Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/AbrB-PIN |
| Location | 2833113..2833719 | Replicon | chromosome |
| Accession | NC_002754 | ||
| Organism | Sulfolobus solfataricus P2 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | Q97UE3 |
| Locus tag | SSO_RS14920 | Protein ID | WP_009989090.1 |
| Coordinates | 2833113..2833517 (-) | Length | 135 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | M9UBQ9 |
| Locus tag | SSO_RS14925 | Protein ID | WP_009989091.1 |
| Coordinates | 2833498..2833719 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SSO_RS14895 (SSO3073) | 2828615..2829376 | - | 762 | WP_009990867.1 | ZIP family metal transporter | - |
| SSO_RS14900 | 2829524..2829706 | - | 183 | WP_009990866.1 | hypothetical protein | - |
| SSO_RS14905 (SSO3074) | 2830542..2831321 | + | 780 | WP_014511459.1 | DMT family transporter | - |
| SSO_RS14910 | 2831759..2832483 | + | 725 | Protein_2988 | transposase | - |
| SSO_RS17225 | 2832481..2832588 | - | 108 | Protein_2989 | IS110 family transposase | - |
| SSO_RS15820 (SSO11909) | 2832708..2832917 | + | 210 | Protein_2990 | transposase | - |
| SSO_RS14920 (SSO3078) | 2833113..2833517 | - | 405 | WP_009989090.1 | PIN domain-containing protein | Toxin |
| SSO_RS14925 (SSO11914) | 2833498..2833719 | - | 222 | WP_009989091.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| SSO_RS17230 (SSO11915) | 2833968..2834108 | + | 141 | WP_010924124.1 | hypothetical protein | - |
| SSO_RS14930 (SSO11917) | 2834248..2834448 | + | 201 | WP_009989095.1 | hypothetical protein | - |
| SSO_RS14935 (SSO11920) | 2834502..2834732 | + | 231 | WP_009989096.1 | EamA family transporter | - |
| SSO_RS14940 (SSO3079) | 2834843..2836114 | + | 1272 | WP_009989097.1 | MFS transporter | - |
| SSO_RS14945 (SSO3080) | 2836233..2836898 | + | 666 | WP_009989098.1 | helix-turn-helix domain-containing protein | - |
| SSO_RS14950 (SSO3081) | 2836895..2837281 | - | 387 | WP_009989099.1 | hypothetical protein | - |
| SSO_RS14955 (SSO3082) | 2837278..2837895 | - | 618 | WP_009989100.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 135 a.a. Molecular weight: 15426.98 Da Isoelectric Point: 5.0272
>T956 WP_009989090.1 NC_002754:c2833517-2833113 [Saccharolobus solfataricus P2]
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS
VEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNV
TDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS
Download Length: 405 bp
>T956 NC_002754:c2833517-2833113 [Saccharolobus solfataricus P2]
GTGGAGAAGGCAATAGTTGACACTAACGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCGGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTGGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATATTAGAAGTGAGAAAGCTGAGGTTGTCTGCGATTGTACAGATAACGTC
ACTGACGCTATAGGAGTGCTGAATAAGGAAAAGCTTCCCTTGGTCAGCTACAAAGATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGAAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA
GTGGAGAAGGCAATAGTTGACACTAACGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCGGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTGGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATATTAGAAGTGAGAAAGCTGAGGTTGTCTGCGATTGTACAGATAACGTC
ACTGACGCTATAGGAGTGCTGAATAAGGAAAAGCTTCCCTTGGTCAGCTACAAAGATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGAAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8267.50 Da Isoelectric Point: 9.6908
>AT956 WP_009989091.1 NC_002754:c2833719-2833498 [Saccharolobus solfataricus P2]
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS
MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS
Download Length: 222 bp
>AT956 NC_002754:c2833719-2833498 [Saccharolobus solfataricus P2]
ATGGAGAGGGTAAAGGTTACGAGAAATTACCAGATAACTATTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGG
AGATATACTTGAGGTTTACTTGAACGGTGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAG
GCAGTAAACTCTCACTGGGAGATATTGAGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA
ATGGAGAGGGTAAAGGTTACGAGAAATTACCAGATAACTATTCCAGCCTCGGCTAGGAGTAAGATTAATATAAAAGAAGG
AGATATACTTGAGGTTTACTTGAACGGTGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAG
GCAGTAAACTCTCACTGGGAGATATTGAGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T947 | Sulfolobus solfataricus P2 |
36.364 |
96.032 |
0.349 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT947 | Sulfolobus solfataricus P2 |
50 |
93.151 |
0.466 |
| AT951 | Sulfolobus solfataricus P2 |
34.247 |
100 |
0.342 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E3K068 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | M9UBQ9 |
References
(1) Charlotte R Cooper et al. (2009) Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus. Biochemical Society Transactions 37(Pt 1):123-6. [PubMed:19143615]
(2) Sabrina Tachdjian et al. (2006) Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic archaeon Sulfolobus solfataricus. Journal of Bacteriology 188(12):4553-9. [PubMed:16740961]