Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Xre-RelE |
| Location | 4239649..4240403 | Replicon | chromosome |
| Accession | NC_003197 | ||
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | STM4031 | Protein ID | NP_462911.1 |
| Coordinates | 4240105..4240416 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | STM4030 | Protein ID | NP_462910.3 |
| Coordinates | 4239662..4240108 (-) | Length | 149 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| STM4024 | 4234838..4235734 | + | 897 | NP_462904.3 | putative sugar kinase | - |
| STM4025 | 4235768..4236571 | + | 804 | NP_462905.1 | putative glycerol-3-phosphate regulon repressor | - |
| STM4026 | 4236762..4237361 | + | 600 | NP_462906.1 | putative enzyme | - |
| STM4027 | 4237355..4238227 | + | 873 | NP_462907.1 | tRNA processing exoribonuclease BN | - |
| STM4028 | 4238224..4238661 | + | 438 | NP_462908.1 | D-Tyr-tRNA(Tyr) deacylase | - |
| STM4029 | 4238658..4239647 | + | 990 | NP_462909.1 | putative acetyltransferase | - |
| STM4030 | 4239662..4240108 | - | 447 | NP_462910.3 | putative cytoplasmic protein | Antitoxin |
| STM4031 | 4240105..4240416 | - | 312 | NP_462911.1 | putative cytoplasmic protein | Toxin |
| STM4032 | 4240502..4241431 | - | 930 | NP_462912.1 | putative acetyl esterase | - |
| STM4033 | 4241997..4242251 | + | 255 | NP_462913.3 | putative merR family bacterial regulatory protein | - |
| STM4034 | 4242298..4243227 | - | 930 | NP_462914.1 | putative formate dehydrogenase formation protein | - |
| STM4035 | 4243224..4243859 | - | 636 | NP_462915.1 | formate dehydrogenase, cytochrome B556 (FDO) subunit | - |
| STM4036 | 4243856..4244758 | - | 903 | NP_462916.1 | formate dehydrogenase-O, Fe-S subunit | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12432.40 Da Isoelectric Point: 9.5334
>T6087 NP_462911.1 NC_003197:c4240416-4240105 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE
Download Length: 312 bp
>T6087 NC_003197:c4240416-4240105 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA
Antitoxin
Download Length: 149 a.a. Molecular weight: 16734.08 Da Isoelectric Point: 6.6451
>AT6087 NP_462910.3 NC_003197:c4240108-4239662 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE
Download Length: 447 bp
>AT6087 NC_003197:c4240108-4239662 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
40.404 |
96.117 |
0.388 |
| T10135 | Shigella flexneri 5a str. M90T |
40.404 |
96.117 |
0.388 |
| T1046 | Escherichia coli str. K-12 substr. MG1655 |
40.404 |
96.117 |
0.388 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1046 | Escherichia coli str. K-12 substr. MG1655 |
45.902 |
88.406 |
0.406 |
| AT10135 | Shigella flexneri 5a str. M90T |
45.902 |
88.406 |
0.406 |
| AT10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
45.902 |
88.406 |
0.406 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Miguel A De la Cruz et al. (2013) A toxin-antitoxin module of Salmonella promotes virulence in mice. PLoS Pathogens 9(12):e1003827. [PubMed:24385907]
(2) Fernando Chimal-Cázares et al. (2020) Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence. Frontiers in Microbiology 11:614. [PubMed:32328049]