Detailed information of TA system TA106020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2714625..2715658
• Replicon: chromosome
• Accession: NZ_CP029612
• Organism: Enterococcus faecalis strain H25

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: E2YSW3
• Locus tag: CNQ40_RS14475
• Protein ID: WP_002380879.1
• Coordinates: 2715005..2715658 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CNQ40_RS14470
• Protein ID: WP_002380877.1
• Coordinates: 2714625..2715008 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ40_RS14420	2709640..2710020	-	381	WP_002371429.1	DUF86 domain-containing protein	-
CNQ40_RS14425	2710010..2710336	-	327	WP_002393071.1	nucleotidyltransferase domain-containing protein	-
CNQ40_RS14430	2710396..2710689	-	294	WP_002394269.1	hypothetical protein	-
CNQ40_RS16655	2710731..2710883	-	153	WP_002380874.1	hypothetical protein	-
CNQ40_RS14440	2711110..2711298	+	189	WP_002371434.1	hypothetical protein	-
CNQ40_RS16660	2711285..2711446	-	162	WP_002380875.1	hypothetical protein	-
CNQ40_RS14445	2711469..2711885	-	417	WP_002380876.1	DUF961 family protein	-
CNQ40_RS14450	2711885..2712211	-	327	WP_002365226.1	hypothetical protein	-
CNQ40_RS14455	2712297..2712548	-	252	WP_002365227.1	hypothetical protein	-
CNQ40_RS14460	2712719..2713891	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
CNQ40_RS14470	2714625..2715008	+	384	WP_002380877.1	helix-turn-helix transcriptional regulator	Antitoxin
CNQ40_RS14475	2715005..2715658	+	654	WP_002380879.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CNQ40_RS14480	2715697..2716032	+	336	WP_002365231.1	helix-turn-helix transcriptional regulator	-
CNQ40_RS14485	2716070..2717401	-	1332	WP_002380880.1	FAD-dependent oxidoreductase	-
CNQ40_RS14490	2717501..2718436	-	936	WP_002393072.1	aldo/keto reductase	-
CNQ40_RS14495	2718452..2718871	-	420	WP_002380882.1	MerR family transcriptional regulator	-
CNQ40_RS14500	2718888..2719469	-	582	WP_002380883.1	phosphoglycerate mutase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2645983..2715915	69932
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_004668 Enterococcus faecalis V583 IS/Tn TA020897 129645..130817 0	flank	IS/Tn	-	-	2712719..2713891	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26064.07 Da
• Isoelectric Point: 6.7559

>T106020 WP_002380879.1 NZ_CP029612:2715005-2715658 [Enterococcus faecalis]
MIGLLLVDFISKKLYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T106020 NZ_CP029612:2715005-2715658 [Enterococcus faecalis]
TTGATTGGACTGTTGTTGGTGGATTTTATTAGTAAAAAACTCTATTTTGAAGCATTTAATTTTTCAAACGAATTAATAAA
AGAAGTATCTTACTATTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
ATGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATCTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAAGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAGGTTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACTCATACTTTTTCTGATATTCTTTTGAAAAATGGTTACTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGTATGTTAATGGCAAATGATCAAGCATTGTTTTACGCATTAAAAAAATTT
TATTCATTCGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATCGAGCATCTTATGTA
TGTAAATAATTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTCAATTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15031.09 Da
• Isoelectric Point: 5.8480

>AT106020 WP_002380877.1 NZ_CP029612:2714625-2715008 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMNIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD

Nucleotide

• Length: 384 bp

>AT106020 NZ_CP029612:2714625-2715008 [Enterococcus faecalis]
GTGCAACCATTAGCAAAAAGACTACAGCTTTTAAGAGAAGAAAAAGAATGGACAAAAACATATGTTGCAAAACAACTAGG
TTTAAATAACTTAGGAACCTATGCAAACTGGGAATATGGAACACGTGAACCGGATTCGGAAATGCTATCAAAAATAGCTT
CCCTATATGATGTATCTACCGATTTTTTACTAGGTAGAACAGAGGAAAGAATACATCTAAACGACAAGGAAAAAATTGGA
AAAAATATCATTTCTCATTTTCGTTTAAATACTTCTGATATGAACATAGAAGATATAGAAGAATTAGAAGAAGAATTAAT
CGATTTTCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGAAACGTAACAAAAAAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0M2A818