Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ABI122_RS05985 Genome accession   NZ_CP157205
Coordinates   1149325..1152840 (+) Length   1171 a.a.
NCBI ID   WP_130055523.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain 9.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1144325..1157840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI122_RS05940 (ABI122_05940) - 1144627..1144917 (-) 291 Protein_1072 DUF6584 family protein -
  ABI122_RS05945 (ABI122_05945) - 1145067..1145273 (-) 207 Protein_1073 DUF6584 family protein -
  ABI122_RS05950 (ABI122_05950) cspA 1145556..1145759 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  ABI122_RS05955 (ABI122_05955) - 1145886..1146011 (+) 126 WP_282025502.1 hypothetical protein -
  ABI122_RS05960 (ABI122_05960) - 1146008..1146160 (-) 153 WP_071740672.1 hypothetical protein -
  ABI122_RS05965 (ABI122_05965) - 1146508..1146693 (-) 186 WP_000391373.1 hypothetical protein -
  ABI122_RS05970 (ABI122_05970) - 1146978..1147559 (+) 582 WP_000444443.1 competence protein ComK -
  ABI122_RS05975 (ABI122_05975) - 1147995..1148588 (+) 594 WP_074554821.1 TVP38/TMEM64 family protein -
  ABI122_RS05980 (ABI122_05980) lepB 1148645..1149208 (+) 564 WP_000751897.1 signal peptidase I -
  ABI122_RS05985 (ABI122_05985) addB 1149325..1152840 (+) 3516 WP_130055523.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ABI122_RS05990 (ABI122_05990) addA 1152837..1156562 (+) 3726 WP_000572298.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ABI122_RS05995 (ABI122_05995) - 1156575..1156862 (+) 288 WP_130055522.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ABI122_RS06000 (ABI122_06000) gerPF 1157000..1157215 (-) 216 WP_001141566.1 spore germination protein GerPF -
  ABI122_RS06005 (ABI122_06005) - 1157258..1157644 (-) 387 WP_000902324.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134162.52 Da        Isoelectric Point: 5.2131

>NTDB_id=1004625 ABI122_RS05985 WP_130055523.1 1149325..1152840(+) (addB) [Bacillus thuringiensis strain 9.1]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTAEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVRATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAALPAGVLYFHIHNPIVEMKGDATEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=1004625 ABI122_RS05985 WP_130055523.1 1149325..1152840(+) (addB) [Bacillus thuringiensis strain 9.1]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGTGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATACGATGATTTTGAACGTGCTTTAATTGGAAAATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGACGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGACGAAAAATATCGTTACCGGGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACATCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTGCTGAAGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACGGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTGTTTTTAGAAGAACTGGATGTTCCGAAAAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACATTTGTAGAGATGCTTGGGGAAGAGAAA
ATGTCTCTTTCTATGTTTACTGACGTTATGTCGACTGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGATTATCTGATGTGAGAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAATGATTTATCACGTTCAGAACAAATTTCATATGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGCACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATTGCGCCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGATCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCGCAGACATGGATGAAAAAAGGCCATGCAGCATTACCTGCTGGTG
TATTGTATTTCCATATTCATAATCCAATCGTTGAGATGAAGGGTGACGCAACGGAAGCAGAAATTGAAAAAGAAATTTTG
AAGAAATTTAAAATGAAAGGACTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACCGGAAG
CTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTTCAAAAATACGTACACCATACGTTTGAAAACATCGGGAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAGTCTGTTTGTCAATTTGATGAGTCACTGGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.403

100

0.494

Multiple sequence alignment