Detailed information NTG1007040

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABK819_RS02020
• Genome accession: NZ_CP157460
• Coordinates: 424324..425472 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 419324..430472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABK819_RS01995 (ABK819_01995)	-	420475..420771 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABK819_RS02000 (ABK819_02000)	-	420768..421361 (-)	594	WP_003084544.1	YggT family protein	-
ABK819_RS02005 (ABK819_02005)	proC	421372..422193 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ABK819_RS02010 (ABK819_02010)	-	422205..422897 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABK819_RS02015 (ABK819_02015)	pilT	423112..424146 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABK819_RS02020 (ABK819_02020)	pilU	424324..425472 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ABK819_RS02025 (ABK819_02025)	czcD	425480..426379 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABK819_RS02030 (ABK819_02030)	-	426533..426937 (+)	405	WP_003084560.1	NINE protein	-
ABK819_RS02035 (ABK819_02035)	-	427180..428553 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ABK819_RS02040 (ABK819_02040)	-	428550..429734 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1007040 ABK819_RS02020 WP_003110016.1 424324..425472(+) (pilU) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1007040 ABK819_RS02020 WP_003110016.1 424324..425472(+) (pilU) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382