Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ABI424_RS02375 Genome accession   NZ_CP157211
Coordinates   551886..552425 (-) Length   179 a.a.
NCBI ID   WP_012341221.1    Uniprot ID   -
Organism   Histophilus somni strain 23-038383_S91     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546886..557425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI424_RS02365 (ABI424_000474) dnaJ 548128..549249 (+) 1122 WP_012341223.1 molecular chaperone DnaJ -
  ABI424_RS02370 (ABI424_000475) nrdA 549533..551803 (+) 2271 WP_012341222.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  ABI424_RS02375 (ABI424_000476) pilF/pilF2 551886..552425 (-) 540 WP_012341221.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABI424_RS02380 (ABI424_000477) - 552456..553574 (-) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ABI424_RS02385 (ABI424_000478) dnaX 553827..555830 (-) 2004 WP_012341219.1 DNA polymerase III subunit gamma/tau -
  ABI424_RS02390 (ABI424_000479) apt 555861..556400 (-) 540 WP_012341218.1 adenine phosphoribosyltransferase -
  ABI424_RS02395 (ABI424_000480) lpxM 556471..557424 (-) 954 WP_012341217.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20670.59 Da        Isoelectric Point: 9.3139

>NTDB_id=1004667 ABI424_RS02375 WP_012341221.1 551886..552425(-) (pilF/pilF2) [Histophilus somni strain 23-038383_S91]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1004667 ABI424_RS02375 WP_012341221.1 551886..552425(-) (pilF/pilF2) [Histophilus somni strain 23-038383_S91]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(109-130)

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436

Multiple sequence alignment