Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/AbrB-PIN |
| Location | 1782460..1783108 | Replicon | chromosome |
| Accession | NC_002754 | ||
| Organism | Sulfolobus solfataricus P2 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | Q97WZ2 |
| Locus tag | SSO_RS09575 | Protein ID | WP_009991912.1 |
| Coordinates | 1782460..1782846 (-) | Length | 129 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | Q97WZ1 |
| Locus tag | SSO_RS09580 | Protein ID | WP_009991915.1 |
| Coordinates | 1782833..1783108 (-) | Length | 92 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SSO_RS09545 (SSO1962) | 1777542..1778315 | - | 774 | WP_009991748.1 | extradiol dioxygenase | - |
| SSO_RS09550 (SSO1963) | 1778571..1778996 | - | 426 | WP_009991747.1 | DUF4143 domain-containing protein | - |
| SSO_RS09555 (SSO1964) | 1779283..1779639 | + | 357 | Protein_1907 | IS607 family transposase | - |
| SSO_RS09560 (SSO1966) | 1779715..1780665 | - | 951 | WP_010923701.1 | IS110 family transposase | - |
| SSO_RS09565 (SSO9368) | 1780824..1781108 | + | 285 | Protein_1909 | IS607-like element ISC1913 family transposase | - |
| SSO_RS09570 (SSO1967) | 1781092..1782312 | + | 1221 | WP_014511730.1 | IS200/IS605 family accessory protein TnpB-related protein | - |
| SSO_RS09575 (SSO1968) | 1782460..1782846 | - | 387 | WP_009991912.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SSO_RS09580 (SSO9378) | 1782833..1783108 | - | 276 | WP_009991915.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| SSO_RS09585 | 1783479..1783715 | + | 237 | WP_009991916.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| SSO_RS09590 (SSO1969) | 1783693..1784091 | + | 399 | WP_009991918.1 | type II toxin-antitoxin system VapC family toxin | - |
| SSO_RS09595 | 1784480..1784704 | + | 225 | WP_009991920.1 | type II toxin-antitoxin system CcdA family antitoxin | - |
| SSO_RS09600 (SSO1970) | 1784694..1785086 | + | 393 | WP_009991922.1 | type II toxin-antitoxin system VapC family toxin | - |
| SSO_RS09605 | 1785260..1786205 | + | 946 | Protein_1917 | IS110 family transposase | - |
| SSO_RS09610 (SSO1974) | 1786309..1787457 | - | 1149 | WP_010923706.1 | RNA-guided endonuclease TnpB family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | IScluster/Tn | - | - | 1779283..1791979 | 12696 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 129 a.a. Molecular weight: 15042.69 Da Isoelectric Point: 4.9844
>T951 WP_009991912.1 NC_002754:c1782846-1782460 [Saccharolobus solfataricus P2]
MIFLDANFLIYLNSGVSEVKEYYIKLLTYESLFSDPLVIDEVIYVSKKKYGVKYCDTIEFLDEIVLKYLTVLPITIKEYE
RAKEIMRKYSVKPSDAFHIAVMLNNSINVILSEDKELDKVAEIKRIWI
MIFLDANFLIYLNSGVSEVKEYYIKLLTYESLFSDPLVIDEVIYVSKKKYGVKYCDTIEFLDEIVLKYLTVLPITIKEYE
RAKEIMRKYSVKPSDAFHIAVMLNNSINVILSEDKELDKVAEIKRIWI
Download Length: 387 bp
>T951 NC_002754:c1782846-1782460 [Saccharolobus solfataricus P2]
TTGATTTTTCTTGATGCTAATTTCTTAATCTACTTAAACTCGGGTGTAAGTGAGGTAAAAGAGTATTATATAAAACTCCT
CACATATGAAAGCTTGTTTTCAGATCCTTTAGTTATAGATGAGGTAATATACGTGTCTAAGAAGAAGTATGGAGTTAAGT
ATTGCGATACCATAGAATTCTTGGATGAAATAGTGCTCAAGTACTTAACAGTTTTACCAATCACGATAAAGGAATATGAG
AGGGCTAAAGAAATTATGAGAAAATACTCAGTTAAGCCATCTGATGCCTTTCATATTGCCGTAATGTTAAACAATTCAAT
AAACGTGATCTTAAGTGAAGATAAGGAGTTAGATAAAGTTGCAGAAATAAAGAGAATATGGATTTAA
TTGATTTTTCTTGATGCTAATTTCTTAATCTACTTAAACTCGGGTGTAAGTGAGGTAAAAGAGTATTATATAAAACTCCT
CACATATGAAAGCTTGTTTTCAGATCCTTTAGTTATAGATGAGGTAATATACGTGTCTAAGAAGAAGTATGGAGTTAAGT
ATTGCGATACCATAGAATTCTTGGATGAAATAGTGCTCAAGTACTTAACAGTTTTACCAATCACGATAAAGGAATATGAG
AGGGCTAAAGAAATTATGAGAAAATACTCAGTTAAGCCATCTGATGCCTTTCATATTGCCGTAATGTTAAACAATTCAAT
AAACGTGATCTTAAGTGAAGATAAGGAGTTAGATAAAGTTGCAGAAATAAAGAGAATATGGATTTAA
Antitoxin
Download Length: 92 a.a. Molecular weight: 10372.22 Da Isoelectric Point: 7.0185
>AT951 WP_009991915.1 NC_002754:c1783108-1782833 [Saccharolobus solfataricus P2]
MSLTLRVEVGKKGYIIIPKSVRDLVGIKEGDILILTVVGDKIILEPERKVNIREVVKKLEEHERKISYAKRASLGELENI
SLEEELKIDFS
MSLTLRVEVGKKGYIIIPKSVRDLVGIKEGDILILTVVGDKIILEPERKVNIREVVKKLEEHERKISYAKRASLGELENI
SLEEELKIDFS
Download Length: 276 bp
>AT951 NC_002754:c1783108-1782833 [Saccharolobus solfataricus P2]
ATGAGTTTGACTCTAAGAGTGGAAGTTGGAAAGAAGGGTTATATAATAATTCCTAAAAGTGTAAGGGATTTGGTGGGAAT
AAAAGAGGGGGATATCTTAATTTTGACAGTCGTTGGAGATAAGATAATCTTAGAACCTGAGAGGAAGGTTAACATACGAG
AAGTTGTCAAAAAATTAGAAGAACATGAGAGGAAGATATCTTATGCTAAAAGGGCAAGCCTTGGGGAACTTGAGAACATA
AGTCTTGAAGAGGAATTGAAGATTGATTTTTCTTGA
ATGAGTTTGACTCTAAGAGTGGAAGTTGGAAAGAAGGGTTATATAATAATTCCTAAAAGTGTAAGGGATTTGGTGGGAAT
AAAAGAGGGGGATATCTTAATTTTGACAGTCGTTGGAGATAAGATAATCTTAGAACCTGAGAGGAAGGTTAACATACGAG
AAGTTGTCAAAAAATTAGAAGAACATGAGAGGAAGATATCTTATGCTAAAAGGGCAAGCCTTGGGGAACTTGAGAACATA
AGTCTTGAAGAGGAATTGAAGATTGATTTTTCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT956 | Sulfolobus solfataricus P2 |
34.247 |
100 |
0.342 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E3KDP7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E3MDU1 |
References
(1) Charlotte R Cooper et al. (2009) Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus. Biochemical Society Transactions 37(Pt 1):123-6. [PubMed:19143615]
(2) Sabrina Tachdjian et al. (2006) Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 188(12):4553-9. [PubMed:16740961]