Detailed information of TA system TA123748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 132770..133789
• Replicon: chromosome
• Accession: NZ_CP039296
• Organism: Enterococcus faecalis strain VE14089

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: Q839N5
• Locus tag: E7T02_RS00615
• Protein ID: WP_002359283.1
• Coordinates: 132770..133408 (-)
• Length: 213 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: E7T02_RS00620
• Protein ID: WP_002359284.1
• Coordinates: 133415..133789 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7T02_RS00590	128949..129278	-	330	WP_002399673.1	hypothetical protein	-
E7T02_RS00600	129645..130817	+	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
E7T02_RS00605	130937..131554	+	618	WP_010714161.1	hypothetical protein	-
E7T02_RS00610	132153..132728	+	576	WP_002359282.1	restriction endonuclease	-
E7T02_RS00615	132770..133408	-	639	WP_002359283.1	ImmA/IrrE family metallo-endopeptidase	Toxin
E7T02_RS00620	133415..133789	-	375	WP_002359284.1	helix-turn-helix domain-containing protein	Antitoxin
E7T02_RS00625	134631..134813	-	183	WP_002359286.1	hypothetical protein	-
E7T02_RS00630	134980..135231	+	252	WP_002359287.1	hypothetical protein	-
E7T02_RS00635	135318..135644	+	327	WP_002359288.1	hypothetical protein	-
E7T02_RS00640	135644..136051	+	408	WP_002359289.1	DUF961 family protein	-
E7T02_RS00645	136201..136425	+	225	WP_002359290.1	hypothetical protein	-
E7T02_RS00650	136454..136645	+	192	WP_010773668.1	hypothetical protein	-
E7T02_RS00655	136627..137031	+	405	WP_002359292.1	hypothetical protein	-
E7T02_RS00660	137143..137475	+	333	WP_002359293.1	nucleotidyltransferase domain-containing protein	-
E7T02_RS00665	137465..137848	+	384	WP_002359294.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_004668 Enterococcus faecalis V583 Integrative and Conjugative Element TA020897 124300..184722 0	inside	Integrative and Conjugative Element	-	EF0149	124300..184722	60422
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_004668 Enterococcus faecalis V583 IS/Tn TA020897 129645..130817 0	flank	IS/Tn	-	-	129645..130817	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (86-176)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 213 a.a.
• Molecular weight: 25186.21 Da
• Isoelectric Point: 9.1676

>T123748 WP_002359283.1 NZ_CP039296:c133408-132770 [Enterococcus faecalis]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDMPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILLK

Nucleotide

• Length: 639 bp

>T123748 NZ_CP039296:c133408-132770 [Enterococcus faecalis]
ATGATACTAAATAAGAAAAAATTATATTTCGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAACATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
GCCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTTCCTGAAGATTATCCTAAAGAATTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAAAGTATGCATTTAGTACTTATGATGATCTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACGATATAGTTCTCAAGATAAGAATTCTTGACGAATTAATCTATTCCCACAACTTATC
TTATAAACAAGCCAATCTTATTTATAATAGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTACTCAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14518.29 Da
• Isoelectric Point: 4.8896

>AT123748 WP_002359284.1 NZ_CP039296:c133789-133415 [Enterococcus faecalis]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE

Nucleotide

• Length: 375 bp

>AT123748 NZ_CP039296:c133789-133415 [Enterococcus faecalis]
ATGAAAACTTACGAAAAGATAAAACAATTAAGAGAAAATAGAGAAATCACTCAAAAAGAAATTTCAAACGCACTAGACAT
TAATGTTAGTGTATATAATAAAATAGAACTTGGAATACGTCCATTAAGAGAAGAAGAACTTACTGCTATTGCTGATTTTT
TTAATGTTTCAATTGATTATTTAACAGGACGCACTGATAACCCTACAACACCTTCAAATAATCAAAAAGAAGCTGGACAA
AATATTATTTCACATTTCAGATTAAACACTAATGATTTAAGTTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A059N6D0