Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 37068..37615 | Replicon | plasmid pPsv48A |
| Accession | NC_019265 | ||
| Organism | Pseudomonas savastanoi | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | H1ZWV0 |
| Locus tag | PSA3335_RS00175 | Protein ID | WP_015060661.1 |
| Coordinates | 37068..37343 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | H1ZWV1 |
| Locus tag | PSA3335_RS00180 | Protein ID | WP_015060662.1 |
| Coordinates | 37343..37615 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSA3335_RS00150 (PSA3335_A0027) | 33062..33658 | - | 597 | WP_015060659.1 | recombinase family protein | - |
| PSA3335_RS00155 | 33690..33881 | + | 192 | WP_168550531.1 | hypothetical protein | - |
| PSA3335_RS00160 (PSA3335_A0028) | 33932..34642 | - | 711 | WP_223227236.1 | XopAF/AvrXv3 family type III secretion system effector | - |
| PSA3335_RS00165 (PSA3335_A0029) | 35270..35521 | + | 252 | Protein_32 | helix-turn-helix domain-containing protein | - |
| PSA3335_RS00170 (PSA3335_A0030) | 35871..36905 | - | 1035 | WP_015060660.1 | IS110-like element ISPsy16 family transposase | - |
| PSA3335_RS00175 (PSA3335_A0031) | 37068..37343 | - | 276 | WP_015060661.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| PSA3335_RS00180 (PSA3335_A0032) | 37343..37615 | - | 273 | WP_015060662.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| PSA3335_RS00185 (PSA3335_A0034) | 38306..39528 | + | 1223 | WP_095178853.1 | IS3-like element IS51 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..80123 | 80123 | |
| - | inside | IScluster/Tn | - | - | 24464..39528 | 15064 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10751.33 Da Isoelectric Point: 7.0126
>T10137 WP_015060661.1 NC_019265:c37343-37068 [Pseudomonas savastanoi pv. savastanoi]
MELKWTSKALSDITRLYEFLALANQPAAARVVQQLTTAPTTLLTNPRIGERLEEFEPKDVRRIQIGQYEMRYEIADSTIY
LLRLWHTREDR
MELKWTSKALSDITRLYEFLALANQPAAARVVQQLTTAPTTLLTNPRIGERLEEFEPKDVRRIQIGQYEMRYEIADSTIY
LLRLWHTREDR
Download Length: 276 bp
>T10137 NC_019265:c37343-37068 [Pseudomonas savastanoi pv. savastanoi]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCAGGCTATACGAGTTTCTGGCCTTGGCGAATCAACCTGC
CGCAGCACGAGTAGTGCAGCAACTCACCACAGCACCTACCACGCTTCTGACTAACCCGCGCATTGGCGAACGCCTAGAGG
AGTTCGAGCCAAAGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCAACTATTTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCAGGCTATACGAGTTTCTGGCCTTGGCGAATCAACCTGC
CGCAGCACGAGTAGTGCAGCAACTCACCACAGCACCTACCACGCTTCTGACTAACCCGCGCATTGGCGAACGCCTAGAGG
AGTTCGAGCCAAAGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCAACTATTTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG
Antitoxin
Download Length: 91 a.a. Molecular weight: 10076.40 Da Isoelectric Point: 5.6994
>AT10137 WP_015060662.1 NC_019265:c37615-37343 [Pseudomonas savastanoi pv. savastanoi]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
STDTPLPVPR
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
STDTPLPVPR
Download Length: 273 bp
>AT10137 NC_019265:c37615-37343 [Pseudomonas savastanoi pv. savastanoi]
ATGGCAACCACCGCCAAAACCCGATCTGTCACAGCTCACGTTCCGGTTCAGCTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCTAAAAACTGGATCGTCAAGCAAGCCCTGTCTGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCTGATGTTGACGCTGGACGCGTTATTGATCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTT
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA
ATGGCAACCACCGCCAAAACCCGATCTGTCACAGCTCACGTTCCGGTTCAGCTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCTAAAAACTGGATCGTCAAGCAAGCCCTGTCTGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCTGATGTTGACGCTGGACGCGTTATTGATCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTT
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T226 | Caulobacter crescentus CB15 |
31.818 |
94.624 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A554FV80 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3M4CJB9 |
References
(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866]