Detailed information of TA system TA080498

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4475748..4476160
• Replicon: chromosome
• Accession: NZ_CP023142
• Organism: Escherichia coli strain CFSAN061770

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: CA696_RS23265
• Protein ID: WP_000132601.1
• Coordinates: 4475819..4476160 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4475748..4475824 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA696_RS23255	4472611..4474200	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
CA696_RS23260	4474197..4475591	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
-	4475748..4475824	-	77	NuclAT_12	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_12	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_12	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_12	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_13	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_13	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_13	-	Antitoxin
-	4475748..4475824	-	77	NuclAT_13	-	Antitoxin
CA696_RS23265	4475819..4476160	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
CA696_RS23270	4476322..4476621	+	300	Protein_4285	DUF3578 domain-containing protein	-
CA696_RS23275	4476767..4477747	-	981	WP_000019402.1	IS5-like element IS5 family transposase	-
CA696_RS23280	4477782..4478900	+	1119	WP_095597211.1	AAA family ATPase	-
CA696_RS23285	4478900..4479946	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 IS/Tn TA006348 3666255..3667235 0.002 View NC_000913 Escherichia coli str. K-12 substr. MG1655 IS/Tn TA006364 3652182..3653162 0.002	flank	IS/Tn	-	-	4476767..4477747	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T80498 WP_000132601.1 NZ_CP023142:4475819-4476160 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T80498 NZ_CP023142:4475819-4476160 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT80498 NZ_CP023142:c4475824-4475748 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin