Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Xre-RelE |
| Location | 3231750..3232477 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | P64578 |
| Locus tag | b3083 | Protein ID | NP_417554.1 |
| Coordinates | 3234141..3234455 (-) | Length | 105 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | P67701 |
| Locus tag | b3082 | Protein ID | NP_417553.1 |
| Coordinates | 3233728..3234144 (-) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b3079 | 3227801..3228871 | + | 1071 | NP_417550.1 | protein YgjJ | - |
| b3080 | 3228888..3231239 | + | 2352 | NP_417551.1 | glycoside hydrolase | - |
| b3081 | 3231665..3233683 | + | 2019 | NP_417552.1 | 2,4-dienoyl-CoA reductase | - |
| b3082 | 3233728..3234144 | - | 417 | NP_417553.1 | antitoxin/DNA-binding transcriptional repressor HigA | Antitoxin |
| b3083 | 3234141..3234455 | - | 315 | NP_417554.1 | ribosome-dependent mRNA interferase toxin HigB | Toxin |
| b3084 | 3234739..3235875 | - | 1137 | NP_417555.4 | 23S rRNA m(2)G1835 methyltransferase | - |
| b3085 | 3235960..3236463 | + | 504 | NP_417556.2 | putative metal-dependent hydrolase | - |
| b3086 | 3236540..3237232 | + | 693 | NP_417557.1 | DUF218 domain-containing protein YgjQ | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 12103.10 Da Isoelectric Point: 10.0409
>T1046 NP_417554.1 NC_000913:c3234455-3234141 [Escherichia coli str. K-12 substr. MG1655]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
Download Length: 315 bp
>T1046 NC_000913:c3234455-3234141 [Escherichia coli str. K-12 substr. MG1655]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 139 a.a. Molecular weight: 14995.42 Da Isoelectric Point: 4.4547
>AT1046 NP_417553.1 NC_000913:c3234144-3233728 [Escherichia coli str. K-12 substr. MG1655]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
Download Length: 417 bp
>AT1046 NC_000913:c3234144-3233728 [Escherichia coli str. K-12 substr. MG1655]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
100 |
100 |
1 |
| T10135 | Shigella flexneri 5a str. M90T |
100 |
100 |
1 |
| T6087 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
40.404 |
96.117 |
0.388 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10135 | Shigella flexneri 5a str. M90T |
100 |
100 |
1 |
| AT10125 | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
100 |
100 |
1 |
| AT6087 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
45.902 |
88.406 |
0.406 |
Multiple sequence alignment
References
(1) Pankaj Vilas Jadhav et al. (2020) 2.09 Å Resolution structure of E. coli HigBA toxin-antitoxin complex reveals an ordered DNA-binding domain and intrinsic dynamics in antitoxin. The Biochemical Journal 477(20):4001-4019. [PubMed:33000860]
(2) J Paul Norton et al. (2012) Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli. PLoS Pathogens 8(10):e1002954. [PubMed:23055930]
(3) Mikkel Christensen-Dalsgaard et al. (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Molecular Microbiology 75(2):333-48. [PubMed:19943910]