Detailed information of TA system TA024439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3234400..3236120
• Replicon: chromosome
• Accession: NC_013508
• Organism: Edwardsiella tarda EIB202

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: D0ZER0
• Locus tag: ETAE_RS14270
• Protein ID: WP_012849887.1
• Coordinates: 3234400..3235056 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: ETAE_RS14275
• Protein ID: WP_012849888.1
• Coordinates: 3235053..3236120 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETAE_RS14255 (ETAE_3068)	3229764..3230003	+	240	WP_012849883.1	hypothetical protein	-
ETAE_RS14260 (ETAE_3069)	3230042..3232480	+	2439	WP_012849884.1	type I restriction-modification system subunit M	-
ETAE_RS14265 (ETAE_3070)	3232492..3233817	+	1326	WP_012849885.1	restriction endonuclease subunit S	-
ETAE_RS17590 (ETAE_3071)	3233822..3234397	+	576	WP_012849886.1	DUF4433 domain-containing protein	-
ETAE_RS14270 (ETAE_3072)	3234400..3235056	+	657	WP_012849887.1	DUF4433 domain-containing protein	Toxin
ETAE_RS14275 (ETAE_3073)	3235053..3236120	+	1068	WP_012849888.1	macro domain-containing protein	Antitoxin
ETAE_RS14280 (ETAE_3074)	3236284..3239379	+	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
ETAE_RS14285 (ETAE_3075)	3239379..3240095	+	717	WP_012849890.1	M48 family metallopeptidase	-
ETAE_RS14290 (ETAE_3076)	3240340..3240807	+	468	Protein_2846	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_LC127084 Edwardsiella tarda strain ET-1 Prophage TA285378, TA285379 2595957..2651606 0	inside	Prophage	-	-	3201940..3257588	55648
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_LC127084 Edwardsiella tarda strain ET-1 IS/Tn TA285378, TA285379 2634910..2635791 0	flank	IS/Tn	-	-	3240893..3241774	881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25146.59 Da
• Isoelectric Point: 6.7624

>T24439 WP_012849887.1 NC_013508:3234400-3235056 [Edwardsiella tarda EIB202]
VAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T24439 NC_013508:3234400-3235056 [Edwardsiella tarda EIB202]
GTGGCCTACGACTACAGCGCCAACCTCAACCCGGAAAAAGCCCTGATTTGGCGCATTGTCCATCGGGATAATATCCCGTG
GATACTGGACCACGGTCTGCACTGCGGCAACAGCCCGGTGCAGGCGGCAAACTGGATCAATATCGGCAACCCGGAACTTA
TCGGCAAACGCGCCGGGCATCCGGTGCCTGTCGGTATGGGCGGGACGCTGCATGACTACGTGCCGTTTTACTTTACCCCC
TTTTCGCCGATGCTGATGAATATCCACAGCGGGCGTGGCGGCATTACGCAGCGCCCCAATGAGGAAATCGTCATTCTGGT
CAGCAACCTGCGAAACGTGGCGGCGCAGAACATCCCCTTCGTGTTTACCGACAGCCACGCCTACTATAACTGGGTCAACT
ACTACACCCGTCTGGACGATCTGAACCAGATCGACTGGTCTATCTTACAGGCGCGGGATTTCCGTCGCGATCCAGACGAT
CCGGCGAAGTTTGAACGCTATCAGGCCGAGGCGTTAATCTGGCAGCACTGCCCCATCACACTGCTGGTCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGCTGTCGCAACGTAATCTGACAATGCCCGTTCACGCACGGGCCG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40713.02 Da
• Isoelectric Point: 6.9067

>AT24439 WP_012849888.1 NC_013508:3235053-3236120 [Edwardsiella tarda EIB202]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESTLGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT24439 NC_013508:3235053-3236120 [Edwardsiella tarda EIB202]
ATGATCACATATACACAAGGCAATTTACTGGACGCGCCTGTGGAAGCACTGGTGAATACGGTGAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTTAAAGAGCGCTTCCCCGACAATATGAAAGCCTATGCCCACGCCTGCAAACAAAAGC
AGGTGATGACCGGCAAGATGTTTATTACCGAAACCGGCGAGCTGATGGGGCCACGCTGGATCGTCAACTTCCCTACCAAG
CAGCACTGGCGCGCCGACTCCCGCATGGCGTGGATCGAAGACGGTTTGCAGGACTTGCGCCGCTTTTTGATCGAAAAGAA
AGTGCGGTCCATCGCCATTCCCCCGCTGGGCGCGGGCAACGGCGGCCTGAACTGGCCGGACGTCCGGGCGCGGATTGAAT
CTACGCTGGGCGACCTTCAGGACACGGAAATCCTGATTTATGAGCCTACCGAAAAATACCAGAATGTCGCCAAGAGCAGC
GGTGTAAAAAAGCTCACGCCCGCCAGAGCGATGATCGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGTATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGCTACAACGCGCCATTGAACAGCACCAGCAGGAGGACGTGTTAAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTATGCTCCTAACCTGAACCATCTGCTTAACGCGCTGGACGGTACCTATCTGAAA
GCCGAGAAACGTATCCCGGACAGCCAGCCACTGGATGTGATCTGGTTTAACGATCGGGAAAAAGAACACATCAACCTATG
GCTGAACAGCGAAGCCAAAGCGTGGTTGCCAGCGCTTGAGCAGGTCAGCCAGTTGATTGACGGTTTTGAATCGCCGTTTG
GTCTGGAGCTGTTGGCGACCGTTGACTGGTTGTTGACCCGGGGACAATGCCAGCCGACGCTGGACTCGGTGAAAGAGGGG
TTGCATCAGTGGCCTGCGGGAGAACCTTGGGCCAGCCGTAAGATAAAGCTGTTTGACGATAAGAATCTGCAATTCGCCAT
TAACCGCGTGATGGAGTTTCATTGCTGA

Structures

Toxin

• AlphaFold DB: D0ZER0