Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | abiEi-abiEii/AbiEii(toxin) |
| Location | 37824..39580 | Replicon | plasmid pDRC3B |
| Accession | NZ_CP064837 | ||
| Organism | Lactococcus lactis subsp. lactis strain DRC3 | ||
Toxin (Protein)
| Gene name | abiEi | Uniprot ID | Q48616 |
| Locus tag | LLDRC3_RS12885 | Protein ID | WP_012477748.1 |
| Coordinates | 38684..39580 (+) | Length | 299 a.a. |
Antitoxin (Protein)
| Gene name | abiEii | Uniprot ID | Q48615 |
| Locus tag | LLDRC3_RS12880 | Protein ID | WP_012477749.1 |
| Coordinates | 37824..38687 (+) | Length | 288 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LLDRC3_RS12860 (LLDRC3_pB37) | 34777..35019 | + | 243 | WP_012477753.1 | transposase | - |
| LLDRC3_RS12865 (LLDRC3_pB38) | 35432..35869 | + | 438 | WP_012477752.1 | IS3 family transposase | - |
| LLDRC3_RS12870 (LLDRC3_pB39) | 36116..36619 | - | 504 | WP_012477751.1 | type 1 glutamine amidotransferase domain-containing protein | - |
| LLDRC3_RS12875 (LLDRC3_pB40) | 36890..37471 | + | 582 | WP_012477750.1 | recombinase family protein | - |
| LLDRC3_RS12880 (LLDRC3_pB41) | 37824..38687 | + | 864 | WP_012477749.1 | AbiEi | Antitoxin |
| LLDRC3_RS12885 (LLDRC3_pB42) | 38684..39580 | + | 897 | WP_012477748.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| LLDRC3_RS12890 (LLDRC3_pB43) | 39744..40769 | - | 1026 | WP_012477747.1 | recombinase RecA | - |
| LLDRC3_RS12895 (LLDRC3_pB44) | 40782..41177 | - | 396 | WP_181409264.1 | hypothetical protein | - |
| LLDRC3_RS12900 (LLDRC3_pB45) | 41174..42640 | - | 1467 | WP_012477746.1 | Y-family DNA polymerase | - |
| LLDRC3_RS12905 (LLDRC3_pB46) | 42922..43950 | + | 1029 | WP_012477745.1 | Abi family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..64981 | 64981 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 299 a.a. Molecular weight: 35372.47 Da Isoelectric Point: 5.2182
>T181810 WP_012477748.1 NZ_CP064837:38684-39580 [Lactococcus lactis subsp. lactis]
MKNTRLKDLIATRNDDIGIENYRIRYATERFLTRLSASQYKEKFVLKGGFLIGVTYNLSQRTTKDLDTALIDFKSDAQSI
ERVITEICNIDLEDQVLFKLKELTSSQDMRIYPGYRAKLKMMFPDGNTRIDFDLDIGVGDRITPEAKKIKIPLIFNEVKG
VEKQIEVLAYPKETIQAEKLETILTRGKVNTRMKDYYDFHLLLTDQENSNSISFYYAFKNTWEFRNPTQFIDEELFEDWL
FILDEILESKELKEKYWPNYIKDRNYAKHLNMDDIISEIKEFVSKLKEEYIKENMSRN
MKNTRLKDLIATRNDDIGIENYRIRYATERFLTRLSASQYKEKFVLKGGFLIGVTYNLSQRTTKDLDTALIDFKSDAQSI
ERVITEICNIDLEDQVLFKLKELTSSQDMRIYPGYRAKLKMMFPDGNTRIDFDLDIGVGDRITPEAKKIKIPLIFNEVKG
VEKQIEVLAYPKETIQAEKLETILTRGKVNTRMKDYYDFHLLLTDQENSNSISFYYAFKNTWEFRNPTQFIDEELFEDWL
FILDEILESKELKEKYWPNYIKDRNYAKHLNMDDIISEIKEFVSKLKEEYIKENMSRN
Download Length: 897 bp
>T181810 NZ_CP086178:c2966079-2965711 [Streptomyces fungicidicus]
ATGTACTACCTCACCTTGCCCGAGTTGCTGAACCTCGCGAAGCGGCTCGGGGAGGACGTGGTGCGCGACTACGGGCTGCT
CGACTCGGCCTTGGCGCGTCCGCAGTCGAGCGTGTTCGGCCAGGACGCCTACCCGGACGTGTGGCAGAAGGCCGCGGCAC
TGATGGAGTCCCTCGCTCGCAACCATGCCCTCGTCGACGGGAACAAGCGCCTCGCCTGGTACGCGACCTGGGTCTTCCTG
CATATGAACGGGCATCCGCTCGACCCGGACTTCGACGTGGACGAGGCGGAGCAGTTCGTGCTGGACGTCTGCCAGGGCGC
ACTGGACGTCCCCAAGATCGCAGCTCAGTTGCCCCGCTTCGCGCGCTGA
ATGTACTACCTCACCTTGCCCGAGTTGCTGAACCTCGCGAAGCGGCTCGGGGAGGACGTGGTGCGCGACTACGGGCTGCT
CGACTCGGCCTTGGCGCGTCCGCAGTCGAGCGTGTTCGGCCAGGACGCCTACCCGGACGTGTGGCAGAAGGCCGCGGCAC
TGATGGAGTCCCTCGCTCGCAACCATGCCCTCGTCGACGGGAACAAGCGCCTCGCCTGGTACGCGACCTGGGTCTTCCTG
CATATGAACGGGCATCCGCTCGACCCGGACTTCGACGTGGACGAGGCGGAGCAGTTCGTGCTGGACGTCTGCCAGGGCGC
ACTGGACGTCCCCAAGATCGCAGCTCAGTTGCCCCGCTTCGCGCGCTGA
Antitoxin
Download Length: 288 a.a. Molecular weight: 33815.92 Da Isoelectric Point: 8.1894
>AT181810 WP_012477749.1 NZ_CP064837:37824-38687 [Lactococcus lactis subsp. lactis]
MDTYRISNLNLEQEDINNLKRISTNMLDTFNHEQLLSIIDVMKNTYFMNELSTYLVDDNLPDVGTEEFNFLVLANKYKGN
IIRKIVRDEGISDYYLRKFVLKYNLTEVDKGVYIFPHKKKDSLFIFQQKYSKAVISHETSLYLQDVIDYIPQKIQMSVPE
KYNISRIQEPHENRLTSYNYVDINSNNIMDKNIPINLVRNKSISPTQIETVNSFLGLPLRVTSIARSIVDVLKPSHKAEE
EVKEQAIKYYLERFPDNIVRLKRIAKTQNVLKELEYYLILLGVHYKL
MDTYRISNLNLEQEDINNLKRISTNMLDTFNHEQLLSIIDVMKNTYFMNELSTYLVDDNLPDVGTEEFNFLVLANKYKGN
IIRKIVRDEGISDYYLRKFVLKYNLTEVDKGVYIFPHKKKDSLFIFQQKYSKAVISHETSLYLQDVIDYIPQKIQMSVPE
KYNISRIQEPHENRLTSYNYVDINSNNIMDKNIPINLVRNKSISPTQIETVNSFLGLPLRVTSIARSIVDVLKPSHKAEE
EVKEQAIKYYLERFPDNIVRLKRIAKTQNVLKELEYYLILLGVHYKL
Download Length: 864 bp
>AT181810 NZ_CP086178:c2966276-2966079 [Streptomyces fungicidicus]
ATGGCTGGACTGAACCTGCGGTTTACTGACGAAGAGCTCGATGCCCTGCGTGAGCGTGCGGCTGCGGAAGGGCGCAGCAT
GCAGACCTTCGCGCACGACGCGGTGATCAAGGCCATAAACGAACACTCGAGGCTGTTCAACGAGGCGGCCGACCACGTGC
TGAGGGCCAGCGAAGAGCTGAACCGGAGGCTCGCCTGA
ATGGCTGGACTGAACCTGCGGTTTACTGACGAAGAGCTCGATGCCCTGCGTGAGCGTGCGGCTGCGGAAGGGCGCAGCAT
GCAGACCTTCGCGCACGACGCGGTGATCAAGGCCATAAACGAACACTCGAGGCTGTTCAACGAGGCGGCCGACCACGTGC
TGAGGGCCAGCGAAGAGCTGAACCGGAGGCTCGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|