Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ABKP85_RS03435 Genome accession   NZ_CP157379
Coordinates   651802..653607 (+) Length   601 a.a.
NCBI ID   WP_014567973.1    Uniprot ID   K7DWB4
Organism   Lactococcus cremoris strain NZ3900     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 644106..654922 651802..653607 within 0
IScluster/Tn 644518..654248 651802..653607 within 0


Gene organization within MGE regions


Location: 644106..654922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKP85_RS03390 (ABKP85_03390) - 644106..644366 (+) 261 WP_014734952.1 hypothetical protein -
  ABKP85_RS03400 (ABKP85_03400) - 645660..646484 (+) 825 WP_011834656.1 polysaccharide deacetylase family protein -
  ABKP85_RS03405 (ABKP85_03405) brnQ 646571..647920 (-) 1350 WP_011834657.1 branched-chain amino acid transport system II carrier protein -
  ABKP85_RS03410 (ABKP85_03410) - 648136..648408 (-) 273 WP_011834658.1 GNAT family N-acetyltransferase -
  ABKP85_RS03415 (ABKP85_03415) - 648412..648501 (-) 90 WP_080941974.1 (4Fe-4S)-binding protein -
  ABKP85_RS03425 (ABKP85_03425) - 650307..650861 (-) 555 WP_011834703.1 recombinase family protein -
  ABKP85_RS03430 (ABKP85_03430) coiA 651092..651739 (+) 648 WP_231097785.1 competence protein CoiA family protein Machinery gene
  ABKP85_RS03435 (ABKP85_03435) pepF 651802..653607 (+) 1806 WP_014567973.1 oligoendopeptidase F Regulator
  ABKP85_RS03440 (ABKP85_03440) - 653610..653729 (+) 120 Protein_649 O-methyltransferase -
  ABKP85_RS03445 (ABKP85_03445) - 653716..654248 (-) 533 Protein_650 IS3 family transposase -
  ABKP85_RS03450 (ABKP85_03450) - 654341..654922 (+) 582 WP_011669105.1 recombinase family protein -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69862.75 Da        Isoelectric Point: 5.0530

>NTDB_id=1006450 ABKP85_RS03435 WP_014567973.1 651802..653607(+) (pepF) [Lactococcus cremoris strain NZ3900]
MAKNRNEIPEKLTWDLTTIYKTDKEWEAELTRIKSELSLVEETDPGHLLDSAESLLTITEKMLSISQQVEKLYVYASMKN
DQDTREAKYQEYQSKATALYVKFGEVYAFYEPEFLKISKEVYNKWLGELQKLKNYDHMFERLFAKKAHILSQKEEKLLAA
AGEIFESPSETFEIFDNADIKLPMVKNESDEMIQLTHGNYSSLMESKNRGVRKAAYKALYSNYEQYQHTYAKTLQTNVKV
HNLNAQIRSYDSARQAALANNFVPEKVYDVLMEAIHQHLPLLHRYIELRKKILGITDLKMYDIYTPLSNLDYKFNYEDGV
KKAEEVLAIFGKEYKGKVKAAFEQRWIDVEENIGKRSGAYSGGSYDTNAFMLLNWQETLDDLFTLVHETGHSMHSAFTRE
NQPYVYGNYPIFLAEIASTTNENILTETLLKESKDDKERFALLNHWLDSFRGTVFRQSQFAEFEQKIHEADAAGEVLTSE
YLNSLYGEINEKYYNLAVKENPEIQYEWARIPHFYYNFYVFQYATGFAAATFLAEKVVHGSTEDRQKYLEYLKAGSSAYP
LEVIAKAGVDMESTDYLDAAFELFENRLSELEKLVEKGVHL

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=1006450 ABKP85_RS03435 WP_014567973.1 651802..653607(+) (pepF) [Lactococcus cremoris strain NZ3900]
ATGGCTAAGAATAGAAATGAAATTCCTGAAAAATTAACTTGGGATTTGACAACAATTTATAAAACAGATAAAGAATGGGA
AGCAGAGCTAACTAGAATAAAAAGCGAACTTTCACTCGTTGAAGAGACAGACCCTGGTCATTTACTTGATTCAGCTGAAA
GTCTTCTGACAATTACCGAAAAAATGCTATCCATTTCTCAACAAGTTGAAAAACTATATGTTTATGCTTCAATGAAAAAT
GATCAAGATACCAGAGAGGCTAAATATCAGGAGTATCAATCAAAAGCAACAGCCCTCTATGTGAAATTCGGAGAGGTCTA
TGCCTTCTATGAACCTGAATTTTTGAAAATTTCAAAAGAAGTTTATAACAAATGGTTGGGGGAGCTTCAAAAATTAAAAA
ACTATGACCATATGTTTGAGCGCCTTTTTGCAAAAAAAGCGCATATTCTTAGTCAAAAAGAGGAAAAGTTATTGGCTGCG
GCTGGTGAAATTTTTGAAAGTCCTTCAGAAACTTTTGAAATTTTTGATAATGCGGATATTAAGTTACCTATGGTCAAAAA
TGAATCTGATGAGATGATCCAACTCACGCATGGAAATTACTCTTCCTTAATGGAAAGTAAGAATAGGGGAGTACGAAAAG
CAGCCTACAAAGCACTTTATAGTAATTATGAACAATATCAGCATACTTATGCAAAGACCTTACAGACTAATGTAAAAGTC
CATAATCTTAATGCCCAAATCCGCTCTTATGATTCAGCCCGTCAAGCTGCTCTGGCTAATAATTTTGTTCCAGAAAAAGT
TTACGATGTTTTGATGGAGGCCATTCATCAACATTTGCCACTTTTACATCGTTATATTGAACTACGCAAGAAAATTTTAG
GAATTACTGATTTAAAGATGTATGATATCTATACTCCATTATCTAATTTAGATTATAAATTTAACTATGAAGATGGAGTG
AAAAAAGCAGAAGAAGTTTTAGCGATATTTGGTAAAGAATATAAAGGAAAAGTTAAAGCAGCTTTTGAACAAAGATGGAT
TGATGTCGAAGAAAATATCGGAAAACGTTCGGGTGCTTATTCAGGTGGATCTTATGATACCAATGCTTTTATGCTTCTAA
ATTGGCAAGAAACGTTAGATGATCTTTTTACTTTAGTTCATGAAACGGGGCACTCAATGCATAGTGCTTTCACGCGTGAA
AACCAACCATATGTTTATGGGAATTATCCAATCTTTTTAGCTGAAATTGCCTCAACTACAAATGAAAATATTTTAACTGA
AACTTTATTAAAGGAAAGTAAAGATGATAAGGAGCGCTTCGCCCTTTTGAACCACTGGCTTGATAGTTTCCGTGGAACTG
TTTTCCGTCAGAGTCAATTTGCCGAATTTGAACAAAAAATTCATGAAGCAGATGCTGCCGGTGAAGTTTTAACAAGTGAA
TACTTAAACTCACTTTATGGCGAAATAAATGAAAAATACTATAATTTAGCAGTCAAAGAAAATCCAGAAATTCAGTATGA
ATGGGCTAGAATTCCGCATTTTTATTATAATTTCTATGTTTTTCAATACGCAACAGGTTTTGCTGCGGCAACTTTCTTGG
CTGAAAAAGTTGTTCATGGTTCAACTGAGGATCGTCAAAAATATCTTGAATATTTAAAGGCTGGTTCTTCAGCTTATCCT
TTAGAAGTAATTGCCAAAGCTGGAGTTGATATGGAATCTACCGATTATTTAGATGCTGCCTTTGAGCTTTTTGAAAATCG
ATTGAGTGAATTAGAAAAATTAGTTGAAAAAGGAGTCCATCTTTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-582)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7DWB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

64.06

100

0.641

Multiple sequence alignment