Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100173
Name   oriT_pEC3 experimental
Organism   Erwinia carotovora
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   D45188 (797..804 [-], 8 nt)
oriT length   8 nt
IRs (inverted repeats)     _
Location of nic site      4..5
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   _

  oriT sequence  


Download         Length: 8 nt

>oriT_pEC3
CTGGCTTA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Nomura N et al. (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]


Relaxosome

This oriT is a component of a relaxosome.

Relaxosome name   RelaxosomepEC3 in_silico
oriT   oriT_pEC3 experimental
Relaxase   MobA_pEC3 experimental (MOBP)
Auxiliary protein   MobB_pEC3 experimental, MobC_pEC3 experimental

  Reference


[1] Nomura N et al. (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]


Relaxase

ID   166 GenBank   BAA08130
Name   MobA_pEC3 experimental UniProt ID   Q47385
Length   498 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 498 a.a.        Molecular weight: 55532.66 Da        Isoelectric Point: 7.2817

>BAA08130.1 mobA (plasmid) [Pectobacterium carotovorum]
MIVKFHPRGRGGGAGPVDYLLGKDRNRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERILMPGLDKDQYSVLWVEHQDKGRLELNFLVPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPEAKQEAAQTITRGLLALASSGELKTRQDVTEAVESAGFEVLRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESARAEYRRDAESRIQRAREVCQSGTERKREENQRR
YPRPRPEAVFSHEPAHERDAAHGQPDVADHRAGFRPADRVERGHSVVAGAADTRQLHDHPGAEEHAGDAV
REEQRRATHDLWRAETPLREGEPGSGNVRRGFELDDTGGEIAHDGAGKTVVERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAKPVVERLNVIEQRRQYEHSLQLQKERGSAHQRH
YSGRSMEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure


Source ID Structure
AlphaFold DB Q47385

  Reference


[1] Nomura N et al. (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]


Auxiliary protein

ID   238 GenBank   BAA08131
Name   MobB_pEC3 experimental UniProt ID   Q47386
Length   161 a.a. PDB ID   _
Note   MbeB-like, N-term conserved region; pfam04837

  Auxiliary protein sequence


Download         Length: 161 a.a.        Molecular weight: 18027.63 Da        Isoelectric Point: 9.1972

>BAA08131.1 mobB (plasmid) [Pectobacterium carotovorum]
MNSLLTLAKDLEQKSKAHEQSTGEMLKAAFSEHEKSVRAELSESEKRISAAILDHDRKLSSVMSQRTKGM
LRMVSQTWLTIVLVSVLLIASSAGILWWQGQQILDNYTTIREQKSTQAMLSERNSGVQLTTCGEQRRRCV
RVNPEAGTFGEDSSWMILAGK

  Protein domains


Predicted by InterproScan.

MbeB-like, N-term conserved region (MbeB_N)

(1-52)


  Protein structure


Source ID Structure
AlphaFold DB Q47386

  Reference


[1] Nomura N et al. (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]

ID   239 GenBank   BAA08129
Name   MobC_pEC3 experimental UniProt ID   Q47384
Length   114 a.a. PDB ID   _
Note   Bacterial mobilisation protein (MobC); pfam05713

  Auxiliary protein sequence


Download         Length: 114 a.a.        Molecular weight: 12373.26 Da        Isoelectric Point: 10.0335

>BAA08129.1 mobC (plasmid) [Pectobacterium carotovorum]
MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAGMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQI
ARQVNAGGGSGHDRVQVVAALMAIDAGLKRLRHAVLEKGADDDS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(57-101)


  Protein structure


Source ID Structure
AlphaFold DB Q47384

  Reference


[1] Nomura N et al. (1996) Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora. Gene. 170(1):57-62. [PMID:8621089]


Host bacterium

ID   166 GenBank   D45188
Plasmid name   pEC3 Incompatibility group   ColRNAI
Plasmid size   3000 bp Coordinate of oriT [Strand]   797..804 [-]
Host baterium   Erwinia carotovora

Cargo genes

Drug resistance gene   _
Virulence gene   _
Metal resistance gene   _
Degradation gene   _
Symbiosis gene   -
Anti-CRISPR   -