Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 13361..14088 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EKN05_RS00070 Protein ID WP_000550189.1
Coordinates 13361..13675 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EKN05_RS00075 Protein ID WP_000560266.1
Coordinates 13672..14088 (+) Length 139 a.a.

Genomic Context

EKN05_RS00065EKN05_RS00070EKN05_RS00075EKN05_RS00050EKN05_RS00055EKN05_RS00060EKN05_RS00080EKN05_RS00085
Location: 11940..13076 (1137 bp)
Type: Others
Protein ID: WP_000018658.1
Location: 13361..13675 (315 bp)
Type: Toxin
Protein ID: WP_000550189.1
Location: 13672..14088 (417 bp)
Type: Antitoxin
Protein ID: WP_000560266.1
Location: 9506..10504 (999 bp)
Type: Others
Protein ID: WP_005050984.1
Location: 10583..11275 (693 bp)
Type: Others
Protein ID: WP_000942537.1
Location: 11352..11855 (504 bp)
Type: Others
Protein ID: Protein_10
Location: 14133..16151 (2019 bp)
Type: Others
Protein ID: WP_000121485.1
Location: 16377..18728 (2352 bp)
Type: Others
Protein ID: Protein_15
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
EKN05_RS00050 (EKN05_000050)9506..10504-999WP_005050984.1Gfo/Idh/MocA family oxidoreductase-
EKN05_RS00055 (EKN05_000055)10583..11275-693WP_000942537.1vancomycin high temperature exclusion protein-
EKN05_RS00060 (EKN05_000060)11352..11855-504Protein_10M48 family metallopeptidase-
EKN05_RS00065 (EKN05_000065)11940..13076+1137WP_000018658.123S rRNA (guanine(1835)-N(2))-methyltransferase RlmG-
EKN05_RS00070 (EKN05_000070)13361..13675+315WP_000550189.1type II toxin-antitoxin system toxin HigBToxin
EKN05_RS00075 (EKN05_000075)13672..14088+417WP_000560266.1type II toxin-antitoxin system antitoxin HigAAntitoxin
EKN05_RS00080 (EKN05_000080)14133..16151-2019WP_000121485.1NADPH-dependent 2,4-dienoyl-CoA reductase-
EKN05_RS00085 (EKN05_000085)16377..18728-2352Protein_15alpha-glucosidase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
No matching records found


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T10135 WP_000550189.1 NZ_CP037923:13361-13675 [Shigella flexneri 5a str. M90T]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T10135 NZ_CP037923:13361-13675 [Shigella flexneri 5a str. M90T]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT10135 WP_000560266.1 NZ_CP037923:13672-14088 [Shigella flexneri 5a str. M90T]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT10135 NZ_CP037923:13672-14088 [Shigella flexneri 5a str. M90T]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T1046Escherichia coli str. K-12 substr. MG1655

100

100

1

T10125Escherichia coli str. K-12 substr. MC4100 strain K-12

100

100

1

T6087Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

40.404

96.117

0.388
Showing 1 to 3 of 3 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT10125Escherichia coli str. K-12 substr. MC4100 strain K-12

100

100

1

AT1046Escherichia coli str. K-12 substr. MG1655

100

100

1

AT6087Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

45.902

88.406

0.406
Showing 1 to 3 of 3 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
PDB6KMQ
PDB5IFG
PDB5YCL
PDB6KML

Antitoxin

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Source
ID
Structure
No matching records found

References

(1) Won-Su Yoon et al. (2019) Structural changes of antitoxin HigA from Shigella flexneri by binding of its cognate toxin HigB. International Journal of Biological Macromolecules 130:99-108. [PubMed:30797012] experimental


experimental experimental literature