Detailed information of TA system TA022930

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/-
• Location: 23014..24770
• Replicon: plasmid pNP40
• Accession: NC_010901
• Organism: Lactococcus lactis

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: Q48616
• Locus tag: HS479_RS00105
• Protein ID: WP_012477748.1
• Coordinates: 23014..23910 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: Q48615
• Locus tag: HS479_RS00110
• Protein ID: WP_012477749.1
• Coordinates: 23907..24770 (-)
• Length: 288 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS479_RS00085 (pNP40_p16)	18644..19672	-	1029	WP_012477745.1	Abi family protein	-
HS479_RS00090 (pNP40_p17)	19954..21420	+	1467	WP_012477746.1	Y-family DNA polymerase	-
HS479_RS00095	21483..21812	+	330	WP_238834587.1	hypothetical protein	-
HS479_RS00100 (pNP40_p18)	21825..22850	+	1026	WP_012477747.1	recombinase RecA	-
HS479_RS00105 (pNP40_p19)	23014..23910	-	897	WP_012477748.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HS479_RS00110 (pNP40_p20)	23907..24770	-	864	WP_012477749.1	AbiEi	Antitoxin
HS479_RS00115 (pNP40_p21)	25123..25704	-	582	WP_012477750.1	recombinase family protein	-
HS479_RS00120 (pNP40_p22)	25975..26478	+	504	WP_012477751.1	type 1 glutamine amidotransferase domain-containing protein	-
HS479_RS00125 (pNP40_p23)	26725..27162	-	438	WP_012477752.1	IS3 family transposase	-
HS479_RS00130 (pNP40_p24)	27575..27817	-	243	WP_012477753.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP064837 Lactococcus lactis subsp. lactis strain DRC3 Non-Mobilizable plasmid TA181810 1..64981 0	inside	Non-Mobilizable plasmid	-	-	1..64980	64980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (30-250)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 35372.47 Da
• Isoelectric Point: 5.2182

>T22930 WP_012477748.1 NC_010901:c23910-23014 [Lactococcus lactis]
MKNTRLKDLIATRNDDIGIENYRIRYATERFLTRLSASQYKEKFVLKGGFLIGVTYNLSQRTTKDLDTALIDFKSDAQSI
ERVITEICNIDLEDQVLFKLKELTSSQDMRIYPGYRAKLKMMFPDGNTRIDFDLDIGVGDRITPEAKKIKIPLIFNEVKG
VEKQIEVLAYPKETIQAEKLETILTRGKVNTRMKDYYDFHLLLTDQENSNSISFYYAFKNTWEFRNPTQFIDEELFEDWL
FILDEILESKELKEKYWPNYIKDRNYAKHLNMDDIISEIKEFVSKLKEEYIKENMSRN

Nucleotide

• Length: 897 bp

>T22930 NC_010901:c23910-23014 [Lactococcus lactis]
ATGAAAAATACAAGGTTAAAGGATTTAATTGCAACAAGAAATGATGATATTGGAATTGAAAATTATAGAATAAGATATGC
AACTGAAAGATTCTTAACAAGGCTTTCGGCAAGTCAATATAAGGAAAAATTTGTTTTAAAAGGAGGTTTTTTAATTGGTG
TCACCTATAACTTGAGTCAGAGGACTACTAAGGATTTAGATACAGCTCTGATAGACTTTAAAAGTGATGCACAGTCTATT
GAACGGGTAATTACTGAAATATGTAATATAGATTTAGAAGACCAAGTGCTTTTTAAATTAAAGGAACTTACAAGCAGTCA
AGATATGAGAATATATCCTGGGTACAGAGCTAAACTTAAGATGATGTTTCCTGATGGAAATACAAGGATAGACTTTGATC
TTGATATTGGAGTAGGAGATAGGATAACCCCAGAGGCTAAGAAAATAAAAATACCTCTAATATTTAATGAAGTAAAAGGT
GTAGAGAAACAGATAGAAGTATTAGCTTATCCAAAAGAAACTATTCAGGCAGAAAAATTAGAGACAATTCTCACTCGGGG
GAAAGTAAATACTAGAATGAAAGATTATTACGATTTTCATTTGCTTTTGACTGATCAAGAAAATAGTAATAGTATAAGTT
TTTATTATGCTTTTAAAAATACTTGGGAATTTAGAAACCCAACTCAATTTATTGATGAAGAACTGTTTGAAGATTGGCTC
TTTATTTTAGATGAGATACTTGAATCAAAAGAATTGAAAGAAAAGTATTGGCCAAACTATATTAAAGATAGAAATTATGC
TAAACATCTAAACATGGATGATATCATTTCAGAAATTAAAGAATTTGTTAGTAAATTAAAAGAAGAATATATAAAGGAGA
ATATGTCTAGAAATTAA

Antitoxin

Protein

• Length: 288 a.a.
• Molecular weight: 33815.92 Da
• Isoelectric Point: 8.1894

>AT22930 WP_012477749.1 NC_010901:c24770-23907 [Lactococcus lactis]
MDTYRISNLNLEQEDINNLKRISTNMLDTFNHEQLLSIIDVMKNTYFMNELSTYLVDDNLPDVGTEEFNFLVLANKYKGN
IIRKIVRDEGISDYYLRKFVLKYNLTEVDKGVYIFPHKKKDSLFIFQQKYSKAVISHETSLYLQDVIDYIPQKIQMSVPE
KYNISRIQEPHENRLTSYNYVDINSNNIMDKNIPINLVRNKSISPTQIETVNSFLGLPLRVTSIARSIVDVLKPSHKAEE
EVKEQAIKYYLERFPDNIVRLKRIAKTQNVLKELEYYLILLGVHYKL

Nucleotide

• Length: 864 bp

>AT22930 NC_010901:c24770-23907 [Lactococcus lactis]
ATGGACACTTATAGAATATCAAACTTAAATCTAGAACAGGAAGATATAAATAATCTAAAGAGAATCTCAACCAATATGTT
AGATACTTTTAACCATGAACAGCTTCTTTCAATCATTGATGTGATGAAAAATACTTATTTCATGAATGAACTCTCAACCT
ATTTAGTAGACGATAATCTCCCTGATGTAGGTACTGAAGAATTTAATTTTTTGGTTCTTGCTAATAAATATAAAGGAAAT
ATAATAAGAAAAATAGTACGTGATGAAGGTATAAGTGATTACTATTTGAGAAAATTTGTTTTGAAGTATAATCTTACTGA
GGTTGACAAAGGTGTTTATATTTTTCCTCATAAAAAAAAGGACAGTTTATTTATTTTTCAGCAGAAATACAGCAAAGCAG
TTATCTCACATGAAACATCATTATATCTACAAGATGTAATAGATTATATTCCGCAAAAAATACAAATGAGCGTTCCAGAA
AAGTATAATATCAGTAGAATTCAGGAACCTCACGAAAATCGTTTAACAAGCTATAACTATGTGGATATTAACTCTAATAA
TATAATGGATAAAAATATTCCAATCAATTTAGTCAGAAATAAGAGTATTAGTCCTACACAAATAGAAACAGTAAATAGCT
TTTTAGGTCTCCCACTAAGAGTCACTTCTATTGCTCGGTCGATAGTAGACGTTTTAAAACCTTCCCACAAGGCTGAAGAA
GAAGTGAAAGAACAGGCGATTAAGTATTATTTAGAAAGATTTCCAGATAATATTGTGCGCTTAAAACGTATAGCTAAAAC
ACAAAATGTTTTAAAAGAACTAGAGTATTACTTGATTTTATTGGGAGTACATTATAAACTATGA

Structures

Toxin

• AlphaFold DB: Q48616

Antitoxin

• AlphaFold DB: Q48615