Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 2656265..2656688 Replicon chromosome
Accession NZ_CP077853
Organism Staphylococcus aureus strain N5

Toxin (Protein)


Gene name higB Uniprot ID A0A5S9I4Y6
Locus tag KU499_RS13120 Protein ID WP_001065091.1
Coordinates 2656265..2656516 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0E0VQM8
Locus tag KU499_RS13125 Protein ID WP_000028422.1
Coordinates 2656506..2656688 (+) Length 61 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU499_RS13075 (2651920) 2651920..2652840 + 921 WP_000138472.1 recombinase RecT -
KU499_RS13080 (2652921) 2652921..2653538 + 618 WP_071621397.1 MBL fold metallo-hydrolase -
KU499_RS13085 (2653539) 2653539..2654009 + 471 WP_000934764.1 single-stranded DNA-binding protein -
KU499_RS13090 (2654039) 2654039..2654923 + 885 WP_000148316.1 DnaD domain protein -
KU499_RS13095 (2654930) 2654930..2655148 + 219 WP_000338531.1 hypothetical protein -
KU499_RS13100 (2655157) 2655157..2655466 + 310 Protein_2539 RusA family crossover junction endodeoxyribonuclease -
KU499_RS13105 (2655479) 2655479..2655847 + 369 WP_000101274.1 SA1788 family PVL leukocidin-associated protein -
KU499_RS13110 (2655851) 2655851..2656093 + 243 WP_000131366.1 SAV1978 family virulence-associated passenger protein -
KU499_RS13115 (2656105) 2656105..2656272 + 168 WP_001624242.1 hypothetical protein -
KU499_RS13120 (2656265) 2656265..2656516 + 252 WP_001065091.1 DUF1024 family protein Toxin
KU499_RS13125 (2656506) 2656506..2656688 + 183 WP_000028422.1 hypothetical protein Antitoxin
KU499_RS13130 (2656681) 2656681..2657223 + 543 WP_000185659.1 dUTP pyrophosphatase -
KU499_RS13135 (2657260) 2657260..2657466 + 207 WP_000195803.1 DUF1381 domain-containing protein -
KU499_RS13140 (2657463) 2657463..2657849 + 387 WP_000592207.1 hypothetical protein -
KU499_RS13145 (2657846) 2657846..2657995 + 150 WP_000595265.1 transcriptional activator RinB -
KU499_RS13150 (2657995) 2657995..2658195 + 201 WP_000265043.1 DUF1514 family protein -
KU499_RS13155 (2658223) 2658223..2658639 + 417 WP_000590122.1 hypothetical protein -
KU499_RS13160 (2658871) 2658871..2659170 + 300 WP_000988336.1 HNH endonuclease -
KU499_RS13165 (2659300) 2659300..2659644 + 345 WP_000402904.1 hypothetical protein -
KU499_RS13170 (2659641) 2659641..2661302 + 1662 WP_000625088.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - hlb / sea / sak / chp / scn 2636791..2684356 47565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF1024

(1-82)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9349.22 Da        Isoelectric Point: 3.9473

>T208110 WP_001065091.1 NZ_CP077853:2656265-2656516 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Download         Length: 252 bp

>T208110 NZ_CP103570:2343228-2344550 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 61 a.a.        Molecular weight: 6741.63 Da        Isoelectric Point: 4.2658

>AT208110 WP_000028422.1 NZ_CP077853:2656506-2656688 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Download         Length: 183 bp

>AT208110 NZ_CP103570:2342962-2343228 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5S9I4Y6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VQM8

References