33222

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 2073986..2074499 Replicon chromosome
Accession NZ_AP020310
Organism Streptococcus agalactiae strain GCMC97051

Toxin (Protein)


Gene name yoeB Uniprot ID Q8DX49
Locus tag SAGCMC97051_RS10690 Protein ID WP_000481712.1
Coordinates 2073986..2074240 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q8DX48
Locus tag SAGCMC97051_RS10695 Protein ID WP_000387490.1
Coordinates 2074233..2074499 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAGCMC97051_RS10670 2071207..2072011 - 805 Protein_2035 IS5 family transposase -
SAGCMC97051_RS10675 (SAGCMC97051_20360) 2072272..2072682 - 411 WP_000390747.1 conjugal transfer protein -
SAGCMC97051_RS10680 (SAGCMC97051_20370) 2072693..2072959 - 267 WP_001055037.1 hypothetical protein -
SAGCMC97051_RS10685 (SAGCMC97051_20380) 2072980..2073933 - 954 WP_000565540.1 conjugal transfer protein -
SAGCMC97051_RS10690 (SAGCMC97051_20390) 2073986..2074240 - 255 WP_000481712.1 Txe/YoeB family addiction module toxin Toxin
SAGCMC97051_RS10695 (SAGCMC97051_20400) 2074233..2074499 - 267 WP_000387490.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
SAGCMC97051_RS10700 (SAGCMC97051_20410) 2074644..2075117 - 474 WP_000560386.1 hypothetical protein -
SAGCMC97051_RS10705 (SAGCMC97051_20420) 2075125..2075607 - 483 WP_000383283.1 antirestriction protein ArdA -
SAGCMC97051_RS10710 (SAGCMC97051_20430) 2075660..2075932 - 273 WP_000017154.1 hypothetical protein -
SAGCMC97051_RS10715 (SAGCMC97051_20440) 2076067..2076636 - 570 WP_000010056.1 hypothetical protein -
SAGCMC97051_RS10720 (SAGCMC97051_20450) 2076639..2077988 - 1350 WP_000491712.1 AAA family ATPase -
SAGCMC97051_RS10725 (SAGCMC97051_20460) 2078185..2078484 - 300 WP_000737133.1 helix-turn-helix transcriptional regulator -
SAGCMC97051_RS10730 (SAGCMC97051_20470) 2078468..2078845 - 378 WP_000287183.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2061246..2091191 29945
flank IS/Tn - - 2071207..2071596 389


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-83)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10445.87 Da        Isoelectric Point: 6.7282

>T33222 WP_000481712.1 NZ_AP020310:c2074240-2073986 [Streptococcus agalactiae]
MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKPEPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFR
DHYK

Download         Length: 255 bp

>T33222 NZ_AP020310:c2074240-2073986 [Streptococcus agalactiae]
ATGTTTAATTTTACTGAAGAAGCATGGAAAGATTATGTTTCTTGGCAACAGGAAGATAAAAAAATCTTGAAACGAATTAA
TCGTTTGATAGAAGATATTAAAAGAGACCCATTTGAAGGCATTGGAAAACCAGAACCTTTAAAATACCATTATTCAGGGG
CTTGGTCTCGAAGAATTACAGAAGAACATAGGTTGATTTATATGATAGAAGATGGAGAAATCTATTTTTTATCTTTTAGA
GATCATTATAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10316.70 Da        Isoelectric Point: 4.6742

>AT33222 WP_000387490.1 NZ_AP020310:c2074499-2074233 [Streptococcus agalactiae]
MEAIVYSHFRNNLKDYMKKVNDEFEPLIVVNKNPDENIVVLSQDSWESLQETIRLMENDYLSHKVINGISQVKEKQVTKH
GLIEVEDV

Download         Length: 267 bp

>AT33222 NZ_AP020310:c2074499-2074233 [Streptococcus agalactiae]
ATGGAGGCTATTGTTTATTCACATTTTAGAAATAATTTAAAAGATTATATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GATTGTAGTTAATAAAAATCCTGATGAAAATATAGTGGTTTTATCTCAAGATAGCTGGGAAAGTTTACAAGAAACGATTC
GTTTGATGGAAAATGATTATCTATCACATAAGGTTATTAATGGTATATCACAAGTTAAAGAGAAGCAAGTCACTAAACAT
GGTTTAATTGAGGTAGAAGATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IC12

Antitoxin

Source ID Structure
AlphaFold DB A0A829I9V6

References