648 WP_093203947.1 thiol:disulfide interchange protein DsbA/DsbL -
  ABL848_RS14625 (ABL848_14605) lptA 3122754..3123473 (+) 720 WP_093301632.1 lipopolysaccharide transport periplasmic protein LptA -
  ABL848_RS14630 (ABL848_14610) lptB 3123470..3124264 (+) 795 WP_093301634.1 LPS export ABC transporter ATP-binding protein -
  ABL848_RS14635 (ABL848_14615) rpoN/rpoN1 3124286..3125896 (+) 1611 WP_093203954.1 RNA polymerase factor sigma-54 Machinery gene
  ABL848_RS14640 (ABL848_14620) - 3125910..3127511 (+) 1602 WP_093301635.1 phospholipase D family protein -
  ABL848_RS14645 (ABL848_14625) - 3127525..3128175 (-) 651 WP_093301637.1 LysE family translocator -
  ABL848_RS14650 (ABL848_14630) - 3128194..3128832 (-) 639 WP_093301638.1 YceI family protein -
  ABL848_RS14655 (ABL848_14635) - 3128901..3129494 (-) 594 WP_418116347.1 YceI family protein -
  ABL848_RS14660 (ABL848_14640) - 3129497..3130090 (-) 594 WP_093301640.1 cytochrome b -

Sequence

Protein


Download         Length: 536 a.a.        Molecular weight: 58832.82 Da        Isoelectric Point: 4.5291

>NTDB_id=1007677 ABL848_RS14635 WP_093203954.1 3124286..3125896(+) (rpoN/rpoN1) [Variovorax sp. NFACC28]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDTADAPVPRDEGGDGGEGG
DGEGDFASPAASSASTASTETVTSTADTDGPAADISEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELA
RSQESLQSFLHRQALSLRLDENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPAG
VGARDLGECLSIQLRAMASEDETEEEEQVRKTAIAICKQPMELLARRDFKRLATLTRSNEDLVRSALQIIARLEPKPGRS
FVDVERNIIIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTI
LRVSNAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAV
RALIKQFVGSESVKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1611 bp        

>NTDB_id=1007677 ABL848_RS14635 WP_093203954.1 3124286..3125896(+) (rpoN/rpoN1) [Variovorax sp. NFACC28]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCATCTGGCGCTGACGCCACAGCTGCAGCAGTCGATCAGGCTGCTTCA
ACTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAGCGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTCGACACGGCCGATGCCCCGGTTCCGCGCGATGAAGGCGGTGACGGTGGTGAAGGCGGG
GACGGCGAGGGCGACTTCGCTTCGCCGGCCGCCAGCAGCGCATCCACTGCTTCCACCGAAACCGTCACCAGCACGGCCGA
CACCGACGGCCCCGCCGCCGACATCTCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACG
ACAGCGAATGGGGCAGCGACGCGCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCG
CGCAGCCAGGAGTCGCTGCAGTCCTTCCTGCATCGCCAGGCGCTGAGCCTGCGCCTCGACGAGAACGACAGCGCCGCGCT
GCGCTTCCTGATCGAGTCGCTCAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCCCTGGCCTCGGGCCTGGCCGGCG
ACGACAACGACCAGTTCGACGACCTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTCCAGAGCCTGGAGCCCGCCGGC
GTCGGCGCGCGCGACCTCGGCGAATGCCTGAGCATCCAGCTGCGCGCCATGGCCAGCGAGGACGAGACCGAGGAAGAGGA
GCAGGTCCGCAAGACCGCCATCGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGCCGCGACTTCAAGCGCCTGGCCA
CGCTCACCCGCAGCAACGAGGACCTGGTGCGCTCGGCGCTGCAGATCATCGCGCGGCTGGAGCCCAAGCCGGGCCGCAGC
TTCGTCGACGTCGAGCGCAACATCATCATTCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACGAACGTGAAGTTCCG
CGTCATGCTCAACCCCGAGGTGATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCG
AGGGCAGCCAGGCGCTGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATC
CTGCGCGTGAGCAACGCCATCGTCGAGCGGCAGAAGAGCTACTTCGTGCACGGCGAGCTCGCGATGCGCCCGCTGGTGCT
GCGCGAGATCGCCGACGAGCTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCT
TCGGCACGGTCGAGCTCAAGTACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTG
CGCGCGCTCATCAAGCAGTTCGTCGGCTCCGAGAGCGTGAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAA
GGAACAGGGCATCGAGTGCGCGCGCCGCACCGTCGCCAAGTACCGCGAAGCGCTTCGCATCGCGCCCGCCAACCTGCGCA
AAGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(378-534)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(157-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H7T4F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.397

100

0.515

Multiple sequence alignment