Detailed information of TA system TA000226

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH-RelE
• Location: 890690..891231
• Replicon: chromosome
• Accession: NC_002696
• Organism: Caulobacter crescentus CB15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9AA09
• Locus tag: CC_RS04185
• Protein ID: WP_010918687.1
• Coordinates: 890690..890971 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9AA08
• Locus tag: CC_RS04190
• Protein ID: WP_010918688.1
• Coordinates: 890968..891231 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CC_RS04170 (CC_0799)	885772..887388	-	1617	WP_012640065.1	carboxylesterase/lipase family protein	-
CC_RS04175 (CC_0800)	887478..889202	-	1725	WP_010918685.1	hypothetical protein	-
CC_RS04180 (CC_0801)	889286..890614	-	1329	WP_010918686.1	mannanase	-
CC_RS04185 (CC_0802)	890690..890971	-	282	WP_010918687.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CC_RS04190 (CC_0803)	890968..891231	-	264	WP_010918688.1	type II toxin-antitoxin system antitoxin RelB1	Antitoxin
CC_RS04195 (CC_0804)	891270..894359	-	3090	WP_010918689.1	bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA	-
CC_RS04200	894444..894605	+	162	WP_024265629.1	hypothetical protein	-
CC_RS04205 (CC_0805)	894805..895272	+	468	WP_010918690.1	Lrp/AsnC ligand binding domain-containing protein	-
CC_RS04210	895299..895463	-	165	WP_024265630.1	leucine-responsive regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10486.14 Da
• Isoelectric Point: 11.7926

>T226 WP_010918687.1 NC_002696:c890971-890690 [Caulobacter vibrioides CB15]
MTFTVLVSVRAKRDFNRLIVWLVERDPRAAARLGPLLEAALDSLTEAPSRGRSVGPTTREISIPFGQSAYVIRYRLLGSS
VHVTRIWHGLEQR

Nucleotide

• Length: 282 bp

>T226 NC_002696:c890971-890690 [Caulobacter vibrioides CB15]
TTGACCTTCACGGTTTTGGTATCCGTTCGGGCCAAGAGAGACTTCAACCGTCTCATCGTCTGGCTTGTGGAGCGCGACCC
CCGAGCAGCGGCTAGACTGGGCCCACTTCTGGAGGCCGCCCTCGACAGTTTGACCGAGGCGCCATCGCGAGGTCGCTCGG
TTGGTCCCACCACCCGCGAGATCAGCATTCCTTTTGGTCAGAGCGCCTATGTGATCCGCTACCGGCTCCTCGGCTCCAGC
GTACATGTCACCCGGATCTGGCACGGCCTTGAGCAAAGGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9198.31 Da
• Isoelectric Point: 4.2560

>AT226 WP_010918688.1 NC_002696:c891231-890968 [Caulobacter vibrioides CB15]
MADGFDIHIDQEQAARLKVVADRLGMSVSEYAVALIDAGLTGAAPKAIDPDPAIDEAIADAIERGDEPAISRDEFRAHIR
RVTAGLG

Nucleotide

• Length: 264 bp

>AT226 NC_002696:c891231-890968 [Caulobacter vibrioides CB15]
ATGGCCGATGGCTTCGACATTCATATCGATCAAGAGCAGGCCGCCCGGCTGAAGGTCGTTGCCGACCGCCTGGGTATGTC
CGTGTCCGAGTACGCCGTTGCGCTGATCGACGCAGGGCTGACAGGCGCGGCGCCTAAAGCGATCGATCCGGACCCCGCCA
TCGACGAGGCCATCGCCGACGCCATCGAGCGGGGCGACGAGCCGGCGATTTCACGCGATGAGTTCCGCGCTCATATACGT
CGCGTGACTGCGGGCCTGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10142	Pseudomonas savastanoi	36.264	97.849	0.355
T229	Caulobacter crescentus CB15	37.5	86.022	0.323
T10137	Pseudomonas savastanoi	31.818	94.624	0.301

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q9AA09

Antitoxin

• AlphaFold DB: Q9AA08

References

(1) Aretha Fiebig et al. (2010) Interaction specificity, toxicity and regulation of a paralogous set of ParE/RelE-family toxin-antitoxin systems. Molecular Microbiology 77(1):236-51. [PubMed:20487277]


 experimental literature