127617

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3617691..3618385 Replicon chromosome
Accession NZ_CP041110
Organism Escherichia coli strain ECCTRSRTH03

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CI675_RS17635 Protein ID WP_001263489.1
Coordinates 3617987..3618385 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CI675_RS17630 Protein ID WP_000554758.1
Coordinates 3617691..3617984 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI675_RS17600 3613129..3613365 + 237 WP_000474084.1 DUF1471 domain-containing protein -
CI675_RS17605 3613510..3614490 + 981 WP_000019402.1 IS5-like element IS5 family transposase -
CI675_RS17610 3614637..3614711 + 75 Protein_3427 RNA polymerase sigma factor FliA -
CI675_RS17615 3614724..3615587 + 864 WP_001169518.1 flagellar motor stator protein MotA -
CI675_RS17620 3615590..3616513 + 924 WP_001232546.1 putative lateral flagellar export/assembly protein LafU -
CI675_RS17625 3616584..3617639 + 1056 WP_001226164.1 DNA polymerase IV -
CI675_RS17630 3617691..3617984 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CI675_RS17635 3617987..3618385 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CI675_RS17640 3618395..3618847 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
CI675_RS17645 3619165..3619371 + 207 Protein_3434 RtcB family protein -
CI675_RS17650 3619367..3619888 + 522 Protein_3435 peptide chain release factor H -
CI675_RS17655 3619945..3621402 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CI675_RS17660 3621663..3622121 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 3622717..3622797 + 81 NuclAT_11 - -
- 3622717..3622797 + 81 NuclAT_11 - -
- 3622717..3622797 + 81 NuclAT_11 - -
- 3622717..3622797 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3610615..3636809 26194
flank IS/Tn - - 3613510..3614490 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T127617 WP_001263489.1 NZ_CP041110:3617987-3618385 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T127617 NZ_CP041110:3617987-3618385 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT127617 WP_000554758.1 NZ_CP041110:3617691-3617984 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT127617 NZ_CP041110:3617691-3617984 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References