Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ABI226_RS20245 Genome accession   NZ_CP157216
Coordinates   3850237..3851382 (+) Length   381 a.a.
NCBI ID   WP_003227549.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis isolate FELIX_MS22     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3845237..3856382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI226_RS20220 (ABI226_20220) albF 3845253..3846533 (+) 1281 WP_003242974.1 pitrilysin family protein -
  ABI226_RS20225 (ABI226_20225) albG 3846530..3847231 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  ABI226_RS20230 (ABI226_20230) ywhL 3847237..3848613 (-) 1377 WP_003244415.1 YncE family protein -
  ABI226_RS20235 (ABI226_20235) ywhK 3848652..3850007 (-) 1356 WP_003227552.1 YncE family protein -
  ABI226_RS20240 (ABI226_20240) - 3850004..3850234 (-) 231 WP_009968328.1 hypothetical protein -
  ABI226_RS20245 (ABI226_20245) rapF 3850237..3851382 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  ABI226_RS20250 (ABI226_20250) phrF 3851366..3851485 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  ABI226_RS20255 (ABI226_20255) ywhH 3851584..3852057 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  ABI226_RS20260 (ABI226_20260) speB 3852089..3852961 (-) 873 WP_003227545.1 agmatinase -
  ABI226_RS20265 (ABI226_20265) speE 3853022..3853852 (-) 831 WP_003227543.1 spermidine synthase -
  ABI226_RS20270 (ABI226_20270) pbpG 3854054..3856129 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45609.09 Da        Isoelectric Point: 5.1503

>NTDB_id=1004876 ABI226_RS20245 WP_003227549.1 3850237..3851382(+) (rapF) [Bacillus subtilis subsp. subtilis isolate FELIX_MS22]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1004876 ABI226_RS20245 WP_003227549.1 3850237..3851382(+) (rapF) [Bacillus subtilis subsp. subtilis isolate FELIX_MS22]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_12)

(223-290)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572

Multiple sequence alignment