oriTDB
The information of the oriT region
oriTDB accession number 100176
Name oriT_R751  experimental
Sequence Completeness intact
oriT length 99 nt
oriT sequence GAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTG CCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACA
IR (inverted repeat)[13-31] [34-52] nt  (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site [60-61] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note _
L:13-31;R:34-52;D:60-61
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Waters VL et al (1991) Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids. Proc Natl Acad Sci U S A. 88(4):1456-60. [PMID:1996345]
[2] Furuya N et al (1991) Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids. J Bacteriol. 173(20):6612-7. [PMID:1917882]
[3] Pansegrau W et al (1988) The origin of conjugative IncP plasmid transfer: interaction with plasmid-encoded products and the nucleotide sequence at the relaxation site. Biochim Biophys Acta. 951(2-3):365-74. [PMID:2850014]
[4] Fürste JP et al (1989) Conjugative transfer of promiscuous IncP plasmids: interaction of plasmid-encoded products with the transfer origin. Proc Natl Acad Sci U S A. 86(6):1771-5. [PMID:2538813]
I. Information of Relaxase
ID 169
Name TraI_R751 insolico
GenBank accession number NP_044272
Family MOBP
Length 747 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note to recognize the conserved nick region sequence
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA
INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL
SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA
LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSEIQAINKQFQQ
ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA
VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF
KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA
ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL
SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI
AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR
LTRIAVGRDAVSITAKGSIKTSKGRSR
Reference
[1] Waters VL et al (1991) Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids. Proc Natl Acad Sci U S A. 88(4):1456-60. [PMID:1996345]
[2] Pansegrau W et al (1988) The origin of conjugative IncP plasmid transfer: interaction with plasmid-encoded products and the nucleotide sequence at the relaxation site. Biochim Biophys Acta. 951(2-3):365-74. [PMID:2850014]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 242 TraH_R751 insolico NP_044273 130 aa relaxosome stabilisation
2 243 TraJ_R751 insolico NP_044275 124 aa _
3 244 TraK_R751 insolico NP_044276 132 aa _
Information of T4CP
ID 169
Name TraG_R751 insolico
SecReT4 accesion number 10955242
GenBank accession number CAA38327
Family VirD4/TraG
Length 637
UniProt ID Q00184
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624
TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569
TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586
Note Type IV Secretion System Coupling Protein
Protein sequence [Download] MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV
YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA
RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT
YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG
VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD
DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV
LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ
DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT
AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL
YFKDPVFSARAAIPAPKVSDRLRAVAQADTEGEGITI

Reference
[1] Waters VL et al (1991) Conjugative transfer of promiscuous IncP plasmids: interaction of plasmid-encoded products with the transfer origin. Proc Natl Acad Sci U S A. 88(4):1456-60. [PMID:1996345]
Information of plasmid
ID 169
Plasmid name R751
GenBank accession number NC_001735.4
Incompatibility group IncP1
Genome size 53423 bp
Coordinate of oriT  [Strand] 51526..51624 [+]
Drug resistance insolico resistance to trimethoprim, quaternary ammonium compounds (qacE)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterobacter aerogenes [548]
Reference
[1] Thorsted PB et al (1998) Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone. J Mol Biol. 282(5):969-90. [PMID:9753548]

Microbial Bioinformatics Group in MML, SJTU