6320

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type IV Classification (family/domain) abiEi-abiEii/-
Location 23014..24770 Replicon plasmid pNP40
Accession NC_010901
Organism Lactococcus lactis

Toxin (Protein)


Gene name abiEi Uniprot ID Q48616
Locus tag pNP40_p19 Protein ID WP_012477748.1
Coordinates 23014..23910 (-) Length 299 a.a.

Antitoxin (Protein)


Gene name abiEii Uniprot ID Q48615
Locus tag pNP40_p20 Protein ID WP_012477749.1
Coordinates 23907..24770 (-) Length 288 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS479_RS00085 (pNP40_p16) 18644..19672 - 1029 WP_012477745.1 Abi family protein -
HS479_RS00090 (pNP40_p17) 19954..21420 + 1467 WP_012477746.1 Y-family DNA polymerase -
HS479_RS00095 21483..21812 + 330 WP_238834587.1 hypothetical protein -
HS479_RS00100 (pNP40_p18) 21825..22850 + 1026 WP_012477747.1 recombinase RecA -
HS479_RS00105 (pNP40_p19) 23014..23910 - 897 WP_012477748.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
HS479_RS00110 (pNP40_p20) 23907..24770 - 864 WP_012477749.1 AbiEi Antitoxin
HS479_RS00115 (pNP40_p21) 25123..25704 - 582 WP_012477750.1 recombinase family protein -
HS479_RS00120 (pNP40_p22) 25975..26478 + 504 WP_012477751.1 type 1 glutamine amidotransferase domain-containing protein -
HS479_RS00125 (pNP40_p23) 26725..27162 - 438 WP_012477752.1 IS3 family transposase -
HS479_RS00130 (pNP40_p24) 27575..27817 - 243 WP_012477753.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..64980 64980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(30-250)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 299 a.a.        Molecular weight: 35372.47 Da        Isoelectric Point: 5.2182

>T6320 WP_012477748.1 NC_010901:c23910-23014 [Lactococcus lactis]
MKNTRLKDLIATRNDDIGIENYRIRYATERFLTRLSASQYKEKFVLKGGFLIGVTYNLSQRTTKDLDTALIDFKSDAQSI
ERVITEICNIDLEDQVLFKLKELTSSQDMRIYPGYRAKLKMMFPDGNTRIDFDLDIGVGDRITPEAKKIKIPLIFNEVKG
VEKQIEVLAYPKETIQAEKLETILTRGKVNTRMKDYYDFHLLLTDQENSNSISFYYAFKNTWEFRNPTQFIDEELFEDWL
FILDEILESKELKEKYWPNYIKDRNYAKHLNMDDIISEIKEFVSKLKEEYIKENMSRN

Download         Length: 897 bp

>T6320 NC_010901:c23910-23014 [Lactococcus lactis]
ATGAAAAATACAAGGTTAAAGGATTTAATTGCAACAAGAAATGATGATATTGGAATTGAAAATTATAGAATAAGATATGC
AACTGAAAGATTCTTAACAAGGCTTTCGGCAAGTCAATATAAGGAAAAATTTGTTTTAAAAGGAGGTTTTTTAATTGGTG
TCACCTATAACTTGAGTCAGAGGACTACTAAGGATTTAGATACAGCTCTGATAGACTTTAAAAGTGATGCACAGTCTATT
GAACGGGTAATTACTGAAATATGTAATATAGATTTAGAAGACCAAGTGCTTTTTAAATTAAAGGAACTTACAAGCAGTCA
AGATATGAGAATATATCCTGGGTACAGAGCTAAACTTAAGATGATGTTTCCTGATGGAAATACAAGGATAGACTTTGATC
TTGATATTGGAGTAGGAGATAGGATAACCCCAGAGGCTAAGAAAATAAAAATACCTCTAATATTTAATGAAGTAAAAGGT
GTAGAGAAACAGATAGAAGTATTAGCTTATCCAAAAGAAACTATTCAGGCAGAAAAATTAGAGACAATTCTCACTCGGGG
GAAAGTAAATACTAGAATGAAAGATTATTACGATTTTCATTTGCTTTTGACTGATCAAGAAAATAGTAATAGTATAAGTT
TTTATTATGCTTTTAAAAATACTTGGGAATTTAGAAACCCAACTCAATTTATTGATGAAGAACTGTTTGAAGATTGGCTC
TTTATTTTAGATGAGATACTTGAATCAAAAGAATTGAAAGAAAAGTATTGGCCAAACTATATTAAAGATAGAAATTATGC
TAAACATCTAAACATGGATGATATCATTTCAGAAATTAAAGAATTTGTTAGTAAATTAAAAGAAGAATATATAAAGGAGA
ATATGTCTAGAAATTAA

Antitoxin


Download         Length: 288 a.a.        Molecular weight: 33815.92 Da        Isoelectric Point: 8.1894

>AT6320 WP_012477749.1 NC_010901:c24770-23907 [Lactococcus lactis]
MDTYRISNLNLEQEDINNLKRISTNMLDTFNHEQLLSIIDVMKNTYFMNELSTYLVDDNLPDVGTEEFNFLVLANKYKGN
IIRKIVRDEGISDYYLRKFVLKYNLTEVDKGVYIFPHKKKDSLFIFQQKYSKAVISHETSLYLQDVIDYIPQKIQMSVPE
KYNISRIQEPHENRLTSYNYVDINSNNIMDKNIPINLVRNKSISPTQIETVNSFLGLPLRVTSIARSIVDVLKPSHKAEE
EVKEQAIKYYLERFPDNIVRLKRIAKTQNVLKELEYYLILLGVHYKL

Download         Length: 864 bp

>AT6320 NC_010901:c24770-23907 [Lactococcus lactis]
ATGGACACTTATAGAATATCAAACTTAAATCTAGAACAGGAAGATATAAATAATCTAAAGAGAATCTCAACCAATATGTT
AGATACTTTTAACCATGAACAGCTTCTTTCAATCATTGATGTGATGAAAAATACTTATTTCATGAATGAACTCTCAACCT
ATTTAGTAGACGATAATCTCCCTGATGTAGGTACTGAAGAATTTAATTTTTTGGTTCTTGCTAATAAATATAAAGGAAAT
ATAATAAGAAAAATAGTACGTGATGAAGGTATAAGTGATTACTATTTGAGAAAATTTGTTTTGAAGTATAATCTTACTGA
GGTTGACAAAGGTGTTTATATTTTTCCTCATAAAAAAAAGGACAGTTTATTTATTTTTCAGCAGAAATACAGCAAAGCAG
TTATCTCACATGAAACATCATTATATCTACAAGATGTAATAGATTATATTCCGCAAAAAATACAAATGAGCGTTCCAGAA
AAGTATAATATCAGTAGAATTCAGGAACCTCACGAAAATCGTTTAACAAGCTATAACTATGTGGATATTAACTCTAATAA
TATAATGGATAAAAATATTCCAATCAATTTAGTCAGAAATAAGAGTATTAGTCCTACACAAATAGAAACAGTAAATAGCT
TTTTAGGTCTCCCACTAAGAGTCACTTCTATTGCTCGGTCGATAGTAGACGTTTTAAAACCTTCCCACAAGGCTGAAGAA
GAAGTGAAAGAACAGGCGATTAAGTATTATTTAGAAAGATTTCCAGATAATATTGTGCGCTTAAAACGTATAGCTAAAAC
ACAAAATGTTTTAAAAGAACTAGAGTATTACTTGATTTTATTGGGAGTACATTATAAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q48616

Antitoxin

Source ID Structure
AlphaFold DB Q48615

References

(1) Ron L Dy et al. (2014) A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism. Nucleic Acids Research 42(7):4590-605. [PubMed:24465005] experimental


experimental experimental literature