Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ImmA-IrrE/HTH_19(antitoxin)
Location 132769..133788 Replicon chromosome
Accession NC_004668
Organism Enterococcus faecalis V583

Toxin (Protein)


Gene name IrrE Uniprot ID Q839N5
Locus tag EF_RS00575 Protein ID WP_002359283.1
Coordinates 132769..133407 (-) Length 213 a.a.

Antitoxin (Protein)


Gene name ImmA Uniprot ID -
Locus tag EF_RS00580 Protein ID WP_002359284.1
Coordinates 133414..133788 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF_RS00555 (EF0124) 128949..129278 - 330 WP_002399673.1 hypothetical protein -
EF_RS00560 (EF0125) 129645..130817 + 1173 WP_000195429.1 IS256-like element IS256 family transposase -
EF_RS00565 (EF0126) 130937..131554 + 618 WP_010714161.1 hypothetical protein -
EF_RS00570 (EF0127) 132152..132727 + 576 WP_002359282.1 restriction endonuclease -
EF_RS00575 (EF0128) 132769..133407 - 639 WP_002359283.1 ImmA/IrrE family metallo-endopeptidase Toxin
EF_RS00580 (EF0129) 133414..133788 - 375 WP_002359284.1 helix-turn-helix domain-containing protein Antitoxin
EF_RS00585 (EF0130) 134630..134812 - 183 WP_002359286.1 hypothetical protein -
EF_RS00590 (EF0131) 134979..135230 + 252 WP_002359287.1 hypothetical protein -
EF_RS00595 (EF0132) 135317..135643 + 327 WP_002359288.1 hypothetical protein -
EF_RS00600 (EF0133) 135643..136050 + 408 WP_002359289.1 DUF961 family protein -
EF_RS00605 (EF0134) 136200..136424 + 225 WP_002359290.1 hypothetical protein -
EF_RS00610 (EF0135) 136453..136644 + 192 WP_010773668.1 hypothetical protein -
EF_RS00615 (EF0136) 136626..137030 + 405 WP_002359292.1 hypothetical protein -
EF_RS00620 (EF0137) 137142..137474 + 333 WP_002359293.1 nucleotidyltransferase domain-containing protein -
EF_RS00625 (EF0138) 137464..137847 + 384 WP_002359294.1 DUF86 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - EF0149 124300..184722 60422
flank IS/Tn - - 129645..130817 1172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

IrrE N-terminal-like domain

(86-176)

Antitoxin

Cro/C1-type helix-turn-helix domain

(7-61)


Sequences

Toxin        


Download         Length: 213 a.a.        Molecular weight: 25186.21 Da        Isoelectric Point: 9.1676

>T20897 WP_002359283.1 NC_004668:c133407-132769 [Enterococcus faecalis V583]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDMPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILLK

Download         Length: 639 bp

>T20897 NC_004668:c133407-132769 [Enterococcus faecalis V583]
ATGATACTAAATAAGAAAAAATTATATTTCGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAACATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
GCCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTTCCTGAAGATTATCCTAAAGAATTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAAAGTATGCATTTAGTACTTATGATGATCTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACGATATAGTTCTCAAGATAAGAATTCTTGACGAATTAATCTATTCCCACAACTTATC
TTATAAACAAGCCAATCTTATTTATAATAGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTACTCAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14518.29 Da        Isoelectric Point: 4.8896

>AT20897 WP_002359284.1 NC_004668:c133788-133414 [Enterococcus faecalis V583]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE

Download         Length: 375 bp

>AT20897 NC_004668:c133788-133414 [Enterococcus faecalis V583]
ATGAAAACTTACGAAAAGATAAAACAATTAAGAGAAAATAGAGAAATCACTCAAAAAGAAATTTCAAACGCACTAGACAT
TAATGTTAGTGTATATAATAAAATAGAACTTGGAATACGTCCATTAAGAGAAGAAGAACTTACTGCTATTGCTGATTTTT
TTAATGTTTCAATTGATTATTTAACAGGACGCACTGATAACCCTACAACACCTTCAAATAATCAAAAAGAAGCTGGACAA
AATATTATTTCACATTTCAGATTAAACACTAATGATTTAAGTTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A059N6D0

Antitoxin

Source ID Structure

References