Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/- |
| Location | 13361..14088 | Replicon | chromosome |
| Accession | NZ_CP037923 | ||
| Organism | Shigella flexneri 5a str. M90T | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U9ZMR4 |
| Locus tag | EKN05_RS00070 | Protein ID | WP_000550189.1 |
| Coordinates | 13361..13675 (+) | Length | 105 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | EKN05_RS00075 | Protein ID | WP_000560266.1 |
| Coordinates | 13672..14088 (+) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKN05_RS00050 (EKN05_000050) | 9506..10504 | - | 999 | WP_005050984.1 | Gfo/Idh/MocA family oxidoreductase | - |
| EKN05_RS00055 (EKN05_000055) | 10583..11275 | - | 693 | WP_000942537.1 | vancomycin high temperature exclusion protein | - |
| EKN05_RS00060 (EKN05_000060) | 11352..11855 | - | 504 | Protein_10 | M48 family metallopeptidase | - |
| EKN05_RS00065 (EKN05_000065) | 11940..13076 | + | 1137 | WP_000018658.1 | 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG | - |
| EKN05_RS00070 (EKN05_000070) | 13361..13675 | + | 315 | WP_000550189.1 | type II toxin-antitoxin system toxin HigB | Toxin |
| EKN05_RS00075 (EKN05_000075) | 13672..14088 | + | 417 | WP_000560266.1 | type II toxin-antitoxin system antitoxin HigA | Antitoxin |
| EKN05_RS00080 (EKN05_000080) | 14133..16151 | - | 2019 | WP_000121485.1 | NADPH-dependent 2,4-dienoyl-CoA reductase | - |
| EKN05_RS00085 (EKN05_000085) | 16377..18728 | - | 2352 | Protein_15 | alpha-glucosidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 12103.10 Da Isoelectric Point: 10.0409
>T10135 WP_000550189.1 NZ_CP037923:13361-13675 [Shigella flexneri 5a str. M90T]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
Download Length: 315 bp
>T10135 NZ_CP037923:13361-13675 [Shigella flexneri 5a str. M90T]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 139 a.a. Molecular weight: 14995.42 Da Isoelectric Point: 4.4547
>AT10135 WP_000560266.1 NZ_CP037923:13672-14088 [Shigella flexneri 5a str. M90T]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
Download Length: 417 bp
>AT10135 NZ_CP037923:13672-14088 [Shigella flexneri 5a str. M90T]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1046 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| T10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
100 |
100 |
1 |
| T6087 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
40.404 |
96.117 |
0.388 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
100 |
100 |
1 |
| AT1046 | Escherichia coli str. K-12 substr. MG1655 |
100 |
100 |
1 |
| AT6087 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
45.902 |
88.406 |
0.406 |
Multiple sequence alignment
References
(1) Won-Su Yoon et al. (2019) Structural changes of antitoxin HigA from Shigella flexneri by binding of its cognate toxin HigB. International Journal of Biological Macromolecules 130:99-108. [PubMed:30797012]