Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 58390..59009 Replicon plasmid pTEF1
Accession NC_004669
Organism Enterococcus faecalis V583

Toxin (Protein)


Gene name mazF Uniprot ID R3H4V2
Locus tag EF_RS16455 Protein ID WP_000241511.1
Coordinates 58390..58752 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3H5D1
Locus tag EF_RS16460 Protein ID WP_000245205.1
Coordinates 58746..59009 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF_RS16440 (EF_A0067) 53800..55731 - 1932 WP_002370258.1 sucrose-specific PTS transporter subunit IIBC -
EF_RS16445 (EF_A0069) 55922..57361 + 1440 WP_002367771.1 sucrose-6-phosphate hydrolase -
EF_RS16450 (EF_A0070) 57363..58325 + 963 WP_002367770.1 LacI family DNA-binding transcriptional regulator -
EF_RS16455 (EF_A0071) 58390..58752 - 363 WP_000241511.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EF_RS16460 (EF_A0072) 58746..59009 - 264 WP_000245205.1 PbsX family transcriptional regulator Antitoxin
EF_RS16465 (EF_A0073) 59186..59806 + 621 WP_021732893.1 recombinase family protein -
EF_RS16470 (EF_A0074) 59823..60110 + 288 WP_010708491.1 hypothetical protein -
EF_RS16475 60104..60328 + 225 WP_010708492.1 ultraviolet resistance protein UvrA repressor UvrC -
EF_RS16480 (EF_A0075) 60389..60598 + 210 WP_002387638.1 hypothetical protein -
EF_RS16895 60760..60912 + 153 WP_225850000.1 DUF6440 family protein -
EF_RS16490 (EF_A0078) 61339..62667 + 1329 WP_002387639.1 ultraviolet resistance protein UvrA -
EF_RS16495 (EF_A0079) 62664..63014 + 351 WP_002365943.1 hypothetical protein -
EF_RS16900 (EF_A0080) 62971..63183 + 213 WP_002365945.1 hypothetical protein -
EF_RS16500 63274..63375 + 102 WP_002387930.1 type I toxin-antitoxin system Fst family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid erm(B) / qacZ / aac(6')-aph(2'') asa1 1..66320 66320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13585.78 Da        Isoelectric Point: 9.6107

>T20927 WP_000241511.1 NC_004669:c58752-58390 [Enterococcus faecalis V583]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Download         Length: 363 bp

>T20927 NC_004669:c58752-58390 [Enterococcus faecalis V583]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10059.46 Da        Isoelectric Point: 5.1585

>AT20927 WP_000245205.1 NC_004669:c59009-58746 [Enterococcus faecalis V583]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Download         Length: 264 bp

>AT20927 NC_004669:c59009-58746 [Enterococcus faecalis V583]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3H4V2

Antitoxin

Source ID Structure
AlphaFold DB R3H5D1

References