Detailed information NTG0100303

Bioinformatically predicted

Overview

• Name: radC
• Type: Machinery gene
• Locus tag: ECRM13516_RS22255
• Genome accession: NZ_CP006262
• Coordinates: 4385447..4386115 (-)
• Length: 222 a.a.
• NCBI ID: WP_001297375.1
• Uniprot ID: P65957
• Organism: Escherichia coli O145:H28 str. RM13516
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4380447..4391115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECRM13516_RS22225 (ECRM13516_4419)	rfaQ	4380625..4381683 (-)	1059	WP_000360416.1	lipopolysaccharide core heptosyltransferase RfaQ	-
ECRM13516_RS22230 (ECRM13516_4420)	waaA	4382096..4383373 (+)	1278	WP_000891562.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
ECRM13516_RS22235 (ECRM13516_4421)	coaD	4383381..4383860 (+)	480	WP_001171873.1	pantetheine-phosphate adenylyltransferase	-
ECRM13516_RS22240 (ECRM13516_4422)	mutM	4383899..4384708 (-)	810	WP_001114533.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
ECRM13516_RS22245 (ECRM13516_4423)	rpmG	4384806..4384973 (-)	168	WP_001051798.1	50S ribosomal protein L33	-
ECRM13516_RS22250 (ECRM13516_4424)	rpmB	4384994..4385230 (-)	237	WP_000091955.1	50S ribosomal protein L28	-
ECRM13516_RS22255 (ECRM13516_4425)	radC	4385447..4386115 (-)	669	WP_001297375.1	DNA repair protein RadC	Machinery gene
ECRM13516_RS22260 (ECRM13516_4427)	coaBC	4386287..4387507 (+)	1221	WP_000050121.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ECRM13516_RS22265 (ECRM13516_4428)	dut	4387485..4387943 (+)	459	WP_000976070.1	dUTP diphosphatase	-
ECRM13516_RS22270 (ECRM13516_4429)	slmA	4388050..4388646 (+)	597	WP_000818601.1	nucleoid occlusion factor SlmA	-
ECRM13516_RS22275 (ECRM13516_4430)	pyrE	4388683..4389324 (-)	642	WP_000806177.1	orotate phosphoribosyltransferase	-
ECRM13516_RS22280 (ECRM13516_4431)	rph	4389390..4390106 (-)	717	WP_001247093.1	ribonuclease PH	-
ECRM13516_RS22285 (ECRM13516_4432)	yicC	4390233..4391096 (+)	864	WP_000621340.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 222 a.a.
• Molecular weight: 25258.35 Da
• Isoelectric Point: 7.9748

>NTDB_id=100303 ECRM13516_RS22255 WP_001297375.1 4385447..4386115(-) (radC) [Escherichia coli O145:H28 str. RM13516]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide

• Length: 669 bp

>NTDB_id=100303 ECRM13516_RS22255 WP_001297375.1 4385447..4386115(-) (radC) [Escherichia coli O145:H28 str. RM13516]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

• RadC-like JAB domain (RadC) (103-220)

• UPF0758 N-terminal (UPF0758_N) (9-77)

3D structure

• AlphaFold DB: P65957

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radC	Haemophilus influenzae Rd KW20	49.55	100	0.496