Detailed information of TA system TA006373

Experimentally validated

Overview

TA module

• Type: III
• Classification (family/domain): cptIN/-
• Location: 1348793..1349379
• Replicon: draft genome
• Accession: NC_021015
• Organism: Ruminococcus torques L2-14

Toxin (Protein)

• Gene name: cptN
• Uniprot ID: D4M3Y6
• Locus tag: RTO_13160
• Protein ID: WP_015528542.1
• Coordinates: 1348793..1349281 (-)
• Length: 163 a.a.

Antitoxin (RNA)

Gene name	cptI
Locus tag	-
Coordinates	1349334..1349379 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RTO_RS06270 (RTO_13110)	1344088..1344591	+	504	WP_015528539.1	flavodoxin	-
RTO_RS06275 (RTO_13120)	1344717..1345271	+	555	WP_015528540.1	flavin reductase	-
RTO_RS06280	1345287..1346054	+	768	WP_072521411.1	carboxymuconolactone decarboxylase family protein	-
RTO_RS06285 (RTO_13140)	1346117..1348369	-	2253	WP_015528541.1	tetratricopeptide repeat protein	-
RTO_RS15805 (RTO_13150)	1348619..1348753	+	135	WP_004844681.1	hypothetical protein	-
RTO_RS06290 (RTO_13160)	1348793..1349281	-	489	WP_015528542.1	hypothetical protein	Toxin
-	1349334..1349379	-	46	-	-	Antitoxin
RTO_RS06295 (RTO_13170)	1349570..1351255	+	1686	WP_015528543.1	recombinase family protein	-
RTO_RS06300 (RTO_13180)	1351566..1352327	+	762	WP_015528544.1	helix-turn-helix transcriptional regulator	-
RTO_RS06305 (RTO_13200)	1352763..1353110	+	348	Protein_1253	YjdF family protein	-
RTO_RS06310 (RTO_13210)	1353188..1353568	+	381	WP_015528547.1	DUF4825 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1309616..1383396	73780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 19196.16 Da
• Isoelectric Point: 9.4263

>T6373 WP_015528542.1 NC_021015:c1349281-1348793 [[Ruminococcus] torques L2-14]
MIYQEGYVYHIKDEYFEKVQDSNLMQNKEGGTYRPTFYCLRDNKTSLLWMVPLSSRVEKFKAIHDKQVAKYGKCLTIVLG
EFDGKEAAFLLQNMFPIREYYLDHIHTRNNNPVPVKHSIHREVTTRMKKIRQLHSRGKKVVFPDIDRLEQIMLAEVKDNA
TE

Nucleotide

• Length: 489 bp

>T6373 NC_021015:c1349281-1348793 [[Ruminococcus] torques L2-14]
ATGATATATCAAGAAGGATACGTTTACCATATTAAAGATGAATACTTTGAAAAAGTACAGGATTCCAACTTAATGCAGAA
CAAAGAGGGCGGTACATACCGTCCTACTTTTTACTGTCTGCGTGACAATAAAACTTCTCTGTTATGGATGGTTCCACTCA
GTTCTCGTGTAGAAAAGTTTAAAGCAATACACGATAAACAGGTGGCTAAATACGGAAAGTGCCTAACGATTGTTTTAGGT
GAATTTGATGGAAAAGAAGCTGCTTTTCTTCTCCAGAATATGTTTCCAATCAGAGAGTACTATCTTGACCATATACATAC
TCGAAATAATAATCCGGTTCCTGTTAAGCATTCCATTCACAGGGAAGTAACAACACGTATGAAAAAAATTCGTCAACTTC
ATTCCAGAGGCAAAAAAGTGGTATTTCCAGATATTGACCGACTAGAGCAGATTATGCTTGCAGAAGTAAAAGACAACGCA
ACCGAATAA

Antitoxin

Nucleotide

• Length: 46 bp

>AT6373 NC_021015:c1349379-1349334 [[Ruminococcus] torques L2-14]
TCGAAAATATTGTTCGCTCACCGGAAGCGTCTAATAAATGTCCGGC

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6372	Coprococcus catus GD/7	43.506	96.25	0.419
T6374	Eubacterium rectale DSM 17629	34.839	95.679	0.333

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A6L8T7Y6

Antitoxin

References

(1) Tim R Blower et al. (2012) Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes. Nucleic Acids Research 40(13):6158-73. [PubMed:22434880]


 experimental literature