Detailed information of TA system TA010167

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): orf9-orf10/-
• Location: 9588..10972
• Replicon: plasmid pBsph
• Accession: NC_010381
• Organism: Lysinibacillus sphaericus C3-41

Toxin (Protein)

• Gene name: ORF9
• Uniprot ID: -
• Locus tag: BSPH_RS22485
• Protein ID: WP_012291646.1
• Coordinates: 9588..10133 (+)
• Length: 182 a.a.

Antitoxin (Protein)

• Gene name: ORF10
• Uniprot ID: B1I081
• Locus tag: BSPH_RS22490
• Protein ID: WP_012291647.1
• Coordinates: 10133..10972 (+)
• Length: 280 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSPH_RS22455 (Bsph_p003)	4837..5592	+	756	WP_012291640.1	hypothetical protein	-
BSPH_RS22460 (Bsph_p004)	5781..6875	+	1095	WP_041659270.1	hypothetical protein	-
BSPH_RS22465 (Bsph_p005)	6879..8015	+	1137	WP_012291642.1	hypothetical protein	-
BSPH_RS22470 (Bsph_p006)	7999..8301	+	303	WP_145912404.1	DUF1292 domain-containing protein	-
BSPH_RS22475 (Bsph_p007)	8288..8839	+	552	WP_145912405.1	ATP-binding protein	-
BSPH_RS22480 (Bsph_p008)	8879..9367	+	489	WP_012291645.1	hypothetical protein	-
BSPH_RS22485 (Bsph_p009)	9588..10133	+	546	WP_012291646.1	NYN domain-containing protein	Toxin
BSPH_RS22490 (Bsph_p010)	10133..10972	+	840	WP_012291647.1	hypothetical protein	Antitoxin
BSPH_RS22495 (Bsph_p011)	11099..11950	+	852	WP_012291648.1	hypothetical protein	-
BSPH_RS22500 (Bsph_p012)	11995..12270	+	276	WP_041659272.1	hypothetical protein	-
BSPH_RS22505 (Bsph_p013)	12558..12926	+	369	WP_031417274.1	hypothetical protein	-
BSPH_RS22510 (Bsph_p014)	13367..14410	+	1044	WP_012291651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP014644 Lysinibacillus sphaericus strain OT4b.25 Non-Mobilizable plasmid TA061619 1..176083 0.006 View NZ_CP014857 Lysinibacillus sphaericus strain III(3)7 Non-Mobilizable plasmid TA061845, TA061846 1..173793 0.006	inside	Non-Mobilizable plasmid	-	-	1..177642	177642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• NYN domain (4-158)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 182 a.a.
• Molecular weight: 20304.38 Da
• Isoelectric Point: 9.4376

>T10167 WP_012291646.1 NC_010381:9588-10133 [Lysinibacillus sphaericus C3-41]
MKKTAILVDEMNVIQQLRRLGIHGFNPWNAFYQALQRWNGDKKGVKAFYCANVPKEDAEHEKRNNFFEGLINHNIKVSVG
TVVYDSNSRRIAKGVDVALALDIVEHARSGVQDIFICTGDADVVPAIIRAQELGSRVHVVVSRAIPAKNITLAADSVVRL
EDILETMPPHRIKYFAKERVS

Nucleotide

• Length: 546 bp

>T10167 NC_010381:9588-10133 [Lysinibacillus sphaericus C3-41]
ATGAAAAAGACAGCTATTTTAGTAGATGAAATGAACGTGATTCAACAACTCCGACGTTTAGGAATCCATGGATTCAATCC
CTGGAATGCATTTTATCAGGCGTTACAACGTTGGAATGGTGATAAGAAAGGTGTTAAAGCATTTTATTGTGCTAACGTAC
CTAAAGAAGATGCAGAACATGAAAAGCGTAATAATTTCTTTGAAGGATTAATAAATCATAACATCAAAGTATCAGTAGGT
ACTGTTGTATATGACTCAAACTCAAGACGGATAGCAAAAGGTGTAGATGTTGCCTTAGCGCTGGACATAGTAGAACATGC
TAGAAGCGGTGTACAAGATATTTTCATCTGTACAGGTGATGCTGATGTTGTGCCTGCCATTATTCGCGCACAGGAGCTCG
GTAGTCGTGTTCACGTTGTAGTTTCCAGGGCTATCCCAGCTAAAAATATCACCCTTGCAGCTGATTCAGTGGTTAGATTA
GAGGATATCTTAGAAACCATGCCTCCTCACCGCATTAAATACTTTGCTAAGGAGCGTGTGAGCTAA

Antitoxin

Protein

• Length: 280 a.a.
• Molecular weight: 31528.09 Da
• Isoelectric Point: 5.2827

>AT10167 WP_012291647.1 NC_010381:10133-10972 [Lysinibacillus sphaericus C3-41]
METAPQEIFDLTLAKKTENEKELENAYRRIPDEYHDFIVLLEQKIKITDSRYFLAEEFITMGAYQDEKGIYLKIKKPYMT
VDGRIRMARDEHSKKEAKLIISEPQIIQVGNRICMNVTVESEIHGTATGTIEVNINGFGVDKKNPVANAQTSALGRALGF
LGYGIIGTAATASLDEATNLYNSLFASPSKEQVDPKKPEIKRVRICGPIEYKNNDLGYVQIQLETEEYVIMQIGKSLFRY
ENILVTNAVVKVRGWWNGEIFAAAPDQDIILDQNKKIVS

Nucleotide

• Length: 840 bp

>AT10167 NC_010381:10133-10972 [Lysinibacillus sphaericus C3-41]
ATGGAGACAGCGCCACAAGAGATATTTGACCTTACACTTGCCAAAAAGACTGAAAATGAAAAGGAATTAGAGAATGCTTA
CAGAAGGATTCCGGATGAATACCATGACTTTATTGTATTGCTGGAGCAAAAAATTAAAATTACTGATAGTAGATACTTTT
TAGCAGAGGAATTTATAACAATGGGTGCCTACCAAGACGAAAAAGGTATCTATTTAAAGATTAAAAAGCCGTATATGACT
GTTGATGGCCGTATTCGAATGGCTCGCGATGAACATTCTAAAAAGGAAGCCAAACTAATTATTTCTGAACCTCAAATTAT
ACAAGTGGGAAATCGAATTTGTATGAATGTGACAGTTGAAAGTGAAATCCATGGCACTGCTACAGGAACTATAGAGGTTA
ATATTAACGGTTTTGGGGTAGATAAGAAGAATCCTGTAGCTAACGCACAAACTAGCGCTTTAGGACGTGCATTAGGCTTT
TTAGGGTACGGTATTATAGGTACTGCAGCAACCGCTTCATTAGATGAGGCTACAAACCTATATAACTCTTTATTTGCTTC
TCCAAGTAAGGAACAAGTTGATCCAAAGAAACCCGAGATTAAACGAGTTAGAATTTGTGGACCGATCGAATATAAAAACA
ACGACTTAGGGTATGTTCAAATACAGCTAGAAACTGAGGAATATGTAATAATGCAAATTGGAAAAAGCTTATTCAGATAT
GAAAATATACTGGTAACAAATGCAGTAGTAAAGGTGCGTGGTTGGTGGAACGGTGAAATATTCGCAGCTGCACCTGACCA
GGATATTATCTTGGACCAAAATAAAAAGATTGTTTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6H0A0V0

References

(1) Pan Fu et al. (2018) A toxin-antitoxin system is essential for the stability of mosquitocidal plasmid pBsph of Lysinibacillus sphaericus. Microbiological Research 214:114-122. [PubMed:30031473]


 experimental literature