Detailed information NTG1004626

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: ABI122_RS05990
• Genome accession: NZ_CP157205
• Coordinates: 1152837..1156562 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000572298.1
• Uniprot ID: A0A9W5Q937
• Organism: Bacillus thuringiensis strain 9.1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1147837..1161562

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABI122_RS05975 (ABI122_05975)	-	1147995..1148588 (+)	594	WP_074554821.1	TVP38/TMEM64 family protein	-
ABI122_RS05980 (ABI122_05980)	lepB	1148645..1149208 (+)	564	WP_000751897.1	signal peptidase I	-
ABI122_RS05985 (ABI122_05985)	addB	1149325..1152840 (+)	3516	WP_130055523.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
ABI122_RS05990 (ABI122_05990)	addA	1152837..1156562 (+)	3726	WP_000572298.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
ABI122_RS05995 (ABI122_05995)	-	1156575..1156862 (+)	288	WP_130055522.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
ABI122_RS06000 (ABI122_06000)	gerPF	1157000..1157215 (-)	216	WP_001141566.1	spore germination protein GerPF	-
ABI122_RS06005 (ABI122_06005)	-	1157258..1157644 (-)	387	WP_000902324.1	spore germination protein GerPE	-
ABI122_RS06010 (ABI122_06010)	gerPD	1157660..1157854 (-)	195	WP_001052807.1	spore germination protein GerPD	-
ABI122_RS06015 (ABI122_06015)	gerPC	1157861..1158475 (-)	615	WP_001070760.1	spore germination protein GerPC	-
ABI122_RS06020 (ABI122_06020)	gerPB	1158543..1158749 (-)	207	WP_001012508.1	spore germination protein GerPB	-
ABI122_RS06025 (ABI122_06025)	gerPA	1158764..1158985 (-)	222	WP_001111187.1	spore germination protein GerPA	-
ABI122_RS06030 (ABI122_06030)	-	1159082..1159261 (-)	180	WP_000462841.1	aspartyl-phosphate phosphatase Spo0E family protein	-
ABI122_RS06035 (ABI122_06035)	-	1159509..1160408 (+)	900	WP_001213071.1	fumarylacetoacetate hydrolase family protein	-
ABI122_RS06040 (ABI122_06040)	-	1160444..1160725 (-)	282	WP_000926857.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142717.88 Da
• Isoelectric Point: 4.8010

>NTDB_id=1004626 ABI122_RS05990 WP_000572298.1 1152837..1156562(+) (addA) [Bacillus thuringiensis strain 9.1]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=1004626 ABI122_RS05990 WP_000572298.1 1152837..1156562(+) (addA) [Bacillus thuringiensis strain 9.1]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGGCG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGTTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTTTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATACTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTGCAACGTATGATTTTAGC
GCTTCATACAGAATCAAGGGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCATTGAAACTCTGCAAGCAGATGTAGCATTACT
TGGAACGCTATCATCAGCTGCTCGTGAATCGTGGACAAGTGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTAGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGCTGACGCCGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGTCCGGTGCAATA
CCGCGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATACGCTGACCTCGCGACTGGTTATTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCCGCTGTACTTCGTTCCCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGATATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACACTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTTAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535